Abstract
Mononuclear cell infiltration and alteration in the connective tissues are prominent features of the inflammatory response in a number of diseases. To determine whether mononuclear cell products can modulate collagen synthesis, human peripheral mononuclear cells from normal donors were isolated by Ficoll-Hypaque gradient centrifugation and then incubated for 48 h with or without phytohemagglutinin. Confluent cultures of normal, human skin fibroblasts were incubated with [14C]proline and various amounts of dialyzed supernates from the mononuclear cell cultures. Labeled, newly synthesized collagen was estimated by [14C]hydroxyproline analysis, collagenase digestion, and chromatography on Agarose A-5m in sodium dodecyl sulfate. The total incorporation of [14C]proline was not significantly affected by addition of the mononuclear cell supernates, but as much as 90% decrease in the synthesis by the fibroblasts of labeled collagen was found relative to controls. Supernates from the phytohemagglutinin- stimulated cultures were more active than those from nonstimulated cells. These results suggest that mononuclear cells can synthesize a factor(s) which can selectively inhibit collagen synthesis.
Full Text
The Full Text of this article is available as a PDF (669.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aalto M., Potila M., Kulonen E. The effect of silica-treated macrophages on the synthesis of collagen and other proteins in vitro. Exp Cell Res. 1976 Jan;97:193–202. doi: 10.1016/0014-4827(76)90668-6. [DOI] [PubMed] [Google Scholar]
- Bashey R. I., Jimenez S. A. Increased sensitivity of scleroderma fibroblasts in culture to stimulation of protein and collagen synthesis by serum. Biochem Biophys Res Commun. 1977 Jun 20;76(4):1214–1222. doi: 10.1016/0006-291x(77)90985-8. [DOI] [PubMed] [Google Scholar]
- Bańkowski E., Mitchell W. M. Human procollagen I. An anionic tropocollagen precursor from skin fibroblasts in culture. Biophys Chem. 1973 Dec;1(2):73–86. doi: 10.1016/0301-4622(73)80003-1. [DOI] [PubMed] [Google Scholar]
- Blanck T. J., Peterkofsky B. The stimulation of collagen secretion by ascorbate as a result of increased proline hydroxylation in chick embryo fibroblasts. Arch Biochem Biophys. 1975 Nov;171(1):259–267. doi: 10.1016/0003-9861(75)90031-4. [DOI] [PubMed] [Google Scholar]
- Burke J. M., Ross R. Collagen synthesis by monkey arterial smooth muscle cells during proliferation and quiescence in culture. Exp Cell Res. 1977 Jul;107(2):387–395. doi: 10.1016/0014-4827(77)90360-3. [DOI] [PubMed] [Google Scholar]
- Böyum A. Isolation of leucocytes from human blood. Further observations. Methylcellulose, dextran, and ficoll as erythrocyteaggregating agents. Scand J Clin Lab Invest Suppl. 1968;97:31–50. [PubMed] [Google Scholar]
- Carrel A. GROWTH-PROMOTING FUNCTION OF LEUCOCYTES. J Exp Med. 1922 Sep 30;36(4):385–391. doi: 10.1084/jem.36.4.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen L. B., Buchanan J. M. Mitogenic activity of blood components. I. Thrombin and prothrombin. Proc Natl Acad Sci U S A. 1975 Jan;72(1):131–135. doi: 10.1073/pnas.72.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feng J., Melcher A. H., Brunette D. M., Moe H. K. Determination of L-ascorbic acid levels in culture medium: concentrations in commercial media and maintenance of levels under conditions of organ culture. In Vitro. 1977 Feb;13(2):91–99. doi: 10.1007/BF02615072. [DOI] [PubMed] [Google Scholar]
- Gospodarowicz D., Moran J. S. Growth factors in mammalian cell culture. Annu Rev Biochem. 1976;45:531–558. doi: 10.1146/annurev.bi.45.070176.002531. [DOI] [PubMed] [Google Scholar]
- Heppleston A. G., Styles J. A. Activity of a macrophage factor in collagen formation by silica. Nature. 1967 Apr 29;214(5087):521–522. doi: 10.1038/214521a0. [DOI] [PubMed] [Google Scholar]
- Jimenez S. A., Bashey R. I. Collagen synthesis by scleroderma fibroblasts in culture. Arthritis Rheum. 1977 Apr;20(3):902–903. doi: 10.1002/art.1780200323. [DOI] [PubMed] [Google Scholar]
- Jimenez S. A., Dehm P., Prockop D. J. Further evidence for a transport form of collagen. Its extrusion and extracellular conversion to tropocollagen in embryonic tendon. FEBS Lett. 1971 Oct 1;17(2):245–248. doi: 10.1016/0014-5793(71)80156-4. [DOI] [PubMed] [Google Scholar]
- Johnson R. L., Ziff M. Lymphokine stimulation of collagen accumulation. J Clin Invest. 1976 Jul;58(1):240–252. doi: 10.1172/JCI108455. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Juva K., Prockop D. J. Modified procedure for the assay of H-3-or C-14-labeled hydroxyproline. Anal Biochem. 1966 Apr;15(1):77–83. doi: 10.1016/0003-2697(66)90249-1. [DOI] [PubMed] [Google Scholar]
- Miyoshi M., Rosenbloom J. General proteolytic activity of highly purified preparations of clostridial collagenase. Connect Tissue Res. 1974;2(2):77–84. doi: 10.3109/03008207409152091. [DOI] [PubMed] [Google Scholar]
- Narayanan A. S., Page R. C. Serum modulates collagen types in human gingiva fibroblasts. FEBS Lett. 1977 Aug 1;80(1):221–224. doi: 10.1016/0014-5793(77)80444-4. [DOI] [PubMed] [Google Scholar]
- Nolan J. C., Cardinale G. J., Undenfriend S. The formation of hydroxyproline in collagen by cells grown in the absence of serum. Biochim Biophys Acta. 1978 Sep 21;543(1):116–122. doi: 10.1016/0304-4165(78)90459-2. [DOI] [PubMed] [Google Scholar]
- Wahl S. M., Wahl L. M., McCarthy J. B. Lymphocyte-mediated activation of fibroblast proliferation and collagen production. J Immunol. 1978 Sep;121(3):942–946. [PubMed] [Google Scholar]
- Wiebel F., Baserga R. Early alterations in amino acid pools and protein synthesis of diploid fibroblasts stimulated to synthesize DNA by addition of serum. J Cell Physiol. 1969 Oct;74(2):191–202. doi: 10.1002/jcp.1040740211. [DOI] [PubMed] [Google Scholar]