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. 1979 Dec 1;150(6):1498–1509. doi: 10.1084/jem.150.6.1498

Characterization and localization of the enzymatic deacylation of lipoteichoic acid in group A streptococci

PMCID: PMC2185733  PMID: 390087

Abstract

Protoplasts of a group A streptococcal strain were shown to contain enzymatic activity capable of converting lipoteichoic acid (LTA) to deacylated lipoteichoic acid (dLTA). The enzyme(s) appear to be located mainly in the membrane, although activity was also found in the cytoplasm. Determination of the sites of cleavage within the LTA molecule was approached by comparing the chemical composition of LTA and native dLTA. Native dLTA, as distinguished from chemically deacylated LTA, was isolated from buffer in which live streptococci had been resuspended and incubated. The chemical data suggest that the enzyme(s) was(were) lipolytic in nature; that is, the conversion of LTA to dLTA was the result of cleavage of the ester linkages between the fatty acids and the remainder of the LTA molecule.

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Selected References

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  1. Alkan M. L., Beachey E. H. Excretion of lipoteichoic acid by group A streptococci. Influence of penicillin on excretion and loss of ability to adhere to human oral mucosal cells. J Clin Invest. 1978 Mar;61(3):671–677. doi: 10.1172/JCI108979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beachey E. H. Binding of group A streptococci to human oral mucosal cells by lipoteichoic acid. Trans Assoc Am Physicians. 1975;88:285–292. [PubMed] [Google Scholar]
  3. Beachey E. H., Chiang T. M., Ofek I., Kang A. H. Interaction of lipoteichoic acid of group A streptococci with human platelets. Infect Immun. 1977 May;16(2):649–654. doi: 10.1128/iai.16.2.649-654.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Emdur L. I., Chiu T. H. Turnover of phosphatidylglycerol in Streptococcus sanguis. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1137–1144. doi: 10.1016/s0006-291x(74)80097-5. [DOI] [PubMed] [Google Scholar]
  5. Glaser L., Lindsay B. The synthesis of lipoteichoic acid carrier. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1131–1136. doi: 10.1016/s0006-291x(74)80096-3. [DOI] [PubMed] [Google Scholar]
  6. Horne D., Tomasz A. Release of lipoteichoic acid from Streptococcus sanguis: stimulation of release during penicillin treatment. J Bacteriol. 1979 Mar;137(3):1180–1184. doi: 10.1128/jb.137.3.1180-1184.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Joseph R., Shockman G. D. Synthesis and excretion of glycerol teichoic acid during growth of two streptococcal species. Infect Immun. 1975 Aug;12(2):333–338. doi: 10.1128/iai.12.2.333-338.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kessler R. E., Shockman G. D. Enzymatic deacylation of lipoteichoic acid by protoplasts of Streptococcus faecium (Streptococcus faecalis ATCC 9790). J Bacteriol. 1979 Mar;137(3):1176–1179. doi: 10.1128/jb.137.3.1176-1179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kessler R. E., Shockman G. D. Precursor-product relationship of intracellular and extracellular lipoteichoic acids of Streptococcus faecium. J Bacteriol. 1979 Feb;137(2):869–877. doi: 10.1128/jb.137.2.869-877.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kessler R. E., van de Rijn I. Quantitative immunoelectrophoretic analysis of Streptococcus pyogenes membrane. Infect Immun. 1979 Dec;26(3):892–902. doi: 10.1128/iai.26.3.892-902.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  12. MCCARTY M. THE ROLE OF D-ALANINE IN THE SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL GLYCEROL TEICHOIC ACID. Proc Natl Acad Sci U S A. 1964 Aug;52:259–265. doi: 10.1073/pnas.52.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Markham J. L., Knox K. W., Wicken A. J., Hewett M. J. Formation of extracellular lipoteichoic acid by oral streptococci and lactobacilli. Infect Immun. 1975 Aug;12(2):378–386. doi: 10.1128/iai.12.2.378-386.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McLean C., Werner D. A., Aminoff D. Quantitative determination of reducing sugars, oligosaccharides, and glycoproteins with (3H)borohydride. Anal Biochem. 1973 Sep;55(1):72–84. doi: 10.1016/0003-2697(73)90291-1. [DOI] [PubMed] [Google Scholar]
  15. Owen P., Salton M. R. Antigenic and enzymatic architecture of Micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3711–3715. doi: 10.1073/pnas.72.9.3711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Slabyj B. M., Panos C. Membrane lipoteichoic acid of Streptococcus pyogenes and its stabilized L-form and the effect of two antibiotics upon its cellular content. J Bacteriol. 1976 Aug;127(2):855–862. doi: 10.1128/jb.127.2.855-862.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Driel D., Wicken A. J., Dickson M. R., Knox K. W. Cellular location of the lipoteichoic acids of Lactobacillus fermenti NCTC 6991 and Lactobacillus casei NCTC 6375. J Ultrastruct Res. 1973 Jun;43(5):483–497. doi: 10.1016/s0022-5320(73)90025-7. [DOI] [PubMed] [Google Scholar]
  18. WILSON A. T., WILEY G. G. THE CELLULAR ANTIGENS OF GROUP A STREPTOCOCCI; IMMUNOELECTROPHORETIC STUDIES OF THE C, M, T, PGP, E4, F, AND E ANTIGENS OF SEROTYPE 17 STREPTOCOCCI. J Exp Med. 1963 Oct 1;118:527–556. doi: 10.1084/jem.118.4.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weeke B. Crossed immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:47–56. doi: 10.1111/j.1365-3083.1973.tb03778.x. [DOI] [PubMed] [Google Scholar]
  20. Wicken A. J., Gibbens J. W., Knox K. W. Comparative studies on the isolation of membrane lipoteichoic acid from Lactobacillus fermenti. J Bacteriol. 1973 Jan;113(1):365–372. doi: 10.1128/jb.113.1.365-372.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van de Rijn I., Kessler R. E. Chemical analysis of changes in membrane composition during growth of Streptococcus pyogenes. Infect Immun. 1979 Dec;26(3):883–891. doi: 10.1128/iai.26.3.883-891.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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