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. 1980 Jan 1;151(1):224–234. doi: 10.1084/jem.151.1.224

Synergistic genetic defect in B-lymphocyte function. I. Defective responses to B-cell stimulants and their genetic basis

PMCID: PMC2185770  PMID: 6965307

Abstract

CBA/N female mice, which express an X-linked defect in B-lymphocyte function, were mated with C3H/HeJ male mice, which are unresponsive to lipopolysaccharide (LPS). The resulting F1 hybrid females were mated to C3H/HeJ males. Approximately one-half of the backcross (BC.1) males obtained from this mating expressed a more profound immunologic defect than either of the parental strains. Spleen cells from these mice were unresponsive to a series of B-cell mitogens including LPS prepared from Escherichia coli K235 and from E. coli 0111:B4, lipoprotein mitogen from E. coli, and Nocardia water-soluble mitogen (NWSM). They failed to give in vitro antibody responses to the thymus-independent type 2 (TI- 2) antigen trinophenylated Ficoll and most were unresponsive to the TI- 1 antigens trinitrophenylated Brucella abortus, trinitrophenylated LPS, and trinitrophenylated NWSM. This synergistic defect in B-lymphocyte function depended on the presence of the CBA/N xid gene but the critical gene(s) from the C3H strain was not the defective Lps gene (Lpsd). These mice should provide a valuable tool for the elucidation of B-lymphocyte ontogeny, heterogeneity, and function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmed A., Scher I., Sharrow S. O., Smith A. H., Paul W. E., Sachs D. H., Sell K. W. B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens. J Exp Med. 1977 Jan 1;145(1):101–110. doi: 10.1084/jem.145.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chien C. C., Lieberman R., Inman J. K. Preparation of functionalized derivatives of inulin: conjugation of erythrocytes for hemagglutination and plaque-forming cell assays. J Immunol Methods. 1979;26(1):39–46. doi: 10.1016/0022-1759(79)90039-5. [DOI] [PubMed] [Google Scholar]
  3. Ciorbaru R., Adam A., Petit J. F., Lederer E., Bona C., Chedid L. Isolation of mitogenic and adjuvant active fractions from various species of Nocardiae. Infect Immun. 1975 Feb;11(2):257–264. doi: 10.1128/iai.11.2.257-264.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Finkelman F. D., Smith A. H., Scher I., Paul W. E. Abnormal ratio of membrane immunoglobulin classes in mice with an X-linked B-lymphocyte defect. J Exp Med. 1975 Nov 1;142(5):1316–1321. doi: 10.1084/jem.142.5.1316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Huber B., Gershon R. K., Cantor H. Identification of a B-cell surface structure involved in antigen-dependent triggering: absence of this structure on B cells from CBA/N mutant mice. J Exp Med. 1977 Jan 1;145(1):10–20. doi: 10.1084/jem.145.1.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inman J. K. Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates. J Immunol. 1975 Feb;114(2 Pt 1):704–709. [PubMed] [Google Scholar]
  7. Johnson G. R., Metcalf D., Wilson J. W. Development of B-lymphocyte colony-forming cells in foetal mouse tissues. Immunology. 1976 Jun;30(6):907–914. [PMC free article] [PubMed] [Google Scholar]
  8. Kincade P. W. Defective colony formation by B lymphocytes from CBA/N and C3H/HeJ mice. J Exp Med. 1977 Feb 1;145(2):249–263. doi: 10.1084/jem.145.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McGhee J. R., Farrar J. J., Michalek S. M., Mergenhagen S. E., Rosenstreich D. L. Cellular requirements for lipopolysaccharide adjuvanticity. A role for both T lymphocytes and macrophages for in vitro responses to particulate antigens. J Exp Med. 1979 Apr 1;149(4):793–807. doi: 10.1084/jem.149.4.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mond J. J., Scher I., Mosier D. E., Baese M., Paul W. E. T-independent responses in B cell-defective CBA/N mice to Brucella abortus and to trinitrophenyl (TNP) conjugates of Brucella abortus. Eur J Immunol. 1978 Jul;8(7):459–463. doi: 10.1002/eji.1830080703. [DOI] [PubMed] [Google Scholar]
  11. Mosier D. E., Zitron I. M., Mond J. J., Ahmed A., Scher I., Paul W. E. Surface immunoglobulin D as a functional receptor for a subclass of B lymphocytes. Immunol Rev. 1977;37:89–104. doi: 10.1111/j.1600-065x.1977.tb00246.x. [DOI] [PubMed] [Google Scholar]
  12. Scher I., Ahmed A., Strong D. M., Steinberg A. D., Paul W. E. X-linked B-lymphocyte immune defect in CBA/HN mice. I. Studies of the function and composition of spleen cells. J Exp Med. 1975 Apr 1;141(4):788–803. [PMC free article] [PubMed] [Google Scholar]
  13. Subbarao B., Ahmed A., Paul W. E., Scher I., Lieberman R., Mosier D. E. Lyb-7, a new B cell alloantigen controlled by genes linked to the IgCH locus. J Immunol. 1979 Jun;122(6):2279–2285. [PubMed] [Google Scholar]
  14. Watson J., Kelly K., Largen M., Taylor B. A. The genetic mapping of a defective LPS response gene in C3H/HeJ mice. J Immunol. 1978 Feb;120(2):422–424. [PubMed] [Google Scholar]

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