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. 1980 Feb 1;151(2):400–406. doi: 10.1084/jem.151.2.400

Isolation and partial characterization of the human homologue of Thy-1

PMCID: PMC2185777  PMID: 6153212

Abstract

A glycoprotein of 25,000 daltons was isolated from a human T lymphoblastoid cell line (MOLT-3). A monovalent antiserum raised against this antigen precipitated mouse Thy-1.1 antigen as well as a 25,000-dalton antigen from human blood T cells. The unabsorbed serum was also cytotoxic for both mouse and human T cells. By multiple criteria, this human T antigen appears to be homologous with mouse Thy- 1.

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Selected References

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  1. Ades E. W., Bukacek A., Zwerner R. K., Dougherty P. A., Balch C. M. Expression of two differentiation antigens on normal and cultured human T cells. J Immunol. 1978 Aug;121(2):513–519. [PubMed] [Google Scholar]
  2. Arndt R., Stark R., Klein P., Müller A., Thiele H. G. Solubilization and molecular characterization of membrane-bound antigens shared by thymocytes and brain. Eur J Immunol. 1976 May;6(5):333–340. doi: 10.1002/eji.1830060506. [DOI] [PubMed] [Google Scholar]
  3. Balch C. M., Ades E. W. Heterogeneity of cell surface xenoantigens on human T lymphocytes. J Reticuloendothel Soc. 1979 Jun;25(6):635–651. [PubMed] [Google Scholar]
  4. Balch C. M., Dagg M. K., Cooper M. D. Cross-reactive T-cell antigens among mammalian species. J Immunol. 1976 Aug;117(2):447–449. [PubMed] [Google Scholar]
  5. Barclay A. N., Letarte-Muirhead M., Williams A. F. Purification of the Thy-1 molecule from rat brain. Biochem J. 1975 Dec;151(3):699–706. doi: 10.1042/bj1510699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dalchau R., Fabre J. W. Identification and unusual tissue distribution of the canine and human homologues of Thy-1 (theta). J Exp Med. 1979 Mar 1;149(3):576–591. doi: 10.1084/jem.149.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Douglas T. C. Occurrence of a theta-like antigen in rats. J Exp Med. 1972 Nov 1;136(5):1054–1062. doi: 10.1084/jem.136.5.1054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hood L., Prahl J. The immune system: a model for differentiation in higher organisms. Adv Immunol. 1971;14:291–351. doi: 10.1016/s0065-2776(08)60287-4. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Letarte-Muirhead M., Barclay A. N., Williams A. F. Purification of the Thy-1 molecule, a major cell-surface glycoprotein of rat thymocytes. Biochem J. 1975 Dec;151(3):685–697. doi: 10.1042/bj1510685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Letarte M., Meghji G. Purification and characterization of mouse brain Thy-1 glycoprotein antigens. J Immunol. 1978 Nov;121(5):1718–1725. [PubMed] [Google Scholar]
  13. McClain L. D., Tomana M., Acton R. T. Purification and characterization of mouse brain Thy-1.2 differentiation alloantigen. Brain Res. 1978 Dec 22;159(1):161–171. doi: 10.1016/0006-8993(78)90117-8. [DOI] [PubMed] [Google Scholar]
  14. Neoh S. H., Jahoda D. M., Rowe D. S., Voller A. Immunoglobulin classes in mammalian species identifed by cross-reactivity with antisera to human immunoglobulin. Immunochemistry. 1973 Dec;10(12):805–813. doi: 10.1016/0019-2791(73)90184-5. [DOI] [PubMed] [Google Scholar]
  15. Owen F. L., Fanger M. W. Studies on the human T-lymphocyte population - IV. The isolation of T-lymphocyte antigens from peripheral lymphocytes. Immunochemistry. 1976 Feb;13(2):121–127. doi: 10.1016/0019-2791(76)90279-2. [DOI] [PubMed] [Google Scholar]
  16. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reif A. E., Allen J. M. Mouse thymic iso-antigens. Nature. 1966 Jan 29;209(5022):521–523. doi: 10.1038/209521b0. [DOI] [PubMed] [Google Scholar]
  18. Stern P. L. Theta alloantigen on mouse and rat fibroblasts. Nat New Biol. 1973 Nov 21;246(151):76–78. doi: 10.1038/newbio246076a0. [DOI] [PubMed] [Google Scholar]
  19. Vaerman J. P., Kobayashi K., Heremans J. F. Precipitin cross-reactions between human and animal J-chains. Adv Exp Med Biol. 1974;45(0):251–255. doi: 10.1007/978-1-4613-4550-3_30. [DOI] [PubMed] [Google Scholar]
  20. Zwerner R. K., Barstad P. A., Acton R. T. Isolation and characterizaiton of murine cell surface components. I. Purification of milligram quantities of Thy-1.1. J Exp Med. 1977 Oct 1;146(4):986–1000. doi: 10.1084/jem.146.4.986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zwerner R. K., Wise K. S., Action R. T. Harvesting the products of cell growth. Methods Enzymol. 1979;58:221–229. doi: 10.1016/s0076-6879(79)58139-7. [DOI] [PubMed] [Google Scholar]

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