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. 1980 Mar 1;151(3):681–694. doi: 10.1084/jem.151.3.681

Feedback suppression of the immune response in vitro. II. IgVH- restricted antibody-dependent suppression

PMCID: PMC2185808  PMID: 6444664

Abstract

Feedback suppression of the primary humoral immune response to sheep erythrocytes (SRBC) in vitro was induced with cell-free supernate material derived from antigen-(SRBC) activated B (sIg+) cells. This soluble products bears Ig determinants and binds to the eliciting antigen (SRBC). The activity of this antibody in suppressing anti-SRBC plaque-forming cell responses is restricted to spleen cell cultures containing B cells sharing VH genes with the B cells producing the suppressive antibody. The anti-hapten (trinitrophenyl) response to derivatized SRBC is not affected by antigen-primed B cells or their products. These data are compatible with suppression being mediated by anti-antigen antibody, either (a) via blockade of different SRBC epitopes recognized by a limited set of B cell clones in each mouse strain, (b) via triggering of an anti-idiotypic response, either antibody or suppressor T cell in nature, restricted to activity in cultures containing B cells sharing VH structures with the original antibody, or (c) via interference by preformed antibody with T cell help directed at idiotype bearing B cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bankert R. B., Pressman D. Receptor-blocking factor present in immune serum resembling auto-anti-idiotype antibody. J Immunol. 1976 Aug;117(2):457–462. [PubMed] [Google Scholar]
  2. Binz H., Wigzell H. Antigen-binding, idiotypic T-lymphocyte receptors. Contemp Top Immunobiol. 1977;7:113–177. doi: 10.1007/978-1-4684-3054-7_4. [DOI] [PubMed] [Google Scholar]
  3. Blomberg B., Geckeler W. R., Weigert M. Genetics of the antibody response to dextran in mice. Science. 1972 Jul 14;177(4044):178–180. doi: 10.1126/science.177.4044.178. [DOI] [PubMed] [Google Scholar]
  4. Bosma M. J., Bosma G. C. Prevention of IgG2a production as a result of allotype-specific interaction between T and B cells. J Exp Med. 1977 Mar 1;145(3):743–748. doi: 10.1084/jem.145.3.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cantor H., Hugenberger J., McVay-Boudreau L., Eardley D. D., Kemp J., Shen F. W., Gershon R. K. Immunoregulatory circuits among T-cell sets. Identification of a subpopulation of T-helper cells that induces feedback inhibition. J Exp Med. 1978 Oct 1;148(4):871–877. doi: 10.1084/jem.148.4.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eardley D. D., Gershon R. K. Induction of specific suppressor T cells in vitro. J Immunol. 1976 Jul;117(1):313–318. [PubMed] [Google Scholar]
  7. Eardley D. D., Hugenberger J., McVay-Boudreau L., Shen F. W., Gershon R. K., Cantor H. Immunoregulatory circuits among T-cell sets. I. T-helper cells induce other T-cell sets to exert feedback inhibition. J Exp Med. 1978 Apr 1;147(4):1106–1115. doi: 10.1084/jem.147.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eardley D. D., Shen F. W., Cantor H., Gershon R. K. Genetic control of immunoregulatory circuits. Genes linked to the Ig locus govern communication between regulatory T-cell sets. J Exp Med. 1979 Jul 1;150(1):44–50. doi: 10.1084/jem.150.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eichmann K. Idiotype expression and the inheritance of mouse antibody clones. J Exp Med. 1973 Mar 1;137(3):603–621. doi: 10.1084/jem.137.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feldmann M., Diener E. Antibody-mediated suppression of the immune response in vitro. I. Evidence for a central effect. J Exp Med. 1970 Feb;131(2):247–274. doi: 10.1084/jem.131.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fitch F. W. Selective suppression of immune responses. Regulation of antibody formation and cell-mediated immunity by antibody. Prog Allergy. 1975;19:195–244. [PubMed] [Google Scholar]
  12. Goidl E. A., Schrater A. F., Siskind G. W., Thorbecke G. J. Production of auto-anti-idiotypic antibody during the normal immune response to TNP-Ficoll. II. Hapten-reversible inhibition of anti-TNP plaque-forming cells by immune serum as an assay for auto-anti-idiotypic antibody. J Exp Med. 1979 Jul 1;150(1):154–165. doi: 10.1084/jem.150.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hetzelberger D., Eichmann K. Idiotype suppression. III. Induction of unresponsiveness to sensitization with anti-idiotypic antibody: identification of the cell types tolerized in high zone and in low zone, suppressor cell-mediated, idiotype suppression. Eur J Immunol. 1978 Dec;8(12):839–846. doi: 10.1002/eji.1830081204. [DOI] [PubMed] [Google Scholar]
  14. Hetzelberger D., Eichmann K. Recognition of idiotypes in lymphocyte interactions. I. Idiotypic selectivity in the cooperation between T and B lymphocytes. Eur J Immunol. 1978 Dec;8(12):846–852. doi: 10.1002/eji.1830081205. [DOI] [PubMed] [Google Scholar]
  15. Kagnoff M. F. Effects of antigen-feeding on intestinal and systemic immune responses. III. Antigen-specific serum-mediated suppression of humoral antibody responses after antigen feeding. Cell Immunol. 1978 Sep 15;40(1):186–203. doi: 10.1016/0008-8749(78)90326-x. [DOI] [PubMed] [Google Scholar]
  16. Kim B. S. Mechanisms of idiotype suppression. I. In vitro generation of idiotype-specific suppressor T cells by anti-idiotype antibodies and specific antigen. J Exp Med. 1979 Jun 1;149(6):1371–1378. doi: 10.1084/jem.149.6.1371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lieberman R., Potter M., Humphrey W., Jr, Chien C. C. Idiotype of inulin-binding antibodies and myeloma proteins controlled by genes linked to the allotype locus of the mouse. J Immunol. 1976 Dec;117(6):2105–2111. [PubMed] [Google Scholar]
  18. Mage M. G., McHugh L. L., Rothstein T. L. Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J Immunol Methods. 1977;15(1):47–56. doi: 10.1016/0022-1759(77)90016-3. [DOI] [PubMed] [Google Scholar]
  19. McCarthy M. M., Dutton R. W. The humoral response of mouse spleen cells to two types of sheep erythrocytes. I. Genetic control of the response to H and L SRBC. J Immunol. 1975 Nov;115(5):1316–1321. [PubMed] [Google Scholar]
  20. McCarthy M. M., Dutton R. W. The humoral response of mouse spleen cells to two types of sheep erythrocytes. III. A new VH region marker. J Immunol. 1975 Nov;115(5):1327–1329. [PubMed] [Google Scholar]
  21. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Owen F. L., Ju S. T., Nisonoff A. Presence on idiotype-specific suppressor T cells of receptors that interact with molecules bearing the idiotype. J Exp Med. 1977 Jun 1;145(6):1559–1566. doi: 10.1084/jem.145.6.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pierce C. W. Immune responses in vitro. II. Suppression of the immune response in vitro by specific antibody. J Exp Med. 1969 Aug 1;130(2):365–379. doi: 10.1084/jem.130.2.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Radcliffe G. N., Axelrad M. A. Immunological memory in vitro. J Exp Med. 1971 Apr 1;133(4):846–856. doi: 10.1084/jem.133.4.846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rajewsky K., Eichmann K. Antigen receptors of T helper cells. Contemp Top Immunobiol. 1977;7:69–112. doi: 10.1007/978-1-4684-3054-7_3. [DOI] [PubMed] [Google Scholar]
  26. Ward K., Cantor H., Nisonoff A. Analysis of the cellular basis of idiotype-specific suppression. J Immunol. 1978 Jun;120(6):2016–2019. [PubMed] [Google Scholar]
  27. Warren R. W., Davie J. M. Antigen mediation of a late-acting suppressor T-cell activity. J Exp Med. 1977 Dec 1;146(6):1627–1639. doi: 10.1084/jem.146.6.1627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wason W. M. Regulation of the immune response with antigen specific IgM antibody: a dual role. J Immunol. 1973 May;110(5):1245–1252. [PubMed] [Google Scholar]
  29. Weinberger J. Z., Greene M. I., Benacerraf B., Dorf M. E. Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. I. Genetic control of delayed-type hypersensitivity by VH and I-A-region genes. J Exp Med. 1979 Jun 1;149(6):1336–1348. doi: 10.1084/jem.149.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zubler R. H., Cantor H., Benacerraf B., Germain R. N. Feedback suppression of the immune response in vitro. I. Activity of antigen-stimulated B cells. J Exp Med. 1980 Mar 1;151(3):667–680. doi: 10.1084/jem.151.3.667. [DOI] [PMC free article] [PubMed] [Google Scholar]

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