Abstract
The intercellular interactions and the site of the genetic defect in delayed-type hypersensitivity (DTH) response to poly(LTyr,LGlu)- poly(DLAla)--poly(LLys) [(T,G)-A--L] has been studied in a system where the T-cell education phase was separated from the efferent phase. In the cellular response, T-T-cell collaboration is required, because T cell-depleted mice were unable to manifest DTH responses after they were transferred with educated and irradiated T cells. Reconstitution of adult thymectomized mice that were irradiated and supplemented with bone marrow cells after treatment with anti-Thy-1.2 serum and complement, with T cells but not with accessory cells gave rise to significant responses. Educated, radioresistant cells required the presence of normal radiosensitive T cells for successful DTH responses to (T,G)-A--L. The genetic defect of nonresponder H-2k and H-2a mice has been located in the above-mentioned, second T-cell population that participates in the efferent phase of this immune reaction. Further characterization revealed that the educated cells are of the Lyt1+ phenotype and that the second normal T cells are expressing the Lyt 1+,2+,3+ phenotype. Thus, the genetic defect of H-2k and H-2a mice in the DTH response to (T,G)-A--L is expressed on the non-antigen- stimulated Lyt 1+,2+,3+ T cells.
Full Text
The Full Text of this article is available as a PDF (507.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eardley D. D., Hugenberger J., McVay-Boudreau L., Shen F. W., Gershon R. K., Cantor H. Immunoregulatory circuits among T-cell sets. I. T-helper cells induce other T-cell sets to exert feedback inhibition. J Exp Med. 1978 Apr 1;147(4):1106–1115. doi: 10.1084/jem.147.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eshhar Z., Strassmann G., Waks T., Mozes E. In vitro and in vivo induction of effector T cells mediating DTH responses to a protein and a synthetic polypeptide antigen. Cell Immunol. 1979 Oct;47(2):378–389. doi: 10.1016/0008-8749(79)90347-2. [DOI] [PubMed] [Google Scholar]
- Feldmann M., Beverley P. C., Woody J., McKenzie I. F. T-T interactions in the induction of suppressor and helper T cells: analysis of membrane phenotype of precursor and amplifier cells. J Exp Med. 1977 Apr 1;145(4):793–801. doi: 10.1084/jem.145.4.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glasser M., Law L. W. T-T cell collaboration in rejection of a syngeneic SV40-induced sarcoma in mice. Nature. 1978 Jun 1;273(5661):385–387. doi: 10.1038/273385a0. [DOI] [PubMed] [Google Scholar]
- Huber B., Devinsky O., Gershon R. K., Cantor H. Cell-mediated immunity: delayed-type hypersensitivity and cytotoxic responses are mediated by different T-cell subclasses. J Exp Med. 1976 Jun 1;143(6):1534–1539. doi: 10.1084/jem.143.6.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
- McDevitt H. O., Sela M. Genetic control of the antibody response. I. Demonstration of determinant-specific differences in response to synthetic polypeptide antigens in two strains of inbred mice. J Exp Med. 1965 Sep 1;122(3):517–531. doi: 10.1084/jem.122.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller J. F., Vadas M. A., Whitelaw A., Gamble J., Bernard C. Histocompatibility linked immune responsiveness and restrictions imposed on sensitized lymphocytes. J Exp Med. 1977 Jun 1;145(6):1623–1628. doi: 10.1084/jem.145.6.1623. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SELA M., FUCHS S., ARNON R. Studies on the chemical basis of the antigenicity of proteins. 5. Synthesis, characterization and immunogenicity of some multichain and linear polypeptides containing tyrosine. Biochem J. 1962 Oct;85:223–235. doi: 10.1042/bj0850223. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwartz R. H., Horton C. L., Paul W. E. T-lymphocyte-enriched murine peritoneal exudate cells. IV. Genetic control of cross-stimulation at the T-cell level. J Exp Med. 1977 Feb 1;145(2):327–343. doi: 10.1084/jem.145.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sela M. Antigenicity: some molecular aspects. Science. 1969 Dec 12;166(3911):1365–1374. doi: 10.1126/science.166.3911.1365. [DOI] [PubMed] [Google Scholar]
- Strassmann G., Eshhar Z., Mozes E. Genetic regulation of delayed-type hypersensitivity responses to poly(LTyr,LGu)-poly(DLAla)--poly(LLys). I. Expression of the genetic defect at two phases of the immune process. J Exp Med. 1980 Feb 1;151(2):265–274. doi: 10.1084/jem.151.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stutman O., Shen F. W., Boyse E. A. Ly phenotype of T cells cytotoxic for syngeneic mouse mammary tumors: evidence for T cell interactions. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5667–5671. doi: 10.1073/pnas.74.12.5667. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tada T., Takemori T., Okumura K., Nonaka M., Tokuhisa T. Two distinct types of helper T cells involved in the secondary antibody response: independent and synergistic effects of Ia- and Ia+ helper T cells. J Exp Med. 1978 Feb 1;147(2):446–458. doi: 10.1084/jem.147.2.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vadas M. A., Miller J. F., Gamble J., Whitelaw A. A radioisotopic method to measure delayed type hypersensitivity in the mouse. I. Studies in sensitized and normal mice. Int Arch Allergy Appl Immunol. 1975;49(5):670–692. doi: 10.1159/000231449. [DOI] [PubMed] [Google Scholar]