Abstract
The neutral lipid fraction of the photochromogenic, coryneform bacterium Cellulomonas dehydrogenans (Arnaudi 1942) comb. nov. contains the sterol precursor squalene and at least two sterols, cholesterol and beta-sitosterol. The compounds were characterized by mass spectrometry and combination gas-liquid chromatography--mass spectrometry. De novo sterol biosynthetic ability was shown from incorporation of 14C from D-[U-14C]glucose into squalene and the sterol fraction. The squalene concentration approximated 0.002 to 0.005% of the total dry cell weight, and the sterols approximated 0.03 to 0.05%.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Bird C. W., Lynch J. M., Pirt F. J., Reid W. W. Steroids and squalene in Methylococcus capsulatus grown on methane. Nature. 1971 Apr 16;230(5294):473–474. doi: 10.1038/230473a0. [DOI] [PubMed] [Google Scholar]
- CARROLL K. K. Quantitative estimation of peak areas in gas-liquid chromatography. Nature. 1961 Jul 22;191:377–378. doi: 10.1038/191377a0. [DOI] [PubMed] [Google Scholar]
- Goad L. J. Sterol biosynthesis. Biochem Soc Symp. 1970;29:45–77. [PubMed] [Google Scholar]
- Kramer J. K., Kushwaha S. C., Kates M. Structure ditermination of the squalene, dihydrosqualene and tetrahydrosqualene in Halobacterium cutirubrum. Biochim Biophys Acta. 1972 May 23;270(1):103–110. doi: 10.1016/0005-2760(72)90183-x. [DOI] [PubMed] [Google Scholar]
- Lepage M. Identification and composition of turnip root lipids. Lipids. 1967 May;2(3):244–250. doi: 10.1007/BF02532563. [DOI] [PubMed] [Google Scholar]
- Liaaen-Jensen S., Hertzberg S., Weeks O. B., Schwieter U. Bacterial carotenoids XXVII. C50-carotenoids. 3. Structure determination of dehydrogenans-P439. Acta Chem Scand. 1968;22(4):1171–1186. doi: 10.3891/acta.chem.scand.22-1171. [DOI] [PubMed] [Google Scholar]
- Schubert K., Rose G., Tümmler R., Ikekawa N. Sterine in Escherichia coli. Hoppe Seylers Z Physiol Chem. 1964;339(1):293–296. [PubMed] [Google Scholar]
- Schubert K., Rose G., Wachtel H., Hörhold C., Ikekawa N. Zum Vorkommen von Sterinen in Bakterien. Eur J Biochem. 1968 Jul;5(2):246–251. doi: 10.1111/j.1432-1033.1968.tb00364.x. [DOI] [PubMed] [Google Scholar]
- Suzue G., Tsukada K., Tanaka S. A new triterpenoid from a mutant of Staphylococcus aureus. Biochim Biophys Acta. 1967 Aug 8;144(1):186–188. [PubMed] [Google Scholar]
- Tornabene T. G., Kates M., Gelpi E., Oro J. Occurrence of squalene, di- and tetrahydrosqualenes, and vitamin MK8 in an extremely halophilic bacterium, Halobacterium cutirubrun. J Lipid Res. 1969 May;10(3):294–303. [PubMed] [Google Scholar]
- Weeks O. B., Andrewes A. G., Brown B. O., Weedon B. C. Occurrence of C40 and C45 carotenoids in the C50 carotenoid system of Flavobacterium dehydrogenans. Nature. 1969 Nov 29;224(5222):879–882. doi: 10.1038/224879a0. [DOI] [PubMed] [Google Scholar]
- Weeks O. B., Garner R. J. Biosynthesis of carotenoids in Flavobacterium dehydrogenans Arnaudi. Arch Biochem Biophys. 1967 Jul;121(1):35–49. doi: 10.1016/0003-9861(67)90007-0. [DOI] [PubMed] [Google Scholar]
- Weeks O. B., Saleh F. K., Wirahadikusumah M., Berry R. A. Photoregulated carotenoid biosynthesis in non-photosynthetic microorganisms. Pure Appl Chem. 1973;35(1):63–80. doi: 10.1351/pac197335010063. [DOI] [PubMed] [Google Scholar]