Abstract
The antibody response to the inulin [(In), beta-(2 leads to 1) fructosan] determinant of bacterial levan [(BL), a beta-(2 leads to 6) polyfructosan that contains beta-(2 leads to 1) branch points] requires the presence of the a haplotype of the Igh gene complex. BALB/c (Igh a) mice immunized with BL produce IgG anti-In antibodies of a single spectrotype by isoelectric focusing analysis. C57BL/6 mice, which possess the b haplotype of the Igh gene complex and which fail to produce anti-In antibodies, nevertheless possess a gene, spectrotype regulation gene 1 (Sr-1), that regulates the isoelectric focusing (IEF) pattern of anti-In antibodies in mice of the a haplotype. Thus, the IEF patterns of anti-In antibodies of (BALB/c x C57BL/6)F1 mice and of B.C8 mice (C57BL/Ka . Igh-Ca) are considerably more complex than those of BALB/c. Backcross analysis indicates that Sr-1 is not linked to the Igh complex, the major histocompatibility complex, or to the genes that code for coat color. Studies of the heterogeneity of anti-In antibodies in recombinant inbred lines and their progeny from matings to BALB/c and C.B20 (BALB/c . Igh-Cb) suggest the existence of other regulatory genes.
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Selected References
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- Briles D. E., Davie J. M. Detection of isoelectric focused antibody by autoradiography and hemolysis of antigen-coated erythrocytes. A comparison of methods. J Immunol Methods. 1975 Oct;8(4):363–372. doi: 10.1016/0022-1759(75)90058-7. [DOI] [PubMed] [Google Scholar]
- Chien C. C., Lieberman R., Inman J. K. Preparation of functionalized derivatives of inulin: conjugation of erythrocytes for hemagglutination and plaque-forming cell assays. J Immunol Methods. 1979;26(1):39–46. doi: 10.1016/0022-1759(79)90039-5. [DOI] [PubMed] [Google Scholar]
- Eichmann K. Idiotype expression and the inheritance of mouse antibody clones. J Exp Med. 1973 Mar 1;137(3):603–621. doi: 10.1084/jem.137.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keck K. An easy method for labelling polysaccharides with radioactive iodine. Immunochemistry. 1972 Mar;9(3):359–360. doi: 10.1016/0019-2791(72)90098-5. [DOI] [PubMed] [Google Scholar]
- Kunkel H. G., Mannik M., Williams R. C. Individual Antigenic Specificity of Isolated Antibodies. Science. 1963 Jun 14;140(3572):1218–1219. doi: 10.1126/science.140.3572.1218. [DOI] [PubMed] [Google Scholar]
- Lieberman R., Bona C., Chien C. C., Stein K. E., Paul W. E. Genetic and cellular regulation of the expression of specific antibody idiotypes in the anti-polyfructosan immune response. Ann Immunol (Paris) 1979 Mar-Apr;130(2):247–262. [PubMed] [Google Scholar]
- Lieberman R., Potter M., Humphrey W., Jr, Chien C. C. Idiotype of inulin-binding antibodies and myeloma proteins controlled by genes linked to the allotype locus of the mouse. J Immunol. 1976 Dec;117(6):2105–2111. [PubMed] [Google Scholar]
- Lieberman R., Potter M., Humphrey W., Jr, Mushinski E. B., Vrana M. Multiple individual and cross-specific indiotypes on 13 levan-binding myeloma proteins of BALB/c mice. J Exp Med. 1975 Jul 1;142(1):106–119. doi: 10.1084/jem.142.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perlmutter R. M., Hansburg D., Briles D. E., Nicolotti R. A., Davie J. M. Subclass restriction of murine anti-carbohydrate antibodies. J Immunol. 1978 Aug;121(2):566–572. [PubMed] [Google Scholar]
- Riblet R., Blomberg B., Weigert M., Lieberman R., Taylor B. A., Potter M. Genetics of mouse antibodies. I. Linkage of the dextran response locus, VH-DEX, to allotype. Eur J Immunol. 1975 Nov;5(11):775–777. doi: 10.1002/eji.1830051109. [DOI] [PubMed] [Google Scholar]
- Sachs D. H., Winn H. J., Russell P. S. The immunologic response to xenografts. Recognition of mouse H-2 histocompatibility antigens by the rat. J Immunol. 1971 Aug;107(2):481–492. [PubMed] [Google Scholar]
- Slack J. H., Shapiro M., Potter M. Serum expression of a VK structure, VK-11, associated with inulin antibodies controlled by gene(s) linked to the mouse IgCH complex. J Immunol. 1979 Jan;122(1):230–239. [PubMed] [Google Scholar]