Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1980 Jun 1;151(6):1386–1397. doi: 10.1084/jem.151.6.1386

Dissection of the B10.D2 anti-H-2Kb cytolytic T lymphocyte receptor repertoire

PMCID: PMC2185868  PMID: 6155421

Abstract

The B10.D2 cytolytic T lymphocyte (CTL) receptor repertoire specific for the H-2Kb alloantigen has been studied by determining the reactivity patterns of monoclonal CTL against a panel of seven different H-2Kb mutants. The repertoire is extremely diverse and contains a minimum of approximately 50 different specificities against unique antigenic determinants on the H-2Kb molecule. Each specificity appears at a maximum frequency of 1 in 18,000 CTL precursors. These studies have also served to dissect the antigenic composition of the H- 2Kb molecule. Very few CTL clonotypes share recognition of all the mutants, thereby indicating the lack of conservation of a b-type antigenic region. In addition, the degree to which each mutant shares antigenic determinants with the standard H-2Kb molecule has been determined.

Full Text

The Full Text of this article is available as a PDF (723.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach B. A., Sherman L., Benacerraf B., Greene M. I. Mechanisms of regulation of cell-mediated immunity. II. Induction and suppression of delayed-type hypersensitivity to azobenzenearsonate-coupled syngeneic cells. J Immunol. 1978 Oct;121(4):1460–1468. [PubMed] [Google Scholar]
  2. Bach F. H., Bock H., Graupner K., Day E., Klostermann H. Cell kinetic studies in mixed leukocyte cultures: an in vitro model of homograft reactivity. Proc Natl Acad Sci U S A. 1969 Feb;62(2):377–384. doi: 10.1073/pnas.62.2.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker P. E., Gillis S., Smith K. A. Monoclonal cytolytic T-cell lines. J Exp Med. 1979 Jan 1;149(1):273–278. doi: 10.1084/jem.149.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bevan M. J., Fink P. J. The influence of thymus H-2 antigens on the specificity of maturing killer and helper cells. Immunol Rev. 1978;42:3–19. doi: 10.1111/j.1600-065x.1978.tb00256.x. [DOI] [PubMed] [Google Scholar]
  5. Bevan M. J., Langman R. E., Cohn M. H-2 antigen-specific cytotoxic T cells induced by concanavalin A: estimation of their relative frequency. Eur J Immunol. 1976 Mar;6(3):150–156. doi: 10.1002/eji.1830060303. [DOI] [PubMed] [Google Scholar]
  6. Binz H., Frischknecht H., Shen F. W., Wigzell H. Idiotypic determinants on T-cell subpopulations. J Exp Med. 1979 Apr 1;149(4):910–922. doi: 10.1084/jem.149.4.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Binz H., Wigzell H. Antigen-binding, idiotypic T-lymphocyte receptors. Contemp Top Immunobiol. 1977;7:113–177. doi: 10.1007/978-1-4684-3054-7_4. [DOI] [PubMed] [Google Scholar]
  8. Binz H., Wigzell H. Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens. J Exp Med. 1975 Jul 1;142(1):197–211. doi: 10.1084/jem.142.1.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Brondz B. D., Andreev A. V., Egorova S. G., Drizlikh G. I. Cross-reactivity of cytotoxic T-lymphocyte antigen-binding receptors immune to antigens of the H-2 system. Scand J Immunol. 1979;10(3):195–205. doi: 10.1111/j.1365-3083.1979.tb01340.x. [DOI] [PubMed] [Google Scholar]
  10. Cancro M. P., Gerhard W., Klinman N. R. The diversity of the influenza-specific primary B-cell repertoire in BALB/c mice. J Exp Med. 1978 Mar 1;147(3):776–787. doi: 10.1084/jem.147.3.776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cosenza H., Köhler H. Specific inhibition of plaque formation to phosphorylcholine by antibody against antibody. Science. 1972 Jun 2;176(4038):1027–1029. doi: 10.1126/science.176.4038.1027. [DOI] [PubMed] [Google Scholar]
  12. Eichmann K., Rajewsky K. Induction of T and B cell immunity by anti-idiotypic antibody. Eur J Immunol. 1975 Oct;5(10):661–666. doi: 10.1002/eji.1830051002. [DOI] [PubMed] [Google Scholar]
  13. Ford W. L., Simmonds S. J., Atkins R. C. Early cellular events in a systemic graft-vs.-host reaction. II. Autoradiographic estimates of the frequency of donor lymphocytes which respond to each Ag-B-determined antigenic complex. J Exp Med. 1975 Mar 1;141(3):681–696. doi: 10.1084/jem.141.3.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Forman J. The specificity of thymus-derived T-cells in cell-mediated cytotoxic reactions. Transplant Rev. 1976;29:146–163. doi: 10.1111/j.1600-065x.1976.tb00200.x. [DOI] [PubMed] [Google Scholar]
  15. Gerhard W. The analysis of the monoclonal immune response to influenza virus. II. The antigenicity of the viral hemagglutinin. J Exp Med. 1976 Oct 1;144(4):985–995. doi: 10.1084/jem.144.4.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  17. Janeway C. A., Jr The specificity of T lymphocyte responses to chemically defined antigens. Transplant Rev. 1976;29:164–188. doi: 10.1111/j.1600-065x.1976.tb00201.x. [DOI] [PubMed] [Google Scholar]
  18. Julius M. H., Cosenza H., Augustin A. A. Parallel expression of new idiotypes on T and B cells. Nature. 1977 Jun 2;267(5610):437–439. doi: 10.1038/267437a0. [DOI] [PubMed] [Google Scholar]
  19. Katz D. H., Hamaoka T., Benacerraf B. Cell interactions between histoincompatible T and B lymphocytes. II. Failure of physiologic cooperative interactions between T and B lymphocytes from allogeneic donor strains in humoral response to hapten-protein conjugates. J Exp Med. 1973 Jun 1;137(6):1405–1418. doi: 10.1084/jem.137.6.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Klein J. H-2 mutations: their genetics and effect on immune functions. Adv Immunol. 1978;26:55–146. doi: 10.1016/s0065-2776(08)60229-1. [DOI] [PubMed] [Google Scholar]
  21. Krawinkel U., Cramer M., Imanishi-Kari T., Jack R. S., Rajewsky K., Mäkelä O. Isolated hapten-binding receptors of sensitized lymphocytes. I. Receptors from nylon wool-enriched mouse T lymphocytes lack serological markers of immunoglobulin constant domains but express heavy chain variable portions. Eur J Immunol. 1977 Aug;7(8):566–573. doi: 10.1002/eji.1830070814. [DOI] [PubMed] [Google Scholar]
  22. Lindahl K. F., Wilson D. B. Histocompatibility antigen-activated cytotoxic T lymphocytes. II. Estimates of the frequency and specificity of precursors. J Exp Med. 1977 Mar 1;145(3):508–522. doi: 10.1084/jem.145.3.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Miller J. F., Vadas M. A., Whitelaw A., Gamble J. H-2 gene complex restricts transfer of delayed-type hypersensitivity in mice. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5095–5098. doi: 10.1073/pnas.72.12.5095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nabholz M., Engers H. D., Collavo D., North M. Cloned T-cell lines with specific cytolytic activity. Curr Top Microbiol Immunol. 1978;81:176–187. doi: 10.1007/978-3-642-67448-8_29. [DOI] [PubMed] [Google Scholar]
  25. Paul W. E. Functional specificity of antigen-binding receptors of lymphocytes. Transplant Rev. 1970;5:130–166. doi: 10.1111/j.1600-065x.1970.tb00359.x. [DOI] [PubMed] [Google Scholar]
  26. Pimsler M., Forman J. Estimates of the precursor frequency of cytotoxic T lymhocytes against antigens controlled by defined regions of the H-2 gene complex: comparison of the effect of H-2 differences due to intra-H-2 recombination vs mutation. J Immunol. 1978 Oct;121(4):1302–1305. [PubMed] [Google Scholar]
  27. Ramseier H., Lindenmann J. Aliotypic antibodies. Transplant Rev. 1972;10:57–96. doi: 10.1111/j.1600-065x.1972.tb01539.x. [DOI] [PubMed] [Google Scholar]
  28. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shearer G. M. Cell-mediated cytotoxicity to trinitrophenyl-modified syngeneic lymphocytes. Eur J Immunol. 1974 Aug;4(8):527–533. doi: 10.1002/eji.1830040802. [DOI] [PubMed] [Google Scholar]
  30. Sigal N. H., Klinman N. R. The B-cell clonotype repertoire. Adv Immunol. 1978;26:255–337. doi: 10.1016/s0065-2776(08)60232-1. [DOI] [PubMed] [Google Scholar]
  31. Sprent J. Role of H-2 gene products in the function of T helper cells from normal and chimeric mice in vivo. Immunol Rev. 1978;42:108–137. doi: 10.1111/j.1600-065x.1978.tb00260.x. [DOI] [PubMed] [Google Scholar]
  32. Teh H. S., Harley E., Phillips R. A., Miller R. G. Quantitative studies on the precursors of cytotoxic lymphocytes. I. Characterization of a clonal assay and determination of the size of clones derived from single precursors. J Immunol. 1977 Mar;118(3):1049–1056. [PubMed] [Google Scholar]
  33. Weinberger J. Z., Germain R. N., Ju S. T., Greene M. I., Benacerraf B., Dorf M. E. Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. II. Demonstration of idiotypic determinants on suppressor T cells. J Exp Med. 1979 Oct 1;150(4):761–776. doi: 10.1084/jem.150.4.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wilson D. B., Blyth JL NOWELL P. C. Quantitative studies on the mixed lymphocyte interaction in rats. 3. Kinetics of the response. J Exp Med. 1968 Nov 1;128(5):1157–1181. doi: 10.1084/jem.128.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Zinkernagel R. M., Callahan G. N., Althage A., Cooper S., Klein P. A., Klein J. On the thymus in the differentiation of "H-2 self-recognition" by T cells: evidence for dual recognition? J Exp Med. 1978 Mar 1;147(3):882–896. doi: 10.1084/jem.147.3.882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Zinkernagel R. M., Doherty P. C. Restriction of in vitro T cell-mediated cytotoxicity in lymphocytic choriomeningitis within a syngeneic or semiallogeneic system. Nature. 1974 Apr 19;248(5450):701–702. doi: 10.1038/248701a0. [DOI] [PubMed] [Google Scholar]
  37. von Boehmer H., Haas W., Jerne N. K. Major histocompatibility complex-linked immune-responsiveness is acquired by lymphocytes of low-responder mice differentiating in thymus of high-responder mice. Proc Natl Acad Sci U S A. 1978 May;75(5):2439–2442. doi: 10.1073/pnas.75.5.2439. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES