Abstract
Direct microcytotoxicity testing and absorption analyses were employed to determine whether H-2K, H-2D, and Ia antigens are present on murine islet of Langerhans cells. Products of the H-2K and H-2D loci were found on beta-cells from three different mouse strains, but I-region (Ia) antigens were not detected. The absence of Ia gene products from islet cells may be of importance in explaining the survival of islet allografts across major histocompatibility barriers.
Full Text
The Full Text of this article is available as a PDF (352.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abbas A. K., Dorf M. E., Karnovsky M. J., Unanue E. R. The distribution of Ia antigens on the surfaces of lymphocytes. J Immunol. 1976 Feb;116(2):371–378. [PubMed] [Google Scholar]
- Cowing C., Schwartz B. D., Dickler H. B. Macrophage Ia antigens. I. macrophage populations differ in their expression of Ia antigens. J Immunol. 1978 Feb;120(2):378–384. [PubMed] [Google Scholar]
- Curman B., Kämpe O., Rask L., Peterson P. A. Presence of alloreactive Ia antigens on murine intestine epithelial cells. Scand J Immunol. 1979;10(1):11–15. doi: 10.1111/j.1365-3083.1979.tb01328.x. [DOI] [PubMed] [Google Scholar]
- Finke E. H., Lacy P. E., Ono J. Use of reflected green light for specific identification of islets in vitro after collagenase isolation. Diabetes. 1979 Jun;28(6):612–613. doi: 10.2337/diab.28.6.612. [DOI] [PubMed] [Google Scholar]
- GOMORI G. Aldehyde-fuchsin: a new stain for elastic tissue. Am J Clin Pathol. 1950 Jul;20(7):665–666. [PubMed] [Google Scholar]
- Hauptfeld V., Hauptfeld M., Klein J. Tissue distribution of I region-associated antigens in the mouse. J Immunol. 1974 Jul;113(1):181–188. [PubMed] [Google Scholar]
- Lacy P. E., Davie J. M., Finke E. H. Induction of rejection of successful allografts of rat islets by donor peritoneal exudate cells. Transplantation. 1979 Nov;28(5):415–420. doi: 10.1097/00007890-197911000-00014. [DOI] [PubMed] [Google Scholar]
- Lacy P. E., Davie J. M., Finke E. H. Prolongation of islet allograft survival following in vitro culture (24 degrees C) and a single injection of ALS. Science. 1979 Apr 20;204(4390):312–313. doi: 10.1126/science.107588. [DOI] [PubMed] [Google Scholar]
- Lacy P. E., Davie J. M., Finke E. H., Scharp D. W. Prolongation of islet allograft survival. Transplantation. 1979 Mar;27(3):171–174. doi: 10.1097/00007890-197903000-00006. [DOI] [PubMed] [Google Scholar]
- Lacy P. E., Kostianovsky M. Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes. 1967 Jan;16(1):35–39. doi: 10.2337/diab.16.1.35. [DOI] [PubMed] [Google Scholar]
- Lindall A., Steffes M., Sorenson R. Immunoassayable insulin content of subcellular fractions of rat islets. Endocrinology. 1969 Aug;85(2):218–223. doi: 10.1210/endo-85-2-218. [DOI] [PubMed] [Google Scholar]
- Ono J., Takaki R., Fukuma M. Preparation of single cells from pancreatic islets of adult rat by the use of dispase. Endocrinol Jpn. 1977 Jun;24(3):265–270. doi: 10.1507/endocrj1954.24.265. [DOI] [PubMed] [Google Scholar]
- Parr E. L. The absence of H-2 antigens from mouse pancreatic beta-cells demonstrated by immunoferritin labeling. J Exp Med. 1979 Jul 1;150(1):1–9. doi: 10.1084/jem.150.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
- Talmage D. W., Dart G., Radovich J., Lafferty K. J. Activation of transplant immunity: effect of donor leukocytes on thyroid allograft rejection. Science. 1976 Jan 30;191(4225):385–388. doi: 10.1126/science.1082167. [DOI] [PubMed] [Google Scholar]