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. 1978 Nov;136(2):625–630. doi: 10.1128/jb.136.2.625-630.1978

Benzeneboronic acid selectively inhibits sporulation of Bacillis subtilis.

K Davis-Mancini, I P Lopez, J H Hageman
PMCID: PMC218587  PMID: 30755

Abstract

m-Aminobenzeneboronic acid at levels of 0.2 mM in nutrient broth medium selectively inhibited sporulation without appreciably altering vegetative growth. Significant inhibitory effects were seen even when it was added as late as 6 h after the end of logarithmic growth. The pH changes associated with growth and sporulation of Bacillus subtilis in nutrient broth were not significantly altered by the inhibitor. When it was present in cultures of actively growing cells, its inhibitory effect could not be reversed by simple dilution. The compound caused extensive clumping, of cells, which appeared not to be related to the ability of boronates to esterify to diols.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bohin J. P., Rigomier D., Schaeffer P. Ethanol sensitivity of sporulation in Bacillus subtilis: a new tool for the analysis of the sporulation process. J Bacteriol. 1976 Aug;127(2):934–940. doi: 10.1128/jb.127.2.934-940.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyer H. W., Carlton B. C. Production of two proteolytic enzymes by a transformable strain of Bacillus subtilis. Arch Biochem Biophys. 1968 Nov;128(2):442–455. doi: 10.1016/0003-9861(68)90050-7. [DOI] [PubMed] [Google Scholar]
  3. Burke W. F., Jr, Spizizen J. Selective inhibition of Bacillus subtilis sporulation by acridine orange and promethazine. J Bacteriol. 1977 Mar;129(3):1215–1221. doi: 10.1128/jb.129.3.1215-1221.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dancer B. N., Mandelstam J. Production and possible function of serine protease during sporulation of Bacillus subtilis. J Bacteriol. 1975 Feb;121(2):406–410. doi: 10.1128/jb.121.2.406-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deutscher M. P., Kornberg A. Biochemical studies of bacterial sporulation and germination. 8. Patterns of enzyme development during growth and sporulation of Baccillus subtilis. J Biol Chem. 1968 Sep 25;243(18):4653–4660. [PubMed] [Google Scholar]
  6. Doyle R. J., McDannel M. L., Helman J. R., Streips U. N. Distribution of teichoic acid in the cell wall of Bacillus subtilis. J Bacteriol. 1975 Apr;122(1):152–158. doi: 10.1128/jb.122.1.152-158.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fortnagel P., Freese E. Inhibition of aconitase by chelation of transition metals causing inhibition of sporulation in Bacillus subtilis. J Biol Chem. 1968 Oct 25;243(20):5289–5295. [PubMed] [Google Scholar]
  8. GOLLAKOTA K. G., HALVORSON H. O. Biochemical changes occurring during sporulation of Bacillus cereus. Inhibition of sporulation by alpha-picolinic acid. J Bacteriol. 1960 Jan;79:1–8. doi: 10.1128/jb.79.1.1-8.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hageman J. H., Carlton B. C. An enzymatic and immunological comparison of two proteases from a transformable Bacillus subtilis with the "subtilisins". Arch Biochem Biophys. 1970 Jul;139(1):67–79. doi: 10.1016/0003-9861(70)90045-7. [DOI] [PubMed] [Google Scholar]
  10. Hageman J. H., Carlton B. C. Effects of mutational loss of specific intracellular proteases on the sporulation of Bacillus subtilis. J Bacteriol. 1973 May;114(2):612–617. doi: 10.1128/jb.114.2.612-617.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Harmon J. M., Taber H. W. Some properties of a membrane-deoxyribonucleic acid complex isolated from Bacillus subtilis. J Bacteriol. 1977 Feb;129(2):789–795. doi: 10.1128/jb.129.2.789-795.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Keilman G. R., Tanimoto B., Doi R. H. Selective inhibition of sporulation of Bacillus subtilis by netropsin. Biochem Biophys Res Commun. 1975 Nov 3;67(1):414–420. doi: 10.1016/0006-291x(75)90331-9. [DOI] [PubMed] [Google Scholar]
  13. Lindquist R. N., Terry C. Inhibition of subtilisin by boronic acids, potential analogs of tetrahedral reaction intermediates. Arch Biochem Biophys. 1974 Jan;160(1):135–144. doi: 10.1016/s0003-9861(74)80018-4. [DOI] [PubMed] [Google Scholar]
  14. Mamas S., Millet J. Purification et propriétés d'une estérase excrétée pendant la sporulation de Bacillus subtilis. Biochimie. 1975;57(1):9–16. doi: 10.1016/s0300-9084(75)80104-0. [DOI] [PubMed] [Google Scholar]
  15. Maurizi M. R., Switzer R. L. Apparent inactivation of inosine 5'-monophosphate dehydrogenase in sporulating Bacillus subtilis Is an artifact of in vitro proteolysis. J Bacteriol. 1975 Sep;123(3):1269–1272. doi: 10.1128/jb.123.3.1269-1272.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Millet J. Caractérisation de deux endopeptidases excrétées par B. subtilis Marburg au cours de la sporulation. Bull Soc Chim Biol (Paris) 1969 Jul 25;51(3):457–469. [PubMed] [Google Scholar]
  17. Millet J. Characterization of a protein inhibitor of intracellular protease from Bacillus subtilis. FEBS Lett. 1977 Feb 15;74(1):59–61. doi: 10.1016/0014-5793(77)80752-7. [DOI] [PubMed] [Google Scholar]
  18. Millet J. Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation. J Appl Bacteriol. 1970 Mar;33(1):207–219. doi: 10.1111/j.1365-2672.1970.tb05245.x. [DOI] [PubMed] [Google Scholar]
  19. Ramaley R. F., Burden L. Replacement sporulation of Bacillus subtilis 168 in a chemically defined medium. J Bacteriol. 1970 Jan;101(1):1–8. doi: 10.1128/jb.101.1.1-8.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reysset G., Millet J. Characterization of an intracellular protease in B. subtillus during sporulation. Biochem Biophys Res Commun. 1972 Oct 17;49(2):328–334. doi: 10.1016/0006-291x(72)90414-7. [DOI] [PubMed] [Google Scholar]
  21. Rogolsky M., Nakamura H. T. Sensitivity of an early step in the sporulation of Bacillus subtilis to selective inhibition by ethidium bromide. J Bacteriol. 1974 Jul;119(1):57–61. doi: 10.1128/jb.119.1.57-61.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SLEPECKY R. A. INHIBITION OF SPORULATION AND GERMINATION OF BACILLUS MEGATERIUM BY PHENETHYL ALCOHOL. Biochem Biophys Res Commun. 1963 Aug 14;12:369–373. doi: 10.1016/0006-291x(63)90107-4. [DOI] [PubMed] [Google Scholar]
  23. Silva M. T., Sousa J. C., Macedo M. A., Polónia J., Parente A. M. Effects of phenethyl alcohol on Bacillus and Streptococcus. J Bacteriol. 1976 Sep;127(3):1359–1369. doi: 10.1128/jb.127.3.1359-1369.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Smith K. W., Johnson S. L. Borate inhibition of yeast alcohol dehydrogenase. Biochemistry. 1976 Feb 10;15(3):560–565. doi: 10.1021/bi00648a016. [DOI] [PubMed] [Google Scholar]
  25. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stepanov V. M., Strongin A. Y., Izotova L. S., Abramov Z. T., Lyublinskaya L. A., Ermakova L. M., Baratova L. A., Belyanova L. P. Intracellular serine protease from Bacillus subtilis. Structural comparison with extracellular serine proteases-subtilisins. Biochem Biophys Res Commun. 1977 Jul 11;77(1):298–305. doi: 10.1016/s0006-291x(77)80196-4. [DOI] [PubMed] [Google Scholar]
  27. Strongin A. Y., Izotova L. S., Abramov Z. T., Gorodetsky D. I., Ermakova L. M., Baratova L. A., Belyanova L. P., Stepanov V. M. Intracellular serine protease of Bacillus subtilis: sequence homology with extracellular subtilisins. J Bacteriol. 1978 Mar;133(3):1401–1411. doi: 10.1128/jb.133.3.1401-1411.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weith H. L., Wiebers J. L., Gilham P. T. Synthesis of cellulose derivatives containing the dihydroxyboryl group and a study of their capacity to form specific complexes with sugars and nucleic acid components. Biochemistry. 1970 Oct 27;9(22):4396–4401. doi: 10.1021/bi00824a021. [DOI] [PubMed] [Google Scholar]

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