Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1980 Jun 1;151(6):1528–1533. doi: 10.1084/jem.151.6.1528

Different divalent cation requirements for binding IgM complexes to lymphocytes and macrophages

PMCID: PMC2185888  PMID: 7381365

Abstract

We have found that Mg++ supports IgM complex binding to mouse lymphocytes but not to macrophages. In contrast, Ca++ supports IgM complex binding to macrophages but not lymphocytes. IgG complex binding to both lymphocytes and macrophages is divalent cation independent. These findings allow one to distinguish IgG from IgM binding and macrophage from lymphocyte IgM complex binding by their differential divalent cation requirements.

Full Text

The Full Text of this article is available as a PDF (334.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basten A., Miller J. F., Sprent J., Pye J. A receptor for antibody on B lymphocytes. I. Method of detection and functional significance. J Exp Med. 1972 Mar 1;135(3):610–626. doi: 10.1084/jem.135.3.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collisson E. W., Andersson B., Lamon E. W. Modulation of hapten-specific antibody responses with anticarrier antibody: I. Differential effects of IgM and IgG anticarrier on primary direct and indirect hapten-specific plaque-forming cells. Proc Soc Exp Biol Med. 1979 Oct;162(1):194–198. doi: 10.3181/00379727-162-40645. [DOI] [PubMed] [Google Scholar]
  4. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fuson E. W., Lamon E. W. IgM-induced cell-mediated cytotoxicity with antibody and effector cells of human origin. J Immunol. 1977 May;118(5):1907–1909. [PubMed] [Google Scholar]
  6. Fuson E. W., Walia A. S., Cox B. A., Lamon E. W. Antibody-dependent cell-mediated cytotoxicity in the Moloney sarcoma virus system: no requirement for exogenous C5. Clin Immunol Immunopathol. 1979 Sep;14(1):35–46. doi: 10.1016/0090-1229(79)90123-5. [DOI] [PubMed] [Google Scholar]
  7. Fuson E. W., Whitten H. D., Ayers R. D., Lamon E. W. Antibody-dependent cell-mediated cytotoxicity by human lymphocytes. I. Comparison of IgM- and IgG-induced cytotoxicity. J Immunol. 1978 May;120(5):1726–1732. [PubMed] [Google Scholar]
  8. Gately M. K., Martz E. Comparative studies on the mechanisms of nonspecific, Con A-dependent cytolysis and specific T cell-mediated cytolysis. J Immunol. 1977 Nov;119(5):1711–1722. [PubMed] [Google Scholar]
  9. Golstein P., Smith E. T. The lethal hit stage of mouse T and non-T cell-mediated cytolysis: differences in cation requirements and characterization of an analytical "cation pulse" method. Eur J Immunol. 1976 Jan;6(1):31–37. doi: 10.1002/eji.1830060108. [DOI] [PubMed] [Google Scholar]
  10. Griffin F. M., Jr, Bianco C., Silverstein S. C. Characterization of the macrophage receptro for complement and demonstration of its functional independence from the receptor for the Fc portion of immunoglobulin G. J Exp Med. 1975 Jun 1;141(6):1269–1277. doi: 10.1084/jem.141.6.1269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lamon E. W., Andersson B., Whitten H. D., Hurst M. M., Ghanta V. IgM complex receptors on subpopulations of murine lymphocytes. J Immunol. 1976 May;116(5):1199–1203. [PubMed] [Google Scholar]
  12. Lamon E. W., Shaw M. W., Goodson S., Lidin B., Walia A. S., Fuson E. W. Antibody-dependent cell-mediated cytotoxicity in the Moloney sarcoma virus system: differential activity of IgG and IgM with different subpopulations of lymphocytes. J Exp Med. 1977 Feb 1;145(2):302–313. doi: 10.1084/jem.145.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lamon E. W., Skurzak H. M., Andersson B., Whitten H. D., Klein E. Antibody-dependent lymphocyte cytotoxicity in the murine sarcoma virus system: activity of IgM and IgG with specificity for MLV determined antigen(s). J Immunol. 1975 Apr;114(4):1171–1176. [PubMed] [Google Scholar]
  14. Lamon E. W., Whitten H. D., Lidin B., Fudenberg H. H. IgM-induced tumor cell cytotoxicity mediated by normal thymocytes. J Exp Med. 1975 Aug 1;142(2):542–547. doi: 10.1084/jem.142.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lamon E. W., Whitten H. D., Skurzak H. M., Andersson B., Lidin B. IgM antibody-dependent cell-mediated cytotoxicity in the Moloney sarcoma virus system: the involvement of T and B lymphocytes as effector cells. J Immunol. 1975 Nov;115(5):1288–1294. [PubMed] [Google Scholar]
  16. Lay W. H., Nussenzweig V. Ca++-dependent binding of antigen-19 S antibody complexes to macrophages. J Immunol. 1969 May;102(5):1172–1178. [PubMed] [Google Scholar]
  17. Lay W. H., Nussenzweig V. Receptors for complement of leukocytes. J Exp Med. 1968 Nov 1;128(5):991–1009. doi: 10.1084/jem.128.5.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LoBuglio A. F., Cotran R. S., Jandl J. H. Red cells coated with immunoglobulin G: binding and sphering by mononuclear cells in man. Science. 1967 Dec 22;158(3808):1582–1585. doi: 10.1126/science.158.3808.1582. [DOI] [PubMed] [Google Scholar]
  19. Moretta L., Ferrarini M., Durante M. L., Mingari M. C. Expression of a receptor for IgM by human T cells in vitro. Eur J Immunol. 1975 Aug;5(8):565–569. doi: 10.1002/eji.1830050812. [DOI] [PubMed] [Google Scholar]
  20. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Plaut M., Bubbers J. E., Henney C. S. Studies of the mechanism of lymphocyte-mediated cytolysis. VII. Two stages in the T cell-mediated lytic cycle with distinct cation requirements. J Immunol. 1976 Jan;116(1):150–155. [PubMed] [Google Scholar]
  22. Spitler L., Huber H., Fudenberg H. H. Inhibition of capillary migration by antigen-antibody complexes. J Immunol. 1969 Feb;102(2):404–411. [PubMed] [Google Scholar]
  23. Walia A. S., Andersson B., Fuson E. W., Lamon E. W. The enrichment of thymocytes bearing C3 receptors following cortisone involution. Cell Immunol. 1979 Mar 1;43(1):176–184. doi: 10.1016/0008-8749(79)90160-6. [DOI] [PubMed] [Google Scholar]
  24. Webb S. R., Cooper M. D. T cells can bind antigen via cytophilic IgM antibody made by B cells. J Immunol. 1973 Jul;111(1):275–277. [PubMed] [Google Scholar]
  25. Wåhlin B., Perlmann H., Perlmann P. Analysis by a plaque assay of IgG- or IgM- dependent cytolytic lymphocytes in human blood. J Exp Med. 1976 Nov 2;144(5):1375–1380. doi: 10.1084/jem.144.5.1375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yoshida T. O., Andersson B. Evidence for a receptor recognizing antigen complexed immunoglobulin on the surface of activated mouse thymus lymphocytes. Scand J Immunol. 1972;1(4):401–408. doi: 10.1111/j.1365-3083.1972.tb03306.x. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES