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. 1980 Jul 1;152(1):113–123. doi: 10.1084/jem.152.1.113

Regulation of the immune response. I. The potentiation of in vivo and in vitro immune responses by Fc fragments

EL Morgan, SM Walker, ML Thoman, WO Weigle
PMCID: PMC2185893  PMID: 7400754

Abstract

Fc fragments derived from human and murine Ig were found to be potent adjuvants when administered with antigen. Both the in vivo and in vitro anti- sheep erythrocytes (SRBC) responses were significantly enhanced by Fc fragments. The adjuvant effect was shown to be extremely dependent upon the dose of antigen used, with the greatest enhancement occurring when suboptimal doses of antigen are employed. The anti-genicity of the Fc molecule was not related to its adjuvanticity because homologous Fc was as potent an adjuvant as heterologous Fc. Moreover, human Fc fragments enhanced anti-SRBC responses in mice which were tolerant to human gamma globulin.

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Selected References

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  1. Armerding D., Katz D. H. Activation of T and B lymphocytes in vitro. I. Regulatory influence of bacterial lipopolysaccharide (LPS) on specific T-cell helper function. J Exp Med. 1974 Jan 1;139(1):24–43. doi: 10.1084/jem.139.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berman M. A., Weigle W. O. B-lymphocytes activation by the Fc region of IgG. J Exp Med. 1977 Jul 1;146(1):241–256. doi: 10.1084/jem.146.1.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bloch-Shtacher N., Hirschhorn K., Uhr J. W. The response of lymphocytes from non-immunized humans to antigen-antibody complexes. Clin Exp Immunol. 1968 Nov;3(9):889–899. [PMC free article] [PubMed] [Google Scholar]
  4. Cerottini J. C., McConahey P. J., Dixon F. J. Specificity of the immunosuppression caused by passive administration of antibody. J Immunol. 1969 Aug;103(2):268–275. [PubMed] [Google Scholar]
  5. Cerottini J. C., McConahey P. J., Dixon F. J. The immunosuppressive effect of passively administered antibody IgG fragments. J Immunol. 1969 Apr;102(4):1008–1015. [PubMed] [Google Scholar]
  6. Dennert G. The mechanism of antibody-induced stimulation and inhibition of the immune response. J Immunol. 1971 Apr;106(4):951–955. [PubMed] [Google Scholar]
  7. Doyle M. V., Parks E., Weigle W. O. Specific, transient suppression of the immune response by HGG tolerant spleen cells. II. Effector cells and target cells. J Immunol. 1976 Oct;117(4):1152–1158. [PubMed] [Google Scholar]
  8. Golub E. S., Mishell R. I., Weigle W. O., Dutton R. W. A modification of the hemolytic plaque assay for use with protein antigens. J Immunol. 1968 Jan;100(1):133–137. [PubMed] [Google Scholar]
  9. JERNE N. K., NORDIN A. A. Plaque formation in agar by single antibody-producing cells. Science. 1963 Apr 26;140(3565):405–405. [PubMed] [Google Scholar]
  10. Kappler J. W., Hoffmann M., Dutton R. W. Regulation of the immune response. I. Differential effect of passively administered antibody on the thymus-derived and bone marrow-derived lymphocytes. J Exp Med. 1971 Sep 1;134(3 Pt 1):577–587. doi: 10.1084/jem.134.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lees R. K., Sinclair N. R. Regulation of the immune response. VII. In vitro immunosuppression by F(ab') 2 or intact IgG antibodies. Immunology. 1973 Apr;24(4):735–750. [PMC free article] [PubMed] [Google Scholar]
  12. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morgan E. L., Tempelis C. H. The requirement for the Fc portion of antibody in antigen-antibody complex-mediated suppression. J Immunol. 1978 May;120(5):1669–1671. [PubMed] [Google Scholar]
  14. Morgan E. L., Tempelis C. H. The role of antigen-antibody complexes in mediating immunologic unresponsiveness in the chicken. J Immunol. 1977 Oct;119(4):1293–1298. [PubMed] [Google Scholar]
  15. Morgan E. L., Weigle W. O. Polyclonal activation of murine B lymphocytes by Fc fragments. I. The requirement for two signals in the generation of the polyclonal antibody response induced by Fc fragments. J Immunol. 1980 Mar;124(3):1330–1335. [PubMed] [Google Scholar]
  16. Morgan E. L., Weigle W. O. Regulation of Fc fragment-induced murine spleen cell proliferation. J Exp Med. 1980 Jan 1;151(1):1–11. doi: 10.1084/jem.151.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morgan E. L., Weigle W. O. The requirement for adherent cells in the Fc fragment-induced proliferative response of murine spleen cells. J Exp Med. 1979 Aug 1;150(2):256–266. doi: 10.1084/jem.150.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morrison S. L., Terres G. Enhanced immunologic sensitization of mice by the simultaneous injection of antigen and specific antiserum. II. Effect of varying the antigen-antibody ratio and the amount of immune complex injected. J Immunol. 1966 May;96(5):901–905. [PubMed] [Google Scholar]
  19. Möller G., Coutinho A. Role of C'3 and Fc receptors in B-lymphocyte activation. J Exp Med. 1975 Mar 1;141(3):647–663. doi: 10.1084/jem.141.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nicholas R., Sinclair S. C. Regulation of the immune response. I. Reduction in ability of specific antibody to inhibit long-lasting IgG immunological priming after removal of the Fc fragment. J Exp Med. 1969 Jun 1;129(6):1183–1201. doi: 10.1084/jem.129.6.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ramasamy R. Role of membrane structures in B-lymphocyte activation. The effect of binding anti-immunoglobulin, aggregated IgG and immune complexes. Immunology. 1976 Apr;30(4):559–564. [PMC free article] [PubMed] [Google Scholar]
  23. Rowley D. A., Fitch F. W., Axelrad M. A., Pierce C. W. The immune response suppressed by specific antibody. Immunology. 1969 Apr;16(4):549–559. [PMC free article] [PubMed] [Google Scholar]
  24. Ryder R. J., Schwartz R. S. Immunosuppression by antibody: localization of site of action. J Immunol. 1969 Nov;103(5):970–978. [PubMed] [Google Scholar]
  25. STONER R. D., TERRES G. ENHANCED ANTITOXIN RESPONSES IN IRRADIATED MICE ELICITED BY COMPLEXES OF TETANUS TOXOID AND SPECIFIC ANTIBODY. J Immunol. 1963 Dec;91:761–770. [PubMed] [Google Scholar]
  26. Sjöberg O., Andersson J., Möller G. Lipopolysaccharide can substitute for helper cells in the antibody response in vitro. Eur J Immunol. 1972 Aug;2(4):326–331. doi: 10.1002/eji.1830020406. [DOI] [PubMed] [Google Scholar]
  27. Specter S., Cimprich R., Friedman H., Chedid L. Stimulation of an enhanced in vitro immune response by a synthetic adjuvant, muramyl dipeptide. J Immunol. 1978 Feb;120(2):487–491. [PubMed] [Google Scholar]
  28. Terres G., Habicht G. S., Stoner R. D. Carrier-specific enhancement of the immune response using antigen-antibody complexes. J Immunol. 1974 Feb;112(2):804–811. [PubMed] [Google Scholar]
  29. Terres G., Morrison S. L., Habicht G. S., Stoner R. D. Appearance of an early "primed state" in mice following the concomitant injections of antigen and specific antiserum. J Immunol. 1972 Jun;108(6):1473–1481. [PubMed] [Google Scholar]
  30. Walker J. G., Siskind G. W. Studies on the control of antibody synthesis. Effect of antibody affinity upon its ability to suppress antibody formation. Immunology. 1968 Jan;14(1):21–28. [PMC free article] [PubMed] [Google Scholar]

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