Abstract
We developed an idiotypic radioimmunoassay system that detects shared idiotypic determinants, termed GTGL idiotype, on antibodies bearing distinct antigen-binding specificities in various mouse strains. Either poly-(Glu, Tyr) (GT)- or poly-(Glu, Lys) (GL)-related determinants are able to induce anti-GT and anti-GL (GTGL)-idiotypic antibodies. Strain distribution studies indicate that GTGL-idiotypic antibodies are readily induced and frequently expressed in antisera obtained from 25 different mouse strains immunized either with GT-related or GL-related polymers. The ability to express GTGL-idiotypic antibodies is a dominant trait and is controlled by Igh-linked gene(s). In addition, we demonstrated that in anticopolymer of L-glutamine acid60- L-alanine30-L- tyrosine (GAT) and anticopolymer of L-glutamic acid54-L-lysine35-L- phenylalanine11(GLphi) antisera, both antibodies uniquely specific to GAT or GLphi, respectively, and antibodies bearing dual specificities for GAT and GLphi, expressed GTGL idiotype. The genetic implications of these findings are discussed. X
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Selected References
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- Bona C., Mond J. J., Stein K. E., House S., Lieberman R., Paul W. E. Immune response to levan. III. The capacity to produce anti-inulin antibodies and cross-reactive idiotypes appears late in ontogeny. J Immunol. 1979 Oct;123(4):1484–1490. [PubMed] [Google Scholar]
- Cheung N. K., Dorf M. E., Benacerraf B. Development of a hemolytic plaque assay for glutamic acid, lysine-containing polypeptides: demonstration that nonresponder mice produce antibodies to these peptides when conjugated to an immunogenic carrier. J Immunol. 1977 Sep;119(3):901–905. [PubMed] [Google Scholar]
- Eichmann K., Coutinho A., Melchers F. Absolute frequencies of lipopolysaccharide-reactive B cells producing A5A idiotype in unprimed, streptococcal A carbohydrate-primed, anti-A5A idiotype-sensitized and anti-A5A idiotype-suppressed A/J mice. J Exp Med. 1977 Nov 1;146(5):1436–1449. doi: 10.1084/jem.146.5.1436. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ju S. T., Benacerraf B., Dorf M. E. Idiotypic analysis of antibodies to poly(Glu60Ala30Tyr10): interstrain and interspecies idiotypic crossreactions. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6192–6196. doi: 10.1073/pnas.75.12.6192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ju S. T., Dorf M. E., Benacerraf B. Idiotypic analysis of anti-GAT antibodies. III. Determinant specificity and immunoglobulin class distribution of CGAT idiotype. J Immunol. 1979 Mar;122(3):1054–1058. [PubMed] [Google Scholar]
- Ju S. T., Kipps T. J., Theze J., Benacerraf B., Dorf M. E. Idiotypic analysis of anti-GAT antibodies. I. Presence of common idiotypic specificities in both responder and nonresponder mice. J Immunol. 1978 Sep;121(3):1034–1039. [PubMed] [Google Scholar]
- Ju S. T., Pierres M., Waltenbaugh C., Germain R. N., Benacerraf B., Dorf M. E. Idiotypic analysis of monoclonal antibodies to poly(Glu60Ala30Tyr10). Proc Natl Acad Sci U S A. 1979 Jun;76(6):2942–2946. doi: 10.1073/pnas.76.6.2942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kabat E. A., Wu T. T., Bilofsky H. Variable region genes for the immunoglobulin framework are assembled from small segments of DNA--a hypothesis. Proc Natl Acad Sci U S A. 1978 May;75(5):2429–2433. doi: 10.1073/pnas.75.5.2429. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karol R., Reichlin M., Noble R. W. Idiotypic cross-reactivity between antibodies of different specificities. J Exp Med. 1978 Dec 1;148(6):1488–1497. doi: 10.1084/jem.148.6.1488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oudin J., Cazenave P. A. Similar idiotypic specificities in immunoglobulin fractions with different antibody functions or even without detectable antibody function. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2616–2620. doi: 10.1073/pnas.68.10.2616. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierres M., Ju S. T., Waltenbaugh C., Dorf M. E., Benacerraf B., Germain R. N. Fine specificity of antibodies to poly(Glu60Ala30Tyr10) produced by hybrid cell lines. Proc Natl Acad Sci U S A. 1979 May;76(5):2425–2429. doi: 10.1073/pnas.76.5.2425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SLATER R. J., WARD S. M., KUNKEL H. G. Immunological relationships among the myeloma proteins. J Exp Med. 1955 Jan 1;101(1):85–108. doi: 10.1084/jem.101.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]