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. 1978 Nov;136(2):688–692. doi: 10.1128/jb.136.2.688-692.1978

Classes of Anabaena variabilis mutants with oxygen-sensitive nitrogenase activity.

J F Haury, C P Wolk
PMCID: PMC218594  PMID: 101529

Abstract

Mutants of Anabaena variabilis deficient in the envelope glycolipids of heterocysts have no or very low nitrogenase activity when assayed aerobically. Revertants capable of aerobic growth on N2 have increased quantities of these glycolipids. Among mutants which require fixed nitrogen for growth in air and which have a normal complement of glycolipids, one expresses high nitrogenase activity at low oxygen tension. Three others show high nitrogenase activity only in the presence of dithionite and are therefore impaired in electron transfer.

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Selected References

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  1. Allen M. B., Arnon D. I. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm. Plant Physiol. 1955 Jul;30(4):366–372. doi: 10.1104/pp.30.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Currier T. C., Haury J. F., Wolk C. P. Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium. J Bacteriol. 1977 Mar;129(3):1556–1562. doi: 10.1128/jb.129.3.1556-1562.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fay P., Stewart W. D., Walsby A. E., Fogg G. E. Is the heterocyst the site of nitrogen fixation in blue-green algae? Nature. 1968 Nov 23;220(5169):810–812. doi: 10.1038/220810b0. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Lambein F., Wolk C. P. Structural studies on the glycolipids from the envelope of the heterocyst of Anabaena cylindrica. Biochemistry. 1973 Feb 27;12(5):791–798. doi: 10.1021/bi00729a002. [DOI] [PubMed] [Google Scholar]
  6. Lockau W., Peterson R. B., Wolk C. P., Burris R. H. Modes of reduction of nitrogen in heterocysts isolated from Anabaena species. Biochim Biophys Acta. 1978 May 10;502(2):298–308. doi: 10.1016/0005-2728(78)90051-8. [DOI] [PubMed] [Google Scholar]
  7. Peterson R. B., Burris R. H. Properties of heterocysts isolated with colloidal silica. Arch Microbiol. 1976 May 3;108(1):35–40. doi: 10.1007/BF00425090. [DOI] [PubMed] [Google Scholar]
  8. Peterson R. B., Wolk C. P. Localization of an uptake hydrogenase in anabaena. Plant Physiol. 1978 Apr;61(4):688–691. doi: 10.1104/pp.61.4.688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. St John R. T., Johnston H. M., Seidman C., Garfinkel D., Gordon J. K., Shah V. K., Brill W. J. Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2. J Bacteriol. 1975 Mar;121(3):759–765. doi: 10.1128/jb.121.3.759-765.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stewart W. D., Haystead A., Pearson H. W. Nitrogenase activity in heterocysts of blue-green algae. Nature. 1969 Oct 18;224(5216):226–228. doi: 10.1038/224226a0. [DOI] [PubMed] [Google Scholar]
  11. Stewart W. D. Nitrogen fixation by photosynthetic microorganisms. Annu Rev Microbiol. 1973;27:283–316. doi: 10.1146/annurev.mi.27.100173.001435. [DOI] [PubMed] [Google Scholar]
  12. Stewart W. D., Rowell P. Effects of L-methionine-DL-sulphoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production in Anabaena cylindrica. Biochem Biophys Res Commun. 1975 Aug 4;65(3):846–856. doi: 10.1016/s0006-291x(75)80463-3. [DOI] [PubMed] [Google Scholar]
  13. Thomas J., Meeks J. C., Wolk C. P., Shaffer P. W., Austin S. M. Formation of glutamine from [13n]ammonia, [13n]dinitrogen, and [14C]glutamate by heterocysts isolated from Anabaena cylindrica. J Bacteriol. 1977 Mar;129(3):1545–1555. doi: 10.1128/jb.129.3.1545-1555.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wolk C. P., Austin S. M., Bortins J., Galonsky A. Autoradiographic localization of 13N after fixation of 13N-labeled nitrogen gas by a heterocyst-forming blue-green alga. J Cell Biol. 1974 May;61(2):440–453. doi: 10.1083/jcb.61.2.440. [DOI] [PMC free article] [PubMed] [Google Scholar]

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