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. 1980 Aug 1;152(2):377–390. doi: 10.1084/jem.152.2.377

Cytopathogenic mechanisms of Entamoeba histolytica

PMCID: PMC2185944  PMID: 6249882

Abstract

Cinemicrography of Entamoeba histolytica destruction of Chinese hamster ovary (CHO) cells shows that ameba cytopathogenicity consists of separate components: a contact-dependent cytolethal effect, and phagocytosis. Cells not in contact with amebae remain intact. Quantitation of ameba destruction of CHO cells by applying the one-hit hypothesis confirms that the cytoethal effect of amebae is contact dependent. Studies with 111Indium oxine-labeled cells provide further evidence of extracellular killing by E. histolytica and indicate that > 94% of the target cells are killed before phagocytosis. When we examined for a cytotoxin release by E. histolytica, we found no effect on CHO cells with filtrates of amebae, and a nonspecific effect of cell rounding and release with sonicates of amebae. The ameba sonicate effect was time-dose dependent, was not cytolethal, was reversible, and was inhibited by alpha II macroglobulin. Cytochalasin B altered ameba motility and morphology, and monolayer experiments confirmed that cytochalasins A, B, or D inhibited CHO cell destruction by E. histolytica. Cytochalasin D also inhibited extracellular killing of CHO cells by amebae in pellets, apparently independent of effects on ameba motility or phagocytosis. Colchicine and vinblastine, alone or in combination with cytochalasin D, did not inhibit E. histolytica cytopathogenicity, which indicates that microtubule function is not required for target cell killing by amebae.

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Selected References

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  1. Allison A. C., Ferluga J. Editorial: How lymphocytes kill tumor cells. N Engl J Med. 1976 Jul 15;295(3):165–167. doi: 10.1056/NEJM197607152950311. [DOI] [PubMed] [Google Scholar]
  2. Asherson G. L., Ferluga J. Contact sensitivity in the mouse. X. Non-specific cytotoxicity of T blasts in the draining lymph nodes. Immunology. 1973 Sep;25(3):471–483. [PMC free article] [PubMed] [Google Scholar]
  3. Asherson G. L., Ferluga J., Janossy G. Non-specific cytotoxicity by T cells activated with plant mitogens in vitro and the requirement for plant agents during the killing reaction. Clin Exp Immunol. 1973 Dec;15(4):573–589. [PMC free article] [PubMed] [Google Scholar]
  4. Bryan J. Definition of three classes of binding sites in isolated microtubule crystals. Biochemistry. 1972 Jul 4;11(14):2611–2616. doi: 10.1021/bi00764a010. [DOI] [PubMed] [Google Scholar]
  5. Czech M. P., Lynn D. G., Lynn W. S. Cytochalasin B-sensitive 2-deoxy-D-glucose transport in adipose cell ghosts. J Biol Chem. 1973 May 25;248(10):3636–3641. [PubMed] [Google Scholar]
  6. Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
  7. Diamond L. S. Techniques of axenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae. J Parasitol. 1968 Oct;54(5):1047–1056. [PubMed] [Google Scholar]
  8. Eaton R. D., Meerovitch E., Costerton J. W. A surface-active lysosome in Entamoeba histolytica. Trans R Soc Trop Med Hyg. 1969;63(5):678–680. doi: 10.1016/0035-9203(69)90191-6. [DOI] [PubMed] [Google Scholar]
  9. Frost P., Smith J., Frost H. The radiolabeling of lymphocytes and tumor cells with 111indium. Proc Soc Exp Biol Med. 1978 Jan;157(1):61–65. doi: 10.3181/00379727-157-39991. [DOI] [PubMed] [Google Scholar]
  10. Griffin J. L. Human amebic dysentery. Electron microscopy of Entamoeba histolytica contacting, ingesting, and digesting inflammatory cells. Am J Trop Med Hyg. 1972 Nov;21(6):895–906. [PubMed] [Google Scholar]
  11. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Henney C. S. T cell mediated cytolysis: consideration of the role of a soluble mediator. J Reticuloendothel Soc. 1975 Apr;17(4):231–235. [PubMed] [Google Scholar]
  13. Henson P. M., Oades Z. G. Enhancement of immunologically induced granule exocytosis from neutrophils by cytochalasin B. J Immunol. 1973 Jan;110(1):290–293. [PubMed] [Google Scholar]
  14. JARUMILINTA R., KRADOLFER F. THE TOXIC EFFECT OF ENTAMOEBA HISTOLYTICA ON LEUCOCYTES. Ann Trop Med Parasitol. 1964 Sep;58:375–381. doi: 10.1080/00034983.1964.11686259. [DOI] [PubMed] [Google Scholar]
  15. Knight R. An in vitro model for measuring the cytopathic effect of Entamoeba histolytica. J Parasitol. 1977 Apr;63(2):388–389. [PubMed] [Google Scholar]
  16. Knight R., Bird R. G., McCaul T. F. Fine structural changes at Entamoeba histolytica rabbit kidney cell (RK 13) interface. Ann Trop Med Parasitol. 1975 Jun;69(2):197–202. doi: 10.1080/00034983.1975.11687001. [DOI] [PubMed] [Google Scholar]
  17. Lushbaugh W. B., Kairalla A. B., Cantey J. R., Hofbauer A. F., Pittman F. E. Isolation of a cytotoxin-enterotoxin from Entamoeba histolytica. J Infect Dis. 1979 Jan;139(1):9–17. doi: 10.1093/infdis/139.1.9. [DOI] [PubMed] [Google Scholar]
  18. Mattern C. F., Keister D. B., Caspar P. A. Experimental amebiasis. III. A rapid in vitro assay for virulence of Entamoeba histolytica. Am J Trop Med Hyg. 1978 Sep;27(5):882–887. [PubMed] [Google Scholar]
  19. McCaul T. F., Bird R. G. Surface features of Entamoeba histolytica and rabbit kidney (RK13) cell surface changes after trophozoite contact--observations by scanning electron microscopy. Int J Parasitol. 1977 Oct;7(5):383–388. doi: 10.1016/0020-7519(77)90063-7. [DOI] [PubMed] [Google Scholar]
  20. McCaul T. F., Poston R. N., Bird R. G. Entamoeba histolytica and Entamoeba invadens: chromium release from labeled human liver cells in culture. Exp Parasitol. 1977 Dec;43(2):342–352. doi: 10.1016/0014-4894(77)90039-x. [DOI] [PubMed] [Google Scholar]
  21. Pesanti E. L., Axline S. G. Phagolysosome formation in normal and colchicine-treated macrophages. J Exp Med. 1975 Oct 1;142(4):903–913. doi: 10.1084/jem.142.4.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Phillips B. P., Diamond L. S., Bartgis I. L., Stuppler S. A. Results of intracecal inoculation of germfree and conventional guinea pigs and germfree rats with axenically cultivated Entamoeba histolytica. J Protozool. 1972 Aug;19(3):498–499. doi: 10.1111/j.1550-7408.1972.tb03512.x. [DOI] [PubMed] [Google Scholar]
  23. Proctor E. M., Gregory M. A. The observation of a surface active lysosome in the trophozoites of Entamoeba histolytica from the human colon. Ann Trop Med Parasitol. 1972 Sep;66(3):339–342. doi: 10.1080/00034983.1972.11686833. [DOI] [PubMed] [Google Scholar]
  24. Rondanelli E. G., Carosi G., Filice G., De Carneri I. Observation of surface active lysosomes and other organelles in monoxenically cultivated trophozoites of ent amoeba histolytica and Ent amoeba coli. Int J Parasitol. 1974 Aug;4(4):433–435. doi: 10.1016/0020-7519(74)90053-8. [DOI] [PubMed] [Google Scholar]
  25. Rosenbaum R. M., Wittner M. Ultrastructure of bacterized and axenic trophozoites of Entamoeba histolytica with particular reference to helical bodies. J Cell Biol. 1970 May;45(2):367–382. doi: 10.1083/jcb.45.2.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sanderson C. J. The mechanism of T cell mediated cytotoxicity. I. The release of different cell components. Proc R Soc Lond B Biol Sci. 1976 Jan 20;192(1107):221–239. doi: 10.1098/rspb.1976.0010. [DOI] [PubMed] [Google Scholar]
  27. Sanderson C. J. The mechanism of T cell mediated cytotoxicity. II. Morphological studies of cell death by time-lapse microcinematography. Proc R Soc Lond B Biol Sci. 1976 Jan 20;192(1107):241–255. doi: 10.1098/rspb.1976.0011. [DOI] [PubMed] [Google Scholar]
  28. Sharma S. D., Piessens W. F. Tumor cell killing by macrophages activated in vitro with lymphocyte mediators. III. Inhibition by cytochalasins, colchicine, and vinblastine. Cell Immunol. 1978 Jul;38(2):276–285. doi: 10.1016/0008-8749(78)90058-8. [DOI] [PubMed] [Google Scholar]
  29. Wilson L. Action of drugs on microtubules. Life Sci. 1975 Aug 1;17(3):303–309. doi: 10.1016/0024-3205(75)90476-2. [DOI] [PubMed] [Google Scholar]
  30. Wiltrout R. H., Frost P., Cummings G. D. Isotope-release cytotoxicity assay with the use of indium-111: advantage over chromium-51 in long-term assays. J Natl Cancer Inst. 1978 Jul;61(1):183–188. doi: 10.1093/jnci/61.1.183. [DOI] [PubMed] [Google Scholar]
  31. Wittner M. Growth characteristics of axenic strains of Entamoeba histolytica Schaudinn, 1903. J Protozool. 1968 Aug;15(3):403–406. doi: 10.1111/j.1550-7408.1968.tb02147.x. [DOI] [PubMed] [Google Scholar]
  32. el-Hashimi W., Pittman F. Ultrastructure of Entamoeba histolytica trophozoites obtained from the colon and from in vitro cultures. Am J Trop Med Hyg. 1970 Mar;19(2):215–226. doi: 10.4269/ajtmh.1970.19.215. [DOI] [PubMed] [Google Scholar]

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