Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1980 Oct 1;152(4):893–904. doi: 10.1084/jem.152.4.893

Concanavalin A-inducible, interleukin-2-producing T cell hybridoma

PMCID: PMC2185978  PMID: 6968339

Abstract

The fusion of an AKR T cell tumor line to normal B6D2F1, T cells resulted in the production of a cloned T cell hybridoma (FS6-14.13) inducible with the mitogen concanavalin A (Con A). The supernate from Con A-stimulated hybridoma cells was active both in the stimulation of an anti-sheep red blood cell response by partially T cell-depleted B cells and in the stimulation of the growth of antigen-specific T cell blasts. The active principle in both assays had a molecular weight of approximately 30-40,000. These results indicated the presence of interleukin 2 (IL2) in the hybridoma supernate. The activity of the hybridoma supernate in B cell responses was dependent on the presence of adherent cells and a few contaminating T cells. On the other hand, Con A-stimulated supernates from normal spleen cells were active after either adherent cell removal or severe T cell depletion. These results suggested that IL2 was the only active helper factor in the hybridoma supernate, but that additional helper factors were present in supernates from Con A-stimulated normal spleen cells.

Full Text

The Full Text of this article is available as a PDF (691.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergman Y., Haimovich J. Characterization of a carcinogen-induced murine B lymphocyte cell line of C3H/eB origin. Eur J Immunol. 1977 Jul;7(7):413–417. doi: 10.1002/eji.1830070702. [DOI] [PubMed] [Google Scholar]
  2. Gillis S., Smith K. A., Watson J. Biochemical characterization of lymphocyte regulatory molecules. II. Purification of a class of rat and human lymphokines. J Immunol. 1980 Apr;124(4):1954–1962. [PubMed] [Google Scholar]
  3. Harwell L., Kappler J. W., Marrack P. Antigen-specific and nonspecific mediators of T cell/B cell cooperation. III. Characterization of the nonspecific mediator(s) from different sources. J Immunol. 1976 May;116(5):1379–1384. [PubMed] [Google Scholar]
  4. Hoffmann M. K., Watson J. Helper T cell-replacing factors secreted by thymus-derived cells and macrophages: cellular requirements for B cell activation and synergistic properties. J Immunol. 1979 Apr;122(4):1371–1375. [PubMed] [Google Scholar]
  5. JERNE N. K., NORDIN A. A. Plaque formation in agar by single antibody-producing cells. Science. 1963 Apr 26;140(3565):405–405. [PubMed] [Google Scholar]
  6. Kappler J. W. A micro-technique for hemolytic plaque assays. J Immunol. 1974 Mar;112(3):1271–1274. [PubMed] [Google Scholar]
  7. Kappler J. W., Marrack P. The role of H-2-linked genes in helper T-cell function. I. In vitro expression in B cells of immune response genes controlling helper T-cell activity. J Exp Med. 1977 Dec 1;146(6):1748–1764. doi: 10.1084/jem.146.6.1748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Katz D. H., Osborne D. P., Jr The allogeneic effect in inbred mice. II. Establishment of the cellular interactions required for enhancement of antibody production by the graft-versus-host reaction. J Exp Med. 1972 Sep 1;136(3):455–465. doi: 10.1084/jem.136.3.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keller D. M., Swierkosz J. E., Marrack P., Kappler J. W. Two types of functionally distinct, synergizing helper T cells. J Immunol. 1980 Mar;124(3):1350–1359. [PubMed] [Google Scholar]
  10. Kontiainen S., Simpson E., Bohrer E., Beverley P. C., Herzenberg L. A., Fitzpatrick W. C., Vogt P., Torano A., McKenzie I. F., Feldmann M. T-cell lines producing antigen-specific suppressor factor. Nature. 1978 Aug 3;274(5670):477–480. doi: 10.1038/274477a0. [DOI] [PubMed] [Google Scholar]
  11. Marrack P., Kappler J. W. Antigen-specific and nonspecific mediatiors of T cell/B cell cooperation. II. Two helper T cells distinguished by their antigen sensitivities. J Immunol. 1976 May;116(5):1373–1378. [PubMed] [Google Scholar]
  12. McDougal J. S., Gordon D. S. Generation of T-helper cells in vitro. II. Analysis of supernates derived from T-helper cell cultures. J Exp Med. 1977 Mar 1;145(3):693–708. doi: 10.1084/jem.145.3.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schrier R. D., Skidmore B. J., Kurnick J. T., Goldstine S. N., Chiller J. M. Propagation of antigen-specific T cell helper function in vitro. J Immunol. 1979 Dec;123(6):2525–2531. [PubMed] [Google Scholar]
  15. Skidmore B. J., Miller L. S. The determination of lymphoid cell chimerism using peripheral blood lymphocytes from murine bone marrow chimeras. J Immunol Methods. 1978;24(3-4):337–343. doi: 10.1016/0022-1759(78)90136-9. [DOI] [PubMed] [Google Scholar]
  16. Smith K. A., Gillis S., Baker P. E., McKenzie D., Ruscetti F. W. T-cell growth factor-mediated T-cell proliferation. Ann N Y Acad Sci. 1979;332:423–432. doi: 10.1111/j.1749-6632.1979.tb47136.x. [DOI] [PubMed] [Google Scholar]
  17. Swain S. L., Dutton R. W. Production of Con A-induced helper T cell replacing factor requires a T cell and an Ia-positive non-T cells. J Immunol. 1980 Jan;124(1):437–444. [PubMed] [Google Scholar]
  18. Taussig M. J., Munro A. J., Campbell R., David C. S., Staines N. A. Antigen-specific T-cell factor in cell cooperation. Mapping within the I region of the H-2 complex and ability to cooperate across allogeneic barriers. J Exp Med. 1975 Sep 1;142(3):694–700. doi: 10.1084/jem.142.3.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Waldmann H., Munro A. T cell-dependent mediator in the immune response. II. Physical and biological properties. Immunology. 1974 Jul;27(1):53–64. [PMC free article] [PubMed] [Google Scholar]
  20. Watson J., Aarden L. A., Shaw J., Paetkau V. Molecular and quantitative analysis of helper T cell-replacing factors on the induction of antigen-sensitive B and T lymphocytes. J Immunol. 1979 May;122(5):1633–1638. [PubMed] [Google Scholar]
  21. Watson J., Gillis S., Marbrook J., Mochizuki D., Smith K. A. Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines. J Exp Med. 1979 Oct 1;150(4):849–861. doi: 10.1084/jem.150.4.849. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES