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. 1980 Nov 1;152(5):1135–1146. doi: 10.1084/jem.152.5.1135

Regulation of murine B cells through surface immunoglobulin. I. Monoclonal anti-delta antibody that induces allotype-specific proliferation

PMCID: PMC2185997  PMID: 6776225

Abstract

We describe the identification of a monoclonal antibody that recognizes a determinant on the delta chain of mice of the Iga, allotype groups. The monoclonal Ig in soluble form induces allotype-specific proliferation by splenic B lymphocytes from normal animals of these haplotypes. Spleen cells from mice bearing the X-linked defect of CBA/N mice fail to respond, although they bear the determinant. Proliferation is independent of T lymphocytes. The data indicate a direct triggering function for sIgD.

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Selected References

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  1. Ahmed A., Scher I., Sharrow S. O., Smith A. H., Paul W. E., Sachs D. H., Sell K. W. B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens. J Exp Med. 1977 Jan 1;145(1):101–110. doi: 10.1084/jem.145.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buck L. B., Yuan D., Vitetta E. S. A dichotomy between the expression of IgD on B cells and its requirement for triggering such cells with two T-independent antigens. J Exp Med. 1979 Apr 1;149(4):987–992. doi: 10.1084/jem.149.4.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cambier J. C., Ligler F. S., Uhr J. W., Kettman J. R., Vitetta E. S. Blocking of primary in vitro antibody responses to thymus-independent and thymus-dependent antigens with antiserum specific for IgM or IgD. Proc Natl Acad Sci U S A. 1978 Jan;75(1):432–435. doi: 10.1073/pnas.75.1.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coffino P., Baumal R., Laskov R., Scharff M. D. Cloning of mouse myeloma cells and detection of rare variants. J Cell Physiol. 1972 Jun;79(3):429–440. doi: 10.1002/jcp.1040790313. [DOI] [PubMed] [Google Scholar]
  5. Cullen S. E., Schwartz B. D., Nathenson S. G. The distribution of alloantigenic specificities of native H-2 products. J Immunol. 1972 Mar;108(3):596–600. [PubMed] [Google Scholar]
  6. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  7. Finkelman F. D., Kessler S. W., Scher I. Preparation of an antibody to mouse serum IgD. J Immunol. 1979 Aug;123(2):952–954. [PubMed] [Google Scholar]
  8. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  9. Goding J. W. Allotypes of IgM and IgD receptors in the mouse: a probe for lymphocyte differentiation. Contemp Top Immunobiol. 1978;8:203–243. doi: 10.1007/978-1-4684-0922-2_7. [DOI] [PubMed] [Google Scholar]
  10. Huber B., Gershon R. K., Cantor H. Identification of a B-cell surface structure involved in antigen-dependent triggering: absence of this structure on B cells from CBA/N mutant mice. J Exp Med. 1977 Jan 1;145(1):10–20. doi: 10.1084/jem.145.1.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  12. Kessler S. W., Woods V. L., Finkelman F. D., Scher I. Membrane orientation and location of multiple and distinct allotypic determinants of mouse lymphocyte IgD. J Immunol. 1979 Dec;123(6):2772–2778. [PubMed] [Google Scholar]
  13. Kettman J. R., Cambier J. C., Uhr J. W., Ligler F., Vitetta E. S. The role of receptor IgM and IgD in determining triggering and induction of tolerance in murine B cells. Immunol Rev. 1979;43:69–95. doi: 10.1111/j.1600-065x.1979.tb00418.x. [DOI] [PubMed] [Google Scholar]
  14. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  17. Morgan E. L., Weigle W. O. The requirement for adherent cells in the Fc fragment-induced proliferative response of murine spleen cells. J Exp Med. 1979 Aug 1;150(2):256–266. doi: 10.1084/jem.150.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Möller G. One non-specific signal triggers b lymphocytes. Transplant Rev. 1975;23:126–137. [PubMed] [Google Scholar]
  19. Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
  20. Parker D. C. Stimulation of mouse lymphocytes by insoluble anti-mouse immunoglobulin. Nature. 1975 Nov 27;258(5533):361–363. doi: 10.1038/258361a0. [DOI] [PubMed] [Google Scholar]
  21. Sachs D. H., Winn H. J., Russell P. S. The immunologic response to xenografts. Recognition of mouse H-2 histocompatibility antigens by the rat. J Immunol. 1971 Aug;107(2):481–492. [PubMed] [Google Scholar]
  22. Shulman M., Wilde C. D., Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. doi: 10.1038/276269a0. [DOI] [PubMed] [Google Scholar]
  23. Sidman C. L., Unanue E. R. Control of proliferation and differentiation in B lymphocytes by anti-Ig antibodies and a serum-derived cofactor. Proc Natl Acad Sci U S A. 1978 May;75(5):2401–2405. doi: 10.1073/pnas.75.5.2401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sieckmann D. G., Asofsky R., Mosier D. E., Zitron I. M., Paul W. E. Activation of mouse lymphocytes by anti-immunoglobulin. I. Parameters of the proliferative response. J Exp Med. 1978 Mar 1;147(3):814–829. doi: 10.1084/jem.147.3.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Subbarao B., Mosier D. E., Ahmed A., Mond J. J., Scher I., Paul W. E. Role of a nonimmunoglobulin cell surface determinant in the activation of B lymphocytes by thymus-independent antigens. J Exp Med. 1979 Feb 1;149(2):495–506. doi: 10.1084/jem.149.2.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Vitetta E. S., Uhr J. W. Immunoglobulin-receptors revisited. Science. 1975 Sep 19;189(4207):964–969. doi: 10.1126/science.1083069. [DOI] [PubMed] [Google Scholar]
  27. Warner N. L. Membrane immunoglobulins and antigen receptors on B and T lymphocytes. Adv Immunol. 1974;19(0):67–216. doi: 10.1016/s0065-2776(08)60252-7. [DOI] [PubMed] [Google Scholar]
  28. Weiner H. L., Moorhead J. W., Claman H. N. Anti-immunoglobulin stimulation of murine lymphocytes. I. Age dependency of the proliferative response. J Immunol. 1976 Jun;116(6):1656–1661. [PubMed] [Google Scholar]
  29. Zitron I. M., Mosier D. E., Paul W. E. The role of surface IgD in the response to thymic-independent antigens. J Exp Med. 1977 Dec 1;146(6):1707–1718. doi: 10.1084/jem.146.6.1707. [DOI] [PMC free article] [PubMed] [Google Scholar]

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