Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1980 Dec 1;152(6):1497–1505. doi: 10.1084/jem.152.6.1497

Serological identification and cellular distribution of three F9 antigen components

PMCID: PMC2186040  PMID: 6161199

Abstract

Using an affinity chromatography technique, IgM, IgG1, IgG2a,b anti-F9 antibodies have been isolated from the anti-F9 serum; their activities have been analyzed by IF test on a variety of cell types, teratocarcinoma-derived cell lines, and embryos. The anti-F9 antibodies react with at least three independent antigenic determinants not expressed on the same cell types, and that appear along different time- course during embryonic development.

Full Text

The Full Text of this article is available as a PDF (551.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artzt K., Bennett D., Jacob F. Primitive teratocarcinoma cells express a differentiation antigen specified by a gene at the T-locus in the mouse. Proc Natl Acad Sci U S A. 1974 Mar;71(3):811–814. doi: 10.1073/pnas.71.3.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Artzt K., Dubois P., Bennett D., Condamine H., Babinet C., Jacob F. Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2988–2992. doi: 10.1073/pnas.70.10.2988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berstine E. G., Hooper M. L., Grandchamp S., Ephrussi B. Alkaline phosphatase activity in mouse teratoma. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899–3903. doi: 10.1073/pnas.70.12.3899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boccara M., Kelly F. Expression of polyoma virus in heterokaryons between embryonal carcinoma cells and differentiated cells. Virology. 1978 Oct 1;90(1):147–150. doi: 10.1016/0042-6822(78)90342-2. [DOI] [PubMed] [Google Scholar]
  5. Boon T., Buckingham M. E., Dexter D. L., Jakob H., Jacob F. Tératocarcinome de la souris: isolement et propriétés de deux lignées de myoblastes. Ann Microbiol (Paris) 1974 Jul-Aug;125B(1):13–28. [PubMed] [Google Scholar]
  6. Buc-Caron M. H., Condamine H., Jacob F. The presence of F9 antigen on the surface of mouse embryonic cells until day 8 of embryogenesis. J Embryol Exp Morphol. 1978 Oct;47:149–160. [PubMed] [Google Scholar]
  7. Buc-Caron M. H., Dupouey P. On the nature of F9 antigenic determinants. Mol Immunol. 1980 May;17(5):655–664. doi: 10.1016/0161-5890(80)90164-9. [DOI] [PubMed] [Google Scholar]
  8. Cheng C. C., Bennett D. Nature of the antigenic determinants of T locus antigens. Cell. 1980 Feb;19(2):537–543. doi: 10.1016/0092-8674(80)90528-0. [DOI] [PubMed] [Google Scholar]
  9. Damonneville M., Morello D., Gachelin G., Stanislawski M. Antibody response to embryonal carcinoma cells in syngeneic mice. Eur J Immunol. 1979 Dec;9(12):932–937. doi: 10.1002/eji.1830091205. [DOI] [PubMed] [Google Scholar]
  10. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  11. Gachelin G., Fellous M., Guenet J. L., Jacob F. Developmental expression of an early embryonic antigen common to mouse spermatozoa and cleavage embryos, and to human spermatozoa: its expression during spermatogenesis. Dev Biol. 1976 Jun;50(2):310–320. doi: 10.1016/0012-1606(76)90154-8. [DOI] [PubMed] [Google Scholar]
  12. Gachelin G. The cell surface antigens of mouse embryonal carcinoma cells. Biochim Biophys Acta. 1978 Sep 18;516(1):27–60. doi: 10.1016/0304-419x(78)90003-3. [DOI] [PubMed] [Google Scholar]
  13. Gooding L. R., Edidin M. Cell surface antigens of a mouse testicular teratoma. Identification of an antigen physically associated with H-2 antigens on tumor cells. J Exp Med. 1974 Jul 1;140(1):61–78. doi: 10.1084/jem.140.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gooding L. R., Hsu Y. C., Edidin M. Expression of teratoma-associated antigens on murine ova and early embryos. Identification of two early differentiation markers. Dev Biol. 1976 Apr;49(2):479–486. doi: 10.1016/0012-1606(76)90189-5. [DOI] [PubMed] [Google Scholar]
  15. Holden S., Bernard O., Artzt K., Whitmore W. F., Jr, Bennett D. Human and mouse embryonal carcinoma cells in culture share an embryonic antigen (F9). Nature. 1977 Dec 8;270(5637):518–520. doi: 10.1038/270518a0. [DOI] [PubMed] [Google Scholar]
  16. Jacob F. Mouse teratocarcinoma and embryonic antigens. Immunol Rev. 1977 Jan;33:3–32. doi: 10.1111/j.1600-065x.1977.tb00360.x. [DOI] [PubMed] [Google Scholar]
  17. Jakob H., Boon T., Gaillard J., Nicolas J., Jacob F. Tératocarcinome de la spuris: isolement, culture et propriétés de cellules a potentialités multiples. Ann Microbiol (Paris) 1973 Oct;124(3):269–282. [PubMed] [Google Scholar]
  18. Jakob H., Dubois P., Eisen H., Jacob F. Effets de l'hexaméthylènebisacétamide sur la différenciation de cellules de carcinome embryonnaire. C R Acad Sci Hebd Seances Acad Sci D. 1978 Jan;286(1):109–111. [PubMed] [Google Scholar]
  19. Kelly F., Sambrook J. Variants of simian virus 40-transformed mouse cells resistant to cytochalasin B. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):345–353. doi: 10.1101/sqb.1974.039.01.045. [DOI] [PubMed] [Google Scholar]
  20. Kemler R., Babinet C., Condamine H., Gachelin G., Guenet J. L., Jacob F. Embryonal carcinoma antigen and the T/t locus of the mouse. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4080–4084. doi: 10.1073/pnas.73.11.4080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lehman J. M., Speers W. C., Swartzendruber D. E., Pierce G. B. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J Cell Physiol. 1974 Aug;84(1):13–27. doi: 10.1002/jcp.1040840103. [DOI] [PubMed] [Google Scholar]
  22. Martin G. R., Evans M. J. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1441–1445. doi: 10.1073/pnas.72.4.1441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. McBurney M. W. Clonal lines of teratocarcinoma cells in vitro: differentiation and cytogenetic characteristics. J Cell Physiol. 1976 Nov;89(3):441–455. doi: 10.1002/jcp.1040890310. [DOI] [PubMed] [Google Scholar]
  24. Morello D., Gachelin G., Dubois P., Tanigaki N., Pressman D., Jacob F. Absence of reaction of a xenogenic anti-H-2 serum with mouse embryonal carcinoma cells. Transplantation. 1978 Aug;26(2):119–125. doi: 10.1097/00007890-197808000-00012. [DOI] [PubMed] [Google Scholar]
  25. Muramatsu T., Gachelin G., Damonneville M., Delarbre C., Jacob F. Cell surface carbohydrates of embryonal carcinoma cells: polysaccharidic side chains of F9 antigens and of receptors to two lectins, FBP and PNA. Cell. 1979 Sep;18(1):183–191. doi: 10.1016/0092-8674(79)90367-2. [DOI] [PubMed] [Google Scholar]
  26. Nicolas J. F., Avner P., Gaillard J., Guenet J. L., Jakob H., Jacob F. Cell lines derived from teratocarcinomas. Cancer Res. 1976 Nov;36(11 Pt 2):4224–4231. [PubMed] [Google Scholar]
  27. Nicolas J. F., Gaillard J., Jakob H., Jacob F. Bone-forming cell line derived from embryonal carcinoma cells. Nature. 1980 Aug 14;286(5774):716–718. doi: 10.1038/286716a0. [DOI] [PubMed] [Google Scholar]
  28. Nicolas J. F., Jakob H., Jacob F. Metabolic cooperation between mouse embryonal carcinoma cells and their differentiated derivatives. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3292–3296. doi: 10.1073/pnas.75.7.3292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Solter D., Knowles B. B. Immunosurgery of mouse blastocyst. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5099–5102. doi: 10.1073/pnas.72.12.5099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stevens L. C., Varnum D. S. The development of teratomas from parthenogenetically activated ovarian mouse eggs. Dev Biol. 1974 Apr;37(2):369–380. doi: 10.1016/0012-1606(74)90155-9. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES