Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1981 Jan 1;153(1):196–206. doi: 10.1084/jem.153.1.196

Role of immune recognition in latent allotype induction and clearance. Evidence for an allotypic network

PMCID: PMC2186050  PMID: 7452153

Abstract

The role of allotype recognition in the regulation of the expression of latent allotypes has been investigated in two series of experiments. The first experiments were designed to investigate the apparent instability of latent allotypes in circulation. In these experiments, clearance rates of IgG preparations bearing allotypes matched and unmatched to the recipient were examined. In all cases, iodinated IgG matched in allotype to the recipient was cleared at a normal rate from the serum. However, in several cases, iodinated IgG of an unmatched allotype was cleared at a rate and in a manner suggesting prior sensitization of the recipient to IgG of that allotype. Such apparent sensitization correlated with the presence of the foreign allotypes as a latent allotype in several bleedings taken both before and after the clearance experiment. In the second series of experiments, designed to test the ability of antiallotype antibodies to affect the expression of latent allotypes, five rabbits were immunized first with purified antiallotype antibodies and then after 3-4 mo, with streptococcal vaccine. Examination of the antistreptococcal antibodies for latent allotype revealed, in all cases, that the allotype against which the antiallotype antibodies were directed was present in levels 8- to 20- fold greater than were observed before the antiallotype injections. These results indicate that recognition of allotypic determinants is an important element in the control of latent allotype expression and suggest the existence of a regulatory network involving antiallotype antibodies.

Full Text

The Full Text of this article is available as a PDF (660.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aasted B., Sogn J. A., Kindt T. J. Isolation and characterization of rabbit antibodies directed against group a allotypic determinants. J Immunol. 1976 Feb;116(2):387–391. [PubMed] [Google Scholar]
  2. Bosma M. J., Bosma G. C. Congenic mouse strains: the expression of a hidden immunoglobulin allotype in a congenic partner strain of BALB-c mice. J Exp Med. 1974 Mar 1;139(3):512–527. doi: 10.1084/jem.139.3.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DIXON F. J., TALMAGE D. W., MAURER P. H., DEICHMILLER M. The half-life on homologous gamma globulin (antibody) in several species. J Exp Med. 1952 May;95(5):313–318. doi: 10.1084/jem.96.4.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Foppoli J. M., Wakeland E. K., Benedict A. A. Structural and genetic studies on chicken 7S immunoglobulin allotypes. IV. The presence of an unexpected chicken immunoglobulin heavy chain allotype: subclass or pseudoallele? J Immunol. 1978 Mar;120(3):812–817. [PubMed] [Google Scholar]
  5. Gilman-Sachs A., Dray S., Horng W. J. A common idiotypic specificity of rabbit antibodies to the a2 allotype of the immunoglobulin heavy chain variable region. J Immunol. 1980 Jul;125(1):96–101. [PubMed] [Google Scholar]
  6. Gottlieb A. B., Seide R. K., Kindt T. J. Quantitative determination of allotypic and idiotypic markers with antisera coupled to N-hydroxysuccinimide-activated sepharose. J Immunol. 1975 Jan;114(1 Pt 1):51–54. [PubMed] [Google Scholar]
  7. Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
  8. Krause R. M. The search for antibodies with molecular uniformity. Adv Immunol. 1970;12:1–56. doi: 10.1016/s0065-2776(08)60167-4. [DOI] [PubMed] [Google Scholar]
  9. Lobb N., Curtain C. C., Kidson C. Regulatory genes controlling the synthesis of heavy chains of human immunoglobulin-G. Nature. 1967 May 20;214(5090):783–785. doi: 10.1038/214783a0. [DOI] [PubMed] [Google Scholar]
  10. McCartney-Francis N., Mandy W. J. Serological and chemical studies of latent allotypes in the rabbit. Ann Immunol (Paris) 1979 Mar-Apr;130(2):115=31–115=31. [PubMed] [Google Scholar]
  11. Mudgett-Hunter M., Yarmush M. L., Fraser B. A., Kindt T. J. Rabbit latent group a allotypes: characterization and relationship to nominal group a allotypic specificities. J Immunol. 1978 Sep;121(3):1132–1138. [PubMed] [Google Scholar]
  12. Mudgett M., Fraser B. A., Kindt T. J. Nonallelic behavior of rabbit variable-region allotypes. J Exp Med. 1975 Jun 1;141(6):1448–1452. doi: 10.1084/jem.141.6.1448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Roland J., Cazenave P. A. Mise en évidence d'idiotypes anti-b4 apparentés chez les lapins de phénotype a 3+ immunisés contre l'allotype b4. C R Seances Acad Sci D. 1979 Feb 5;288(5):571–574. [PubMed] [Google Scholar]
  14. SPIEGELBERG H. L., WEIGLE W. O. THE CATABOLISM OF HOMOLOGOUS AND HETEROLOGOUS 7S GAMMA GLOBULIN FRAGMENTS. J Exp Med. 1965 Mar 1;121:323–338. doi: 10.1084/jem.121.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SVEHAG S. E., MANDEL B. THE FORMATION AND PROPERTIES OF POLIOVIRUS-NEUTRALIZING ANTIBODY. I. 19S AND 7S ANTIBODY FORMATION: DIFFERENCES IN KINETICS AND ANTIGEN DOSE REQUIREMENT FOR INDUCTION. J Exp Med. 1964 Jan 1;119:1–19. doi: 10.1084/jem.119.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spiegelberg H. L., Weigle W. O. Studies on the catabolism of gamma- G subunits and chains. J Immunol. 1965 Dec;95(6):1034–1040. [PubMed] [Google Scholar]
  17. Strosberg A. D., Hamers-Casterman C., Van der Loo W., Hamers R. A rabbit with the allotypic phenotype: ala2a3 b4b5b6. J Immunol. 1974 Oct;113(4):1313–1318. [PubMed] [Google Scholar]
  18. TALIAFERRO W. H., TALMAGE D. W. Antibodies in the rabbit with different rates of metabolic decay. J Infect Dis. 1956 Jul-Aug;99(1):21–33. doi: 10.1093/infdis/99.1.21. [DOI] [PubMed] [Google Scholar]
  19. Waldmann T. A., Strober W. Metabolism of immunoglobulins. Prog Allergy. 1969;13:1–110. doi: 10.1159/000385919. [DOI] [PubMed] [Google Scholar]
  20. Wolf B., Urban R., Miller A. B., Kimball E. S., Mudgett M., Catty D., Daneman J. Nonallelic inheritance of VH region alpha group allotypes: cell surface and serum studies in double and triple expressing rabbits. J Immunol. 1979 Oct;123(4):1858–1863. [PubMed] [Google Scholar]
  21. Yarmush M. L., Krutzsch H. C., Kindt T. J. Amino acid sequence analysis of immunoglobulin light chains by gas chromatographic-mass spectrometric techniques: structural identity of nominal and latent b9 molecules. Mol Immunol. 1980 Mar;17(3):319–326. doi: 10.1016/0161-5890(80)90052-8. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES