Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1981 Feb 1;153(2):437–449. doi: 10.1084/jem.153.2.437

Mechanisms of regulation of cell-mediated immunity. VII. Suppressor T cells induced by suboptimal doses of antigen plus an I-J-specific allogeneic effect

PMCID: PMC2186088  PMID: 6165798

Abstract

Intravenous injection of 0.01 mM 2,4,6-trinitrobenzene sulfonic acid- derivatized syngeneic lymphoid cells generates a Thy-1-positive, antigen-specific suppressor cell for contact sensitivity which requires an I-J allogeneic effect to become fully activated. It is necessary and sufficient for all allogeneic effect to be directed solely against the suppressor cell, and once activated, the cell can suppress in an H-2- unrestricted fashion. The results are discussed in the framework of entry into the suppressor pathway, the allogeneic effect as a reflection of normal physiologic processes, and the importance of I-J as a receptor and signal among cells in the suppressor pathway.

Full Text

The Full Text of this article is available as a PDF (814.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ciavarra R., Forman J. Cells treated with trinitrobenzene sulfonic acid express an antigenic determinant recognized by cytotoxic effector cells that is not detected on cells coated with trinitrophenylated proteins. J Immunol. 1980 Feb;124(2):713–718. [PubMed] [Google Scholar]
  2. Claman H. N., Miller S. D., Sy M. S. Suppressor cells in tolerance to contact sensitivity active against hapten-syngeneic and hapten-allogeneic determinants. J Exp Med. 1977 Jul 1;146(1):49–58. doi: 10.1084/jem.146.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Conlon P. J., Miller S. D., Claman H. N. The induction of tolerance to DNFB contact sensitivity by using hapten-modified lymphoid cells. III. Effects of hapten concentration on the ability of MLS-disparate cells to induce rapid unresponsiveness. J Immunol. 1980 Aug;125(2):807–813. [PubMed] [Google Scholar]
  4. Czitrom A. A., Sunshine G. H., Mitchison N. A. Suppression of the proliferative response to H-2D by I-J subregion gene products. Immunogenetics. 1980 Jul;11(1):97–102. doi: 10.1007/BF01567774. [DOI] [PubMed] [Google Scholar]
  5. Delovitch T. L., McDevitt H. O. In vitro analysis of allogeneic lymphocyte interaction. I. Characterization and cellular origin of an Ia-positive helper factor-allogeneic effect factor. J Exp Med. 1977 Oct 1;146(4):1019–1032. doi: 10.1084/jem.146.4.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Delovitch T. L., Sohn U. In vitro analysis of allogeneic lymphocyte interaction. III. Generation of a helper allogeneic effect factor (AEF) across an I-J subregion disparity. J Immunol. 1979 Apr;122(4):1528–1534. [PubMed] [Google Scholar]
  7. Delovitch T. L., Watson J., Battistella R., Harris J. F., Shaw J., Paetkau V. In vitro analysis of allogeneic lymphocyte interaction. V. Identification and characterization of two components of allogeneic effect factor, one of which displays H-2-restricted helper activity and the other, T cell-growth factor activity. J Exp Med. 1981 Jan 1;153(1):107–128. doi: 10.1084/jem.153.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eardley D. D., Hugenberger J., McVay-Boudreau L., Shen F. W., Gershon R. K., Cantor H. Immunoregulatory circuits among T-cell sets. I. T-helper cells induce other T-cell sets to exert feedback inhibition. J Exp Med. 1978 Apr 1;147(4):1106–1115. doi: 10.1084/jem.147.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eshhar Z., Armerding D., Waks T., Katz D. H. Activation of T and B lymphocytes in vitro. V. Cellular locus, metabolism and genetics of induction, and production of the allogeneic effect factor. J Immunol. 1977 Oct;119(4):1457–1467. [PubMed] [Google Scholar]
  10. Finberg R., Burakoff S. J., Benacerraf B., Greene M. I. The cytolytic T lymphocyte response to trinitrophenyl-modified syngeneic cells. II. Evidence for antigen-specific suppressor T cells. J Immunol. 1979 Sep;123(3):1210–1214. [PubMed] [Google Scholar]
  11. Forman J., Vitetta E. S., Hart D. A. Relationship between trinitrophenyl and H-2 antigens on trinitrophenyl-modified spleen cells. II. Correlation between derivatization of H-2 antigens with trinitrophenyl and the ability of trinitrophenyl-modified cells to react functionally to the CML assay. J Immunol. 1977 Mar;118(3):803–808. [PubMed] [Google Scholar]
  12. Herzenberg L. A., Black S. J., Herzenberg L. A. Regulatory circuits and antibody responses. Eur J Immunol. 1980 Jan;10(1):1–11. doi: 10.1002/eji.1830100102. [DOI] [PubMed] [Google Scholar]
  13. Herzenberg L. A., Okumura K., Cantor H., Sato V. L., Shen F. W., Boyse E. A., Herzenberg L. A. T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells. J Exp Med. 1976 Aug 1;144(2):330–344. doi: 10.1084/jem.144.2.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Katz D. H., Paul W. E., Goidl E. A., Benacerraf B. Carrier function in anti-hapten antibody responses. 3. Stimulation of antibody synthesis and facilitation of hapten-specific secondary antibody responses by graft-versus-host reactions. J Exp Med. 1971 Feb 1;133(2):169–186. doi: 10.1084/jem.133.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Katz D. H. The allogeneic effect on immune responses: model for regulatory influences of T lymphocytes on the immune system. Transplant Rev. 1972;12:141–179. doi: 10.1111/j.1600-065x.1972.tb00055.x. [DOI] [PubMed] [Google Scholar]
  16. Meruelo D., Flieger N., Smith D., McDevitt H. O. In vivo or in vitro treatments with anti-I-J alloantisera abolish immunity to AKR leukemia. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2178–2182. doi: 10.1073/pnas.77.4.2178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miller S. D. Suppressor T-cell mechanisms in contact sensitivity. III. Apparent non-major histocompatibility complex restriction is a result of multiple sets of major histocompatibility complex-specific suppressor T cells induced by syngeneic 2,4-dinitrophenyl-modified lymphoid cells. J Exp Med. 1979 Sep 19;150(3):676–692. doi: 10.1084/jem.150.3.676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Niederhuber J. E., Allen P. Role of I-region gene products in macrophage induction of an antibody response. II. Restriction at the level of T cell in recognition of I-J-subregion macrophage determinants. J Exp Med. 1980 May 1;151(5):1103–1113. doi: 10.1084/jem.151.5.1103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pierres A., Bromberg J. S., Sy M. S., Benacerraf B., Greene M. I. Mechanisms of regulation of cell-mediated immunity. VI. Antigen density dependence of the induction of genetically restricted suppressor cells. J Immunol. 1980 Jan;124(1):343–348. [PubMed] [Google Scholar]
  20. Rohrer J. W., Odermatt B., Lynch R. G. Immunoregulation of murine myeloma: isologous immunization with M315 induces idiotype-specific T cells that suppress IgA secretion by MOPC-315 cells in vivo. J Immunol. 1979 May;122(5):2011–2019. [PubMed] [Google Scholar]
  21. Streilein J. W., Klein J. Neonatal tolerance of H-2 alloantigens. I. I region modulation of tolerance potential of K and D antigens. Proc R Soc Lond B Biol Sci. 1980 Apr 22;207(1169):461–474. doi: 10.1098/rspb.1980.0034. [DOI] [PubMed] [Google Scholar]
  22. Streilein J. W. Neonatal tolerance of H-2 alloantigens. II. I region dependence of tolerance expressed to K and D antigens. Proc R Soc Lond B Biol Sci. 1980 Apr 22;207(1169):475–486. doi: 10.1098/rspb.1980.0035. [DOI] [PubMed] [Google Scholar]
  23. Sy M. S., Dietz M. H., Germain R. N., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. IV. Idiotype-bearing I-J+ suppressor T cell factors induce second-order suppressor T cells which express anti-idiotypic receptors. J Exp Med. 1980 May 1;151(5):1183–1195. doi: 10.1084/jem.151.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tada T., Takemori T., Okumura K., Nonaka M., Tokuhisa T. Two distinct types of helper T cells involved in the secondary antibody response: independent and synergistic effects of Ia- and Ia+ helper T cells. J Exp Med. 1978 Feb 1;147(2):446–458. doi: 10.1084/jem.147.2.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Waltenbaugh C., Thèze J., Kapp J. A., Benacerraf B. Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyrosine50 (GT). III. Generation of suppressor T cells by a suppressive extract derived from GT-primed lymphoid cells. J Exp Med. 1977 Oct 1;146(4):970–985. doi: 10.1084/jem.146.4.970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weinberger J. Z., Germain R. N., Benacerraf B., Dorf M. E. Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. V. Role of idiotypes in the suppressor pathway. J Exp Med. 1980 Jul 1;152(1):161–169. doi: 10.1084/jem.152.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. van Rood J. J., Persijn G. G., van Leeuwen A., Goulmy E., Gabb B. W. A new strategy to improve kidney graft survival: the induction of CML nonresponsiveness. Transplant Proc. 1979 Mar;11(1):736–742. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES