Abstract
This study was directed at surface structures that are found on human lymphohemopoietic progenitor cells in normal and leukemic bone marrow. A monoclonal antibody was produced against an acute lymphoblastic leukemia (ALL) cell line of the pre-B phenotype; this antibody (BA-2) was used to demonstrate a cell surface polypeptide of approximately 24,000 mol wt that migrates similarly in both reduced and nonreduced form. This polypeptide, p24/BA-2, was shown by immune precipitation and gel electrophoresis and cell distribution studies to be different from HLA-DR and gp 100/cALLa. p24/BA-2 was present on the surface of 77% (54/70) of cases of non-T, non-B ALL; BA-2 staining was less bright or nondetectable in surface Ig+ (SIg+) chronic lymphocytic leukemia (CLL) and T ALL and nondetectable on peripheral T and B lymphocytes. Approximately 3% of bone marrow mononuclear cells were p24/BA-2+, and these cells were E rosette-, surface (SIg-), and nonphagocytic. Marrow TdT+ progenitor cells were frequently p 24/BA-2+. Results suggest that p24/BA-2 represents a surface structure present on lymphohemopoietic bone marrow progenitor cells and that most common types of ALL bear the p25/BA-2 structure.
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Selected References
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- Abramson C. S., Kersey J. H., LeBien T. W. A monoclonal antibody (BA-1) reactive with cells of human B lymphocyte lineage. J Immunol. 1981 Jan;126(1):83–88. [PubMed] [Google Scholar]
- Ades E. W., Dougherty P. A., Balch C. M. Partial immunochemical characterization of human B-lymphocyte differentiation antigen (BDA-1). Adv Exp Med Biol. 1979;121B:239–246. doi: 10.1007/978-1-4684-8914-9_22. [DOI] [PubMed] [Google Scholar]
- Ades E. W., Zwerner R. K., Acton R. T., Balch C. M. Isolation and partial characterization of the human homologue of Thy-1. J Exp Med. 1980 Feb 1;151(2):400–406. doi: 10.1084/jem.151.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berntorp E., Turesson I., Zettervall O. Heterologous B-cell antisera may detect non-Ig, non-HLA-DR antigens. Scand J Immunol. 1979;10(1):17–24. doi: 10.1111/j.1365-3083.1979.tb01329.x. [DOI] [PubMed] [Google Scholar]
- Brodsky F. M., Parham P., Barnstable C. J., Crumpton M. J., Bodmer W. F. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979;47:3–61. doi: 10.1111/j.1600-065x.1979.tb00288.x. [DOI] [PubMed] [Google Scholar]
- Brouet J. C., Preud'homme J. L., Penit C., Valensi F., Rouget P., Seligmann M. Acute lymphoblastic leukemia with pre-B-cell characteristics. Blood. 1979 Jul;54(1):269–273. [PubMed] [Google Scholar]
- Brown G., Capellaro D., Greaves M. Leukemia-associated antigens in man. J Natl Cancer Inst. 1975 Dec;55(6):1281–1289. doi: 10.1093/jnci/55.6.1281. [DOI] [PubMed] [Google Scholar]
- Fu S. M., Winchester R. J., Kunkel H. G. The occurrence of the HL-B alloantigens on the cells of unclassified acute lymphoblastic leukemias. J Exp Med. 1975 Nov 1;142(5):1334–1338. doi: 10.1084/jem.142.5.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greaves M., Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim Biophys Acta. 1978 Oct 27;516(2):193–230. doi: 10.1016/0304-419x(78)90008-2. [DOI] [PubMed] [Google Scholar]
- Hurwitz R., Hozier J., LeBien T., Minowada J., Gajl-Peczalska K., Kubonishi I., Kersey J. Characterization of a leukemic cell line of the pre-B phenotype. Int J Cancer. 1979 Feb;23(2):174–180. doi: 10.1002/ijc.2910230206. [DOI] [PubMed] [Google Scholar]
- Janossy G., Bollum F. J., Bradstock K. F., McMichael A., Rapson N., Greaves M. F. Terminal transferase-positive human bone marrow cells exhibit the antigenic phenotype of common acute lymphoblastic leukemia. J Immunol. 1979 Oct;123(4):1525–1529. [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- LeBien T. W., Kersey J. H. A monoclonal antibody (TA-1) reactive with human T lymphocytes and monocytes. J Immunol. 1980 Nov;125(5):2208–2214. [PubMed] [Google Scholar]
- McKenzie I. F., Potter T. Murine lymphocyte surface antigens. Adv Immunol. 1979;27:179–338. doi: 10.1016/s0065-2776(08)60263-1. [DOI] [PubMed] [Google Scholar]
- Minowada J., Janossy G., Greaves M. F., Tsubota T., Srivastava B. I., Morikawa S., Tatsumi E. Expression of an antigen associated with acute lymphoblastic leukemia in human leukemia-lymphoma cell lines. J Natl Cancer Inst. 1978 Jun;60(6):1269–1277. doi: 10.1093/jnci/60.6.1269. [DOI] [PubMed] [Google Scholar]
- Niaudet P., Greaves M. Diversity of cell surface polypeptides that are recognized by heteroantisera to human T cells. J Immunol. 1980 Mar;124(3):1203–1208. [PubMed] [Google Scholar]
- Ritz J., Pesando J. M., Notis-McConarty J., Lazarus H., Schlossman S. F. A monoclonal antibody to human acute lymphoblastic leukaemia antigen. Nature. 1980 Feb 7;283(5747):583–585. doi: 10.1038/283583a0. [DOI] [PubMed] [Google Scholar]
- Schlossman S. F., Chess L., Humphreys R. E., Strominger J. L. Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288–1292. doi: 10.1073/pnas.73.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogler L. B., Crist W. M., Bockman D. E., Pearl E. R., Lawton A. R., Cooper M. D. Pre-B-cell leukemia. A new phenotype of childhood lymphoblastic leukemia. N Engl J Med. 1978 Apr 20;298(16):872–878. doi: 10.1056/NEJM197804202981603. [DOI] [PubMed] [Google Scholar]
- Wang C. Y., Fu S. M., Kunkel H. G. Isolation and immunological characterization of a major surface glycoprotein (gp54) preferentially expressed on certain human B cells. J Exp Med. 1979 Jun 1;149(6):1424–1437. doi: 10.1084/jem.149.6.1424. [DOI] [PMC free article] [PubMed] [Google Scholar]