Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1981 Apr 1;153(4):992–997. doi: 10.1084/jem.153.4.992

Effector T lymphocytes in lymphocytic choriomeningitis virus-infected mice. Cytolytic activity of Lyt-23 spleen cells in vitro does not correlate with elimination of infectious virus from spleens

PMCID: PMC2186119  PMID: 6972995

Abstract

The T lymphocytes from mice recovering from infection with lymphocytic choriomeningitis virus were selected for subclasses by treatment with anti-Lyt antisera and complement. Lyt-23 cells and mixtures of lyt-1 and Lyt-23 cells caused up to one-half the destruction of cultivated target cells as compared with untreated T lymphocytes; Lyt-1 cells alone were not cytotoxic. Selected and unselected spleen T cells were also inoculated intravenously into previously infected mice. Whereas unselected cells reduced infectious virus in the spleens of the recipients approximately 100-fold, only marginal effects, which were not preferentially associated with one particular subclass, were seen with selected LYT-23 or Lyt-1 lymphocytes or a mixture of both. Apparently the Lyt-23 cells, shown to by cytolytic for infected cells in vitro, did not cause elimination of a measurable quantity of the virus from mice.

Full Text

The Full Text of this article is available as a PDF (358.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanden R. V., Bowern N. A., Pang T. E., Gardner I. D., Parish C. R. Effects of thymus-independent (B) cells and the H-2 gene complex on antiviral function of immune thymus-derived (T) cells. Aust J Exp Biol Med Sci. 1975 Jun;53(3):187–195. doi: 10.1038/icb.1975.19. [DOI] [PubMed] [Google Scholar]
  2. Hoffsten P. E., Oldstone M. B., Dixon F. J. Immunopathology of adoptive immunization in mice chronically infected with lymphocytic choriomeningitis virus. Clin Immunol Immunopathol. 1977 Jan;7(1):44–52. doi: 10.1016/0090-1229(77)90028-9. [DOI] [PubMed] [Google Scholar]
  3. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  4. LEHMANN-GRUBE F. LYMPHOCYTIC CHORIOMENINGITIS IN THE MOUSE. I. GROWTH IN THE BRAIN. Arch Gesamte Virusforsch. 1964;14:344–350. doi: 10.1007/BF01555827. [DOI] [PubMed] [Google Scholar]
  5. Lehmann-Grube F., Ambrassat J. A new method to detect lymphocytic choriomeningitis virus-specific antibody in human sera. J Gen Virol. 1977 Oct;37(1):85–92. doi: 10.1099/0022-1317-37-1-85. [DOI] [PubMed] [Google Scholar]
  6. Popescu M., Lehmann-Grube F. Diversity of lymphocytic choriomeningitis virus: variation due to replication of the virus in the mouse. J Gen Virol. 1976 Jan;30(1):113–122. doi: 10.1099/0022-1317-30-1-113. [DOI] [PubMed] [Google Scholar]
  7. Rivers T. M., Scott T. F. MENINGITIS IN MAN CAUSED BY A FILTERABLE VIRUS : II. IDENTIFICATION OF THE ETIOLOGICAL AGENT. J Exp Med. 1936 Feb 29;63(3):415–432. doi: 10.1084/jem.63.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Simon M. M., Abenhardt B. Generation of effector cells from T cell subsets. II. Lyt 123 T cells contain the precursors for all primary cytotoxic effector cells and for cells involved in the regulation of cytotoxic responses. Eur J Immunol. 1980 May;10(5):334–341. doi: 10.1002/eji.1830100504. [DOI] [PubMed] [Google Scholar]
  9. Volkert M., Larsen J. H. Immunological tolerance to viruses. Prog Med Virol. 1965;7:160–207. [PubMed] [Google Scholar]
  10. Yap K. L., Ada G. L., McKenzie I. F. Transfer of specific cytotoxic T lymphocytes protects mice inoculated with influenza virus. Nature. 1978 May 18;273(5659):238–239. doi: 10.1038/273238a0. [DOI] [PubMed] [Google Scholar]
  11. Zinkernagel R. M., Doherty P. C. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv Immunol. 1979;27:51–177. doi: 10.1016/s0065-2776(08)60262-x. [DOI] [PubMed] [Google Scholar]
  12. Zinkernagel R. M., Welsh R. M. H-2 compatibility requirement for virus-specific T cell-mediated effector functions in vivo. I. Specificity of T cells conferring antiviral protection against lymphocytic choriomeningitis virus is associated with H-2K and H-2D. J Immunol. 1976 Nov;117(5 Pt 1):1495–1502. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES