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. 1981 Apr 1;153(4):924–935. doi: 10.1084/jem.153.4.924

Generation of idiotype-specific T cell help through network perturbation

PMCID: PMC2186126  PMID: 6972994

Abstract

Different manipulations of BALB/c mice were used to generate idiotype- specific help: neonatally induced suppression of the T 15 idiotype and low-dose priming with anti-T15 antibody. The splenic foci culture system was used to study T15-idiotype-recognizing helper T cells under limiting-cell-dose conditions. These treatments induced T15 idiotype- specific help for B cells responding to TNP-T15. Normal or hemocyanin- primed BALB/c mice did not supply T15 idiotype-specific help. The helper cells were sensitive to anti-Thy-1.2 and complement treatment and can distinguish T15 from an idiotype-different, PC-binding myeloma protein, M167, and the TNP binding myeloma protein, M460. These data show that idiotype-specific T helper cells can be induced by at least two different manipulations of the idiotype network. These manipulations presumably do not act directly on the T15-recognizing T cells, but must involve complementary idiotypic circuits that stimulate anti-T15 specific T cells. Furthermore, this study demonstrates that the splenic-fragment culture technique provides a general method to investigate, at the single cell level, idiotypic T-B cell interactions induced by perturbations of the immune network.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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