Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1981 Apr 1;153(4):936–950. doi: 10.1084/jem.153.4.936

Structure of murine Ia antigens. Two dimensional electrophoretic analyses and high pressure liquid chromatography tryptic peptide maps of products of the I-A and I-E subregions and of an associated invariant polypeptide

PMCID: PMC2186129  PMID: 6942091

Abstract

We demonstrate that an invariant polypeptide, first described by Jones et al. (21), co-immunoprecipitates with our Ia molecules, that its interaction with Ia polypeptides varies with haplotype, and that it is not a precursor of the Aalpha, Abeta, Ealpha, or Ebeta. polypeptides. We also show that the polypeptides that we have previously characterized are contaminated with very little, if any, invariant protein. Further, we have used our high-pressure liquid chromatography tryptic peptide map technique to formally map the genes encoding Aalpha, Abeta, and Ebeta to the I-A subregion using recombinant and F1 hybrid mice.

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison J. P., Walker L. E., Russell W. A., Pellegrino M. A., Ferrone S., Reisfeld R. A., Frelinger J. A., Silver J. Murine Ia and human DR antigens: homology of amino-terminal sequences. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3953–3956. doi: 10.1073/pnas.75.8.3953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benacerraf B., Germain R. N. The immune response genes of the major histocompatibility complex. Immunol Rev. 1978;38:70–119. doi: 10.1111/j.1600-065x.1978.tb00385.x. [DOI] [PubMed] [Google Scholar]
  3. Cecka J. M., Mc Millan M., Murphy D. B., McDevitt H. O., Hood L. Partial N-terminal amino acid sequence analyses and comparative tryptic peptide maps of murine Ia molecules encoded by the I-A subregion. Eur J Immunol. 1979 Dec;9(12):955–963. doi: 10.1002/eji.1830091209. [DOI] [PubMed] [Google Scholar]
  4. Cook R. G., Capra J. D., Bednarczyk J. L., Uhr J. W., Vitetta E. S. Structural studies on the murine Ia alloantigens. VI. Evidence that both subunits of the I-A alloantigen are encoded by the I-A subregion. J Immunol. 1979 Dec;123(6):2799–2803. [PubMed] [Google Scholar]
  5. Cook R. G., Siegelman M. H., Capra J. D., Uhr J. W., Vitetta E. S. Structural studies on the murine Ia alloantigens. IV. NH2-terminal sequence analysis of allelic products of the I-A and I-E/C subregions. J Immunol. 1979 Jun;122(6):2232–2237. [PubMed] [Google Scholar]
  6. Cook R. G., Vitetta E. S., Uhr J. W., Capra J. D. Structural studies on the murine Ia alloantigens--III. Tryptic peptide comparisons of allelic products of the I-E/C sub-region. Mol Immunol. 1979 Jan;16(1):29–35. doi: 10.1016/0161-5890(79)90024-5. [DOI] [PubMed] [Google Scholar]
  7. Cook R. G., Vitetta E. S., Uhr J. W., Capra J. D. Structural studies on the murine Ia alloantigens. V. Evidence that the structural gene for the I-E/C beta polypeptide is encoded within the I-A subregion. J Exp Med. 1979 Apr 1;149(4):981–986. doi: 10.1084/jem.149.4.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cook R. G., Vitetta E. S., Uhr J. W., Capra J. D. Structural studies on the murine Ia alloantigens. VII. Further evidence for two-gene control of the E/C alloantigens. J Immunol. 1980 Apr;124(4):1594–1598. [PubMed] [Google Scholar]
  9. Cullen S. E., Schwartz B. D. An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci. J Immunol. 1976 Jul;117(1):136–142. [PubMed] [Google Scholar]
  10. Delovitch T. L., Barber B. H. Evidence for two homologous, but nonidentical, Ia molecules determined by the I-EC subregion. J Exp Med. 1979 Jul 1;150(1):100–107. doi: 10.1084/jem.150.1.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Emerson S. G., Murphy D. B., Cone R. E. Ia antigen turnover. II. The kinetics of biosynthesis and release of Ia alpha- and beta-chains by murine spleen cells in culture. J Immunol. 1980 Jul;125(1):406–410. [PubMed] [Google Scholar]
  12. Freed J. H., David C. S., Shreffler D. C., Nathenson S. G. Structural studies of the protein portion of the H-2-linked Ia glycoprotein antigens of the mouse: tryptic peptide comparison of products from the I-A and I-C subregions of B10-HTT. J Immunol. 1978 Jul;121(1):91–97. [PubMed] [Google Scholar]
  13. Frelinger J. A., Niederhuber J. E., Shreffler D. C. Inhibition of immune responses in vitro by specific antiserums to Ia antigens. Science. 1975 Apr 18;188(4185):268–270. doi: 10.1126/science.1118728. [DOI] [PubMed] [Google Scholar]
  14. Hayes C. E., Bach F. H. I-N: a newly described H-2 I subregion between K and I-A. J Exp Med. 1980 Feb 1;151(2):481–485. doi: 10.1084/jem.151.2.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jones P. P. Analysis of H-2 and Ia molecules by two-dimensional gel electrophoresis. J Exp Med. 1977 Nov 1;146(5):1261–1279. doi: 10.1084/jem.146.5.1261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jones P. P., Murphy D. B., Hewgill D., McDevitt H. O. Detection of a common polypeptide chain in I--A and I--E sub-region immunoprecipitates. Mol Immunol. 1979 Jan;16(1):51–60. doi: 10.1016/0161-5890(79)90027-0. [DOI] [PubMed] [Google Scholar]
  17. Jones P. P., Murphy D. B., McDevitt H. O. Two-gene control of the expression of a murine Ia antigen. J Exp Med. 1978 Oct 1;148(4):925–939. doi: 10.1084/jem.148.4.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  19. Lafuse W. P., McCormick J. F., David C. S. Serological and biochemical identification of hybrid Ia antigens. J Exp Med. 1980 Mar 1;151(3):709–715. doi: 10.1084/jem.151.3.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  21. Lerner E. A., Matis L. A., Janeway C. A., Jr, Jones P. P., Schwartz R. H., Murphy D. B. Monoclonal antibody against an Ir gene product? J Exp Med. 1980 Oct 1;152(4):1085–1101. doi: 10.1084/jem.152.4.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. McMillan M., Cecka J. M., Hood L., Murphy D. B., McDevitt H. O. Peptide map analyses of murine Ia antigens of the I-E subregion using HPLC. Nature. 1979 Feb 22;277(5698):663–665. doi: 10.1038/277663a0. [DOI] [PubMed] [Google Scholar]
  23. McMillan M., Cecka J. M., Murphy D. B., McDevitt H. O., Hood L. Structure of murine Ia antigens: partial NH2-terminal amino acid sequences of products of the I-E or I-C subregion. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5135–5139. doi: 10.1073/pnas.74.11.5135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moosic J. P., Nilson A., Hämmerling G. J., McKean D. J. Biochemical characterization of Ia antigens. I. Characterization of the 31K polypeptide associated with I-A subregion Ia antigens. J Immunol. 1980 Oct;125(4):1463–1469. [PubMed] [Google Scholar]
  25. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  26. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  27. Sachs D. H., Cone J. L. A mouse B-cell alloantigen determined by gene(s) linked to the major histocompatibility complex. J Exp Med. 1973 Dec 1;138(6):1289–1304. doi: 10.1084/jem.138.6.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schwartz R. H., David C. S., Sachs D. H., Paul W. E. T lymphocyte-enriched murine peritoneal exudate cells. III. Inhibition of antigen-induced T lymphocyte Proliferation with anti-Ia antisera. J Immunol. 1976 Aug;117(2):531–540. [PubMed] [Google Scholar]
  29. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  30. Silver J., Russell W. A., Reis B. L., Frelinger J. A. Chemical characterization of murine Ia alloantigens determined by the i-E/i-C subregions of the H-2 complex. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5131–5134. doi: 10.1073/pnas.74.11.5131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Silver J., Russell W. A. Structural polymorphism of I-E subregion antigens determined by a gene in the H-2K to I-B genetic interval. Nature. 1979 May 31;279(5712):437–439. doi: 10.1038/279437a0. [DOI] [PubMed] [Google Scholar]
  32. Silver J. Trans gene complementation of I-E subregion antigens. J Immunol. 1979 Sep;123(3):1423–1425. [PubMed] [Google Scholar]
  33. Silver J., Walker L. E., Reisfeld R. A., Pellegrino M. A., Ferrone S. Structural studies of murine I-E and human DR antigens. Mol Immunol. 1979 Jan;16(1):37–42. doi: 10.1016/0161-5890(79)90025-7. [DOI] [PubMed] [Google Scholar]
  34. Springer T. A., Kaufman J. F., Terhorst C., Strominger J. L. Purification and structural characterisation of human HLA-linked B-cell antigens. Nature. 1977 Jul 21;268(5617):213–218. doi: 10.1038/268213a0. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES