Abstract
Employing a new method for allogeneic bone marrow transplantation, irradiation chimeras constructed from various combinations of marrow cells from B10 H-2 recombinant mice and AKR recipients were prepared. Though these chimeras had well-developed populations of T and B cells, they showed strikingly different patterns of responses in the primary antibody formation to sheep erythrocytes (SRBC), a T dependent antigen. These are (a) AKR mice treated with C57BL/10 cells, [B10 leads to AKR] fully H-2 incompatible, and AKR mice treated with B10.A (5R) cells, [5R leads to AKR] I-J,E compatible chimeras that were almost completely unresponsive to SRBC; (b) AKR mice treated with B10.BR cells [BR leads to AKR] fully H-2 compatible, and AKR mice treated with B10 AKM cells, [AKM leads to AKR] chimeras where donor and recipient differed only at H-2D, showed the same number of plaque-forming cells (PFC) as B10 control mice; (c) AKR mice treated with B10.A cells, [B10 leads to AKR] chimeras, where donor and recipient were matched at H-2K-I-E region, showed about one-half the number of PFC as the control mice. From these results we conclude that in allogeneic bone marrow chimeras primary antibody response to T-dependent antigen, such as SRBC, is generated when at least the K end of the H-2 complex is compatible between donor and recipient.
Full Text
The Full Text of this article is available as a PDF (478.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bevan M. J. In a radiation chimaera, host H-2 antigens determine immune responsiveness of donor cytotoxic cells. Nature. 1977 Sep 29;269(5627):417–418. doi: 10.1038/269417a0. [DOI] [PubMed] [Google Scholar]
- Fernandes G., Nair M., Onoe K., Tanaka T., Floyd R., Good R. A. Impairment of cell-mediated immunity functions by dietary zinc deficiency in mice. Proc Natl Acad Sci U S A. 1979 Jan;76(1):457–461. doi: 10.1073/pnas.76.1.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katz D. H., Benacerraf B. The function and interrelationships of T-cell receptors, Ir genes and other histocompatibility gene products. Transplant Rev. 1975;22:175–195. doi: 10.1111/j.1600-065x.1975.tb01559.x. [DOI] [PubMed] [Google Scholar]
- Katz D. H., Katz L. R., Bogowitz C. A., Skidmore B. J. Adaptive differentiation of murine lymphocytes. II. The thymic microenvironment does not restrict the cooperative partner cell preference of helper T cells differentiating in F1 leads to F1 thymic chimeras. J Exp Med. 1979 Jun 1;149(6):1360–1370. doi: 10.1084/jem.149.6.1360. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nordin A. A., Farrar J. J. Studies of the immunological capacity of germfree mouse radiation chimeras. III. In vitro reconstitution of the T-helper cell deficiency. Cell Immunol. 1974 Feb;10(2):218–225. doi: 10.1016/0008-8749(74)90113-0. [DOI] [PubMed] [Google Scholar]
- Onoé K., Fernandes G., Good R. A. Humoral and cell-mediated immune responses in fully allogeneic bone marrow chimera in mice. J Exp Med. 1980 Jan 1;151(1):115–132. doi: 10.1084/jem.151.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sado T., Kamisaku H. Histocompatibility and T-B cell cooperation in mouse radiation chimeras. J Immunol. 1975 Dec;115(6):1607–1612. [PubMed] [Google Scholar]
- Smith F. I., Miller J. F. Suppression of T cells specific for the nonthymic parental H-2 haplotype in thymus-grafted chimeras. J Exp Med. 1980 Jan 1;151(1):246–251. doi: 10.1084/jem.151.1.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith F. I., Mottram P. L., Miller J. F. I-region-coded products expressed on both macrophages and thymus epithelium influence T-cell activities. Scand J Immunol. 1979;10(4):343–347. doi: 10.1111/j.1365-3083.1979.tb01361.x. [DOI] [PubMed] [Google Scholar]
- Sprent J. Restricted helper function of F1 leads to parent bone marrow chimeras controlled by K-end of H-2 complex. J Exp Med. 1978 Jun 1;147(6):1838–1842. doi: 10.1084/jem.147.6.1838. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trier L., Rubin B. Bone marrow transplantation in inbred strains of mice. I. The failure of development of normal T cell function following allogeneic transplantation. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Oct;82B(5):724–732. [PubMed] [Google Scholar]
- Urso P., Gengozian N. Variation in T and B cell deficiency in different mouse allogeneic radiation chimeras. J Immunol. 1974 Dec;113(6):1770–1779. [PubMed] [Google Scholar]
- Williamson A. R. Three-receptor, clonal expansion model for selection of self-recognition in the thymus. Nature. 1980 Feb 7;283(5747):527–532. doi: 10.1038/283527a0. [DOI] [PubMed] [Google Scholar]
- Zinkernagel R. M., Callahan G. N., Althage A., Cooper S., Klein P. A., Klein J. On the thymus in the differentiation of "H-2 self-recognition" by T cells: evidence for dual recognition? J Exp Med. 1978 Mar 1;147(3):882–896. doi: 10.1084/jem.147.3.882. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zinkernagel R. M. Thymus and lymphohemopoietic cells: their role in T cell maturation in selection of T cells' H-2-restriction-specificity and in H-2 linked Ir gene control. Immunol Rev. 1978;42:224–270. doi: 10.1111/j.1600-065x.1978.tb00264.x. [DOI] [PubMed] [Google Scholar]