Abstract
The high-potential 4Fe-4S* center ofAzotobacter vinelandii ferredoxin I has been titrated potentiometrically by a reductive procedure. The absorbance decrease at 510 nm accompanying the reduction of the high-potential center titrated with an Em of 320 mV (n = 1). The low-potential 4Fe-4S* center was titrated by using the absorbance decrease at 410 nm to monitor its reduction. This center exhibited an Em of -424 mV (n = 1).
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- Dutton P. L. Oxidation-reduction potential dependence of the interaction of cytochromes, bacteriochlorophyll and carotenoids at 77 degrees K in chromatophores of Chromatium D and Rhodopseudomonas gelatinosa. Biochim Biophys Acta. 1971 Jan 12;226(1):63–80. doi: 10.1016/0005-2728(71)90178-2. [DOI] [PubMed] [Google Scholar]
- Howard J. B., Lorsbach T., Que L. Iron-sulfur clusters and cysteine distribution in a ferredoxin from Azotobacter vinelandii. Biochem Biophys Res Commun. 1976 May 17;70(2):582–588. doi: 10.1016/0006-291x(76)91087-1. [DOI] [PubMed] [Google Scholar]
- Sweeney W. V., Rabinowitz J. C., Yoch D. C. High and low reduction potential 4Fe-4S clusters in Azotobacter vinelandii (4Fe-4S) 2ferredoxin I. Influence of the polypeptide on the reduction potentials. J Biol Chem. 1975 Oct 10;250(19):7842–7847. [PubMed] [Google Scholar]
- Yoch D. C., Arnon D. I. Two biologically active ferredoxins from the aerobic nitrogen-fixing bacteriu, Azotobacter vinelandii. J Biol Chem. 1972 Jul 25;247(14):4514–4520. [PubMed] [Google Scholar]
- Yoch D. C., Benemann J. R., Valentine R. C., Arnon D. I. The electron transport system in nitrogen fixation by Azotobacter. II. Isolation and function of a new type of ferredoxin. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1404–1410. doi: 10.1073/pnas.64.4.1404. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoch D. C., Carithers R. P., Arnon D. I. Isolation and characterization of bound ion-sulfur proteins from bacterial photosynthetic membranes. I. Ferredoxins III and IV from Rhodospirillum rubrum chromatophores. J Biol Chem. 1977 Nov 10;252(21):7453–7460. [PubMed] [Google Scholar]