Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1981 May 1;153(5):1161–1172. doi: 10.1084/jem.153.5.1161

Enhancement of t lymphocyte functions by Fc fragments of immunoglobulins. I. Augmentation of allogeneic mixed lymphocyte culture reactions requires I-A- or I-B-subregion differences between effector and stimulator cell populations

EL Morgan, ML Thoman, WO Weigle
PMCID: PMC2186150  PMID: 6454744

Abstract

Fc fragments derived from human Ig were found to be capable of enhancing T cell-mediated, antigen-induced proliferative and mixed lymphocyte culture responses. Maximum enhancement occurred when suboptimal amounts of antigen or suboptimal numbers of stimulator cells were employed. Augmentation of the allogeneic mixed lymphocyte culture reaction requires an I-A and/or I-B subregion difference between effector and stimulator cell populations. Although a significant proliferative response was observed with K- or D- region differences, Fc fragments were unable to enhance the response. The T cell population acted upon by Fc fragments in the potentiation of these responses bears the Lyt-1(+)23(-) phenotype.

Full Text

The Full Text of this article is available as a PDF (658.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alter B. J., Bach F. H. Role of H-2 lymphocyte-defined and serologically-defined components in the generation of cytotoxic lymphocytes. J Exp Med. 1974 Nov 1;140(5):1410–1415. doi: 10.1084/jem.140.5.1410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bach F. H., Alter B. J. Alternative pathways of T lymphocyte activation. J Exp Med. 1978 Sep 1;148(3):829–834. doi: 10.1084/jem.148.3.829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bach F. H., Bach M. L., Sondel P. M. Differential function of major histocompatibility complex antigens in T-lymphocyte activation. Nature. 1976 Jan 29;259(5541):273–281. doi: 10.1038/259273a0. [DOI] [PubMed] [Google Scholar]
  4. Berman M. A., Weigle W. O. B-lymphocytes activation by the Fc region of IgG. J Exp Med. 1977 Jul 1;146(1):241–256. doi: 10.1084/jem.146.1.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cantor H., Boyse E. A. Regulation of cellular and humoral immune responses by T-cell subclasses. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):23–32. doi: 10.1101/sqb.1977.041.01.006. [DOI] [PubMed] [Google Scholar]
  8. Corradin G., Etlinger H. M., Chiller J. M. Lymphocyte specificity to protein antigens. I. Characterization of the antigen-induced in vitro T cell-dependent proliferative response with lymph node cells from primed mice. J Immunol. 1977 Sep;119(3):1048–1053. [PubMed] [Google Scholar]
  9. Hayes C. E., Bach F. H. I-N: a newly described H-2 I subregion between K and I-A. J Exp Med. 1980 Feb 1;151(2):481–485. doi: 10.1084/jem.151.2.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  11. Morgan E. L., Thoman M. L., Walker S. M., Weigle W. O. Regulation of the immune response. II. Characterization of the cell population(s) involved in the Fc fragment-induced adjuvant effect. J Immunol. 1980 Sep;125(3):1275–1279. [PubMed] [Google Scholar]
  12. Morgan E. L., Walker S. M., Thoman M. L., Weigle W. O. Regulation of the immune response. I. The potentiation of in vivo and in vitro immune responses by Fc fragments. J Exp Med. 1980 Jul 1;152(1):113–123. doi: 10.1084/jem.152.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morgan E. L., Weigle W. O. Polyclonal activation of murine B lymphocytes by Fc fragments. I. The requirement for two signals in the generation of the polyclonal antibody response induced by Fc fragments. J Immunol. 1980 Mar;124(3):1330–1335. [PubMed] [Google Scholar]
  14. Morgan E. L., Weigle W. O. Regulation of Fc fragment-induced murine spleen cell proliferation. J Exp Med. 1980 Jan 1;151(1):1–11. doi: 10.1084/jem.151.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morgan E. L., Weigle W. O. The requirement for adherent cells in the Fc fragment-induced proliferative response of murine spleen cells. J Exp Med. 1979 Aug 1;150(2):256–266. doi: 10.1084/jem.150.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Okada M., Henney C. S. The differentiation of cytotoxic T cells in vitro. II. Amplifying factor(s) produced in primary mixed lymphocyte cultures against K/D stimuli require the presence of Lyt 2+ cells but not Lyt 1+ cells. J Immunol. 1980 Jul;125(1):300–307. [PubMed] [Google Scholar]
  17. Okada M., Klimpel G. R., Kuppers R. C., Henney C. S. The differentiation of cytotoxic T cells in vitro. I. Amplifying factor(s) in the primary response is Lyt 1 + cell dependent. J Immunol. 1979 Jun;122(6):2527–2533. [PubMed] [Google Scholar]
  18. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schrier R. D., Skidmore B. J., Kurnick J. T., Goldstine S. N., Chiller J. M. Propagation of antigen-specific T cell helper function in vitro. J Immunol. 1979 Dec;123(6):2525–2531. [PubMed] [Google Scholar]
  20. Shiku H., Takahashi T., Bean M. A., Old L. J., Oettgen H. F. Ly phenotype of cytotoxic T cells for syngeneic tumor. J Exp Med. 1976 Oct 1;144(4):1116–1120. doi: 10.1084/jem.144.4.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stutman O., Shen F. W., Boyse E. A. Ly phenotype of T cells cytotoxic for syngeneic mouse mammary tumors: evidence for T cell interactions. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5667–5671. doi: 10.1073/pnas.74.12.5667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Swain S. L., Bakke A., English M., Dutton R. W. Ly phenotypes and MHC recognition: the allohelper that recognizes K or D is a mature Ly123 cell. J Immunol. 1979 Dec;123(6):2716–2724. [PubMed] [Google Scholar]
  23. Swain S. L., Panfili P. R. Helper cells activated by allogeneic H-2K or H-2D differences have a Ly phenotype distinct from those responsive to I differences. J Immunol. 1979 Feb;122(2):383–391. [PubMed] [Google Scholar]
  24. Thoman M. L., Morgan E. L., Weigle W. O. Fc fragment activation of T lymphocytes. I. Fc fragments trigger Lyt 1+23- T lymphocytes to release a helper T cell-replacing activity. J Immunol. 1981 Feb;126(2):632–635. [PubMed] [Google Scholar]
  25. Thoman M. L., Morgan E. L., Weigle W. O. Polyclonal activation of murine B lymphocytes by Fe fragments. II. Replacement of T cells by a soluble helper T cell-replacing factor (TRF). J Immunol. 1980 Oct;125(4):1630–1633. [PubMed] [Google Scholar]
  26. Wagner H., Götze D., Ptschelinzew L., Röllinghoff M. Induction of cytotoxic T lymphocytes against I-region-coded determinants: in vitro evidence for a third histocompatibility locus in the mouse. J Exp Med. 1975 Dec 1;142(6):1477–1487. doi: 10.1084/jem.142.6.1477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wagner H., Röllinghoff M. T-T-cell interactions during the vitro cytotoxic allograft responses. I. Soluble products from activated Lyl+ T cells trigger autonomously antigen-primed Ly23+ T cells to cell proliferation and cytolytic activity. J Exp Med. 1978 Dec 1;148(6):1523–1538. doi: 10.1084/jem.148.6.1523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wettstein P. J., Bailey D. W., Mobraaten L. E., Klein J., Frelinger J. A. T-lymphocyte response to H-2 mutants. I. Proliferation is dependent on Ly 1+2+ cells. J Exp Med. 1978 May 1;147(5):1395–1404. doi: 10.1084/jem.147.5.1395. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES