Abstract
An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the kappa-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active kappa light chain gene.
Full Text
The Full Text of this article is available as a PDF (274.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barstad P., Hubert J., Hunkapiller M., Goetze A., Schilling J., Black B., Eaton B., Richards J., Weigert M., Hood L. Immunoglobulins with hapten-binding activity: structure-function correlations and genetic implications. Eur J Immunol. 1978 Jul;8(7):497–503. doi: 10.1002/eji.1830080709. [DOI] [PubMed] [Google Scholar]
- Barstad P., Rudikoff S., Potter M., Cohn M., Konigsberg W., Hood L. Immunoglobulin structure: amino terminal sequences of mouse myeloma proteins that bind phosphorylcholine. Science. 1974 Mar 8;183(4128):962–966. doi: 10.1126/science.183.4128.962. [DOI] [PubMed] [Google Scholar]
- Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
- Blattner F. R., Williams B. G., Blechl A. E., Denniston-Thompson K., Faber H. E., Furlong L., Grunwald D. J., Kiefer D. O., Moore D. D., Schumm J. W. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science. 1977 Apr 8;196(4286):161–169. doi: 10.1126/science.847462. [DOI] [PubMed] [Google Scholar]
- Claflin J. L., Davie J. M. Clonal nature of the immune response to phosphorylcholine. IV. Idiotypic uniformity of binding site-associated antigenic determinants among mouse antiphosphorylcholine antibodies. J Exp Med. 1974 Sep 1;140(3):673–686. doi: 10.1084/jem.140.3.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cook W. D., Scharff M. D. Antigen-binding mutants of mouse myeloma cells. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5687–5691. doi: 10.1073/pnas.74.12.5687. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Köhler H. The response to phosphorylcholine: dissecting an immune response. Transplant Rev. 1975;27:24–56. [PubMed] [Google Scholar]
- Max E. E., Seidman J. G., Leder P. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450–3454. doi: 10.1073/pnas.76.7.3450. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Polsky F., Edgell M. H., Seidman J. G., Leder P. High capacity gel preparative electrophoresis for purification of fragments of genomic DNA. Anal Biochem. 1978 Jul 1;87(2):397–410. doi: 10.1016/0003-2697(78)90689-9. [DOI] [PubMed] [Google Scholar]
- Rudikoff S., Potter M. Size differences among immunoglobulin heavy chains from phosphorylcholine-binding proteins. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2109–2112. doi: 10.1073/pnas.73.6.2109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rudikoff S., Potter M. kappa Chain variable region from M167, a phosphorylcholine binding myeloma protein. Biochemistry. 1978 Jul 11;17(14):2703–2707. doi: 10.1021/bi00607a001. [DOI] [PubMed] [Google Scholar]