Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1981 Jun 1;153(6):1415–1425. doi: 10.1084/jem.153.6.1415

Antigen- and receptor-driven regulatory mechanisms. VIII. Suppression of idiotype-negative, p-azobenzenearsonate-specific T cells results from the interaction of an anti-idiotypic second-order T suppressor cell with a cross-reactive-idiotype-positive, p-azobenzenearsonate- primed T cell target

PMCID: PMC2186180  PMID: 6454748

Abstract

The suppressor pathway that regulates the T cell response to p- azobenzenearsonate (ABA)-coupled cells has been studied. It has been found that the ability of anti-idiotypic second-order T suppressor cells (Ts2) to inhibit T cell-dependent delayed-type hypersensitivity (DTH) responses depended upon the presence of cross-reactive-idiotype (CRI)-bearing T cells present in ABA-primed mice. This suppressor T cell subset, termed Ts2, so exists with CRI-negative T cells that mediate DTH in vivo. It appears that antigen-activated CRI+ Ts3 require signals from the anti-CRI Ts2 subset to suppress DTH reactions in an idiotype-nonspecific manner. The relevance of these observations to a comprehensive scheme of T and B cell regulation is discussed.

Full Text

The Full Text of this article is available as a PDF (784.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach B. A., Sherman L., Benacerraf B., Greene M. I. Mechanisms of regulation of cell-mediated immunity. II. Induction and suppression of delayed-type hypersensitivity to azobenzenearsonate-coupled syngeneic cells. J Immunol. 1978 Oct;121(4):1460–1468. [PubMed] [Google Scholar]
  2. Benacerraf B., Germain R. N. A single major pathway of T-lymphocyte interactions in antigen-specific immune suppression. Scand J Immunol. 1981;13(1):1–10. doi: 10.1111/j.1365-3083.1981.tb00104.x. [DOI] [PubMed] [Google Scholar]
  3. Bromberg J. S., Benacerraf B., Greene M. I. Mechanisms of regulation of cell-mediated immunity. VII. Suppressor T cells induced by suboptimal doses of antigen plus an I-J-specific allogeneic effect. J Exp Med. 1981 Feb 1;153(2):437–449. doi: 10.1084/jem.153.2.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cantor H., Asofsky R. Synergy among lymphoid cells mediating the graft-versus-host response. 3. Evidence for interaction between two types of thymus-derived cells. J Exp Med. 1972 Apr 1;135(4):764–779. doi: 10.1084/jem.135.4.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Claman H. N. Hypothesis. T-cell tolerance--one signal? Cell Immunol. 1979 Nov;48(1):201–207. doi: 10.1016/0008-8749(79)90112-6. [DOI] [PubMed] [Google Scholar]
  6. Dietz M. H., Sy M. S., Benacerraf B., Nisonoff A., Greene M. I., Germain R. N. Antigen- and receptor-driven regulatory mechanisms. VII. H-2-restricted anti-idiotypic suppressor factor from efferent suppressor T cells. J Exp Med. 1981 Feb 1;153(2):450–463. doi: 10.1084/jem.153.2.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dietz M. H., Sy M. S., Greene M. I., Nisonoff A., Benacerraf B., Germain R. N. Antigen and receptor-driven regulatory mechanisms. VI. Demonstration of cross-reactive idiotypic determinants on azobenzenearsonate-specific antigen-binding suppressor T cells producing soluble suppressor factor(s). J Immunol. 1980 Dec;125(6):2374–2379. [PubMed] [Google Scholar]
  8. Feldmann M., Kilburn D. G., Levy J. T-T interaction in the generation of helper cells in vitro. Nature. 1975 Aug 28;256(5520):741–743. doi: 10.1038/256741a0. [DOI] [PubMed] [Google Scholar]
  9. Hirai Y., Nisonoff A. Selective suppression of the major idiotypic component of an antihapten response by soluble T cell-derived factors with idiotypic or anti-idiotypic receptors. J Exp Med. 1980 May 1;151(5):1213–1231. doi: 10.1084/jem.151.5.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Howe M. L., Cohen L. Lymphoid cell subpopulations. I. Synergy between lymph node cells and thymocytes in response to alloantigens and mitogens. J Immunol. 1975 Nov;115(5):1227–1232. [PubMed] [Google Scholar]
  11. Kuettner M. G., Wang A. L., Nisonoff A. Quantitative investigations of idiotypic antibodies. VI. Idiotypic specificity as a potential genetic marker for the variable regions of mouse immunoglobulin polypeptide chains. J Exp Med. 1972 Mar 1;135(3):579–595. doi: 10.1084/jem.135.3.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maguire H. C., Jr, Ettore V. L. Enhancement of dinitrochlorobenzene (DNCB) contact sensitization by cyclophosphamide in the guinea pig. J Invest Dermatol. 1967 Jan;48(1):39–43. doi: 10.1038/jid.1967.6. [DOI] [PubMed] [Google Scholar]
  13. Owen F. L., Ju S. T., Nisonoff A. Presence on idiotype-specific suppressor T cells of receptors that interact with molecules bearing the idiotype. J Exp Med. 1977 Jun 1;145(6):1559–1566. doi: 10.1084/jem.145.6.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Polak L., Turk J. L. Reversal of immunological tolerance by cyclophosphamide through inhibition of suppressor cell activity. Nature. 1974 Jun 14;249(458):654–656. doi: 10.1038/249654a0. [DOI] [PubMed] [Google Scholar]
  15. Sy M. S., Bach B. A., Brown A., Nisonoff A., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. II. Induction of suppressor T cells with idiotype-coupled syngeneic spleen cells. J Exp Med. 1979 Nov 1;150(5):1229–1240. doi: 10.1084/jem.150.5.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sy M. S., Brown A. R., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. III. Induction of delayed type hypersensitivity to azobenzenearsonate with anti-cross-reactive idiotypic antibodies. J Exp Med. 1980 Apr 1;151(4):896–909. doi: 10.1084/jem.151.4.896. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sy M. S., Dietz M. H., Germain R. N., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. IV. Idiotype-bearing I-J+ suppressor T cell factors induce second-order suppressor T cells which express anti-idiotypic receptors. J Exp Med. 1980 May 1;151(5):1183–1195. doi: 10.1084/jem.151.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sy M. S., Dietz M. H., Nisonoff A., Germain R. N., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. V. The failure of idiotype-coupled spleen cells to induce unresponsiveness in animals lacking the appropriate VH genes is caused by the lack of idiotype-matched targets. J Exp Med. 1980 Nov 1;152(5):1226–1235. doi: 10.1084/jem.152.5.1226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sy M. S., Miller S. D., Claman H. N. Immune suppression with supraoptimal doses of antigen in contact sensitivity. I. Demonstration of suppressor cells and their sensitivity to cyclophosphamide. J Immunol. 1977 Jul;119(1):240–244. [PubMed] [Google Scholar]
  20. Sy M. S., Miller S. D., Moorhead J. W., Claman H. N. Active suppression of 1-fluoro-2,4-dinitrobenzene-immune T cells. Requirement of an auxiliary T cell induced by antigen. J Exp Med. 1979 May 1;149(5):1197–1207. doi: 10.1084/jem.149.5.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Teh H. S., Teh S. J. Direct evidence for a two-signal mechanism of cytotoxic T-lymphocyte activation. Nature. 1980 May 15;285(5761):163–165. doi: 10.1038/285163a0. [DOI] [PubMed] [Google Scholar]
  22. Wagner H. Synergy during in vitro cytotoxic allograft responses. I. Evidence for cell interaction between thymocytes and peripheral T cells. J Exp Med. 1973 Dec 1;138(6):1379–1397. doi: 10.1084/jem.138.6.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weinberger J. Z., Germain R. N., Benacerraf B., Dorf M. E. Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. V. Role of idiotypes in the suppressor pathway. J Exp Med. 1980 Jul 1;152(1):161–169. doi: 10.1084/jem.152.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wetzig R., Hanson D. G., Miller S. D., Claman H. N. Binding of ovalbumin to mouse spleen cells with and without carbodiimide. J Immunol Methods. 1979;28(3-4):361–368. doi: 10.1016/0022-1759(79)90201-1. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES