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. 1981 Jun 1;153(6):1672–1677. doi: 10.1084/jem.153.6.1672

Presence of interchain disulfide bonds between two gene products that compose the secreted form of an antigen-specific suppressor factor

PMCID: PMC2186189  PMID: 6166720

Abstract

The secreted form of the suppressor T cell factor specific for keyhole limpet hemocyanin derived from the hybridoma 34S-704 was found to consist of the two distinct polypeptide chains, i.e., the antigen- binding and the I-J-encoded chains. They were linked in covalent association with disulfide bonds. The two chains were cleaved by the reduction with dithiothreitol and were easy to reconstitute the active form of TsF. The association of the two distinct chains was suggested to be essential for the expression of the TsF activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Araneo B. A., Kapp J. A. Immunosuppressive factor(s) from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT). V. Inhibition of T cell proliferative responses. J Immunol. 1980 Jul;125(1):118–123. [PubMed] [Google Scholar]
  2. Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
  3. Bach B. A., Greene M. I., Benacerraf B., Nisonoff A. Mechanisms of regulation of cell-mediated immunity. IV. Azobenzenearsonate-specific suppressor factor(s) bear cross-reactive idiotypic determinants the expression of which is linked to the heavy-chain allotype linkage group of genes. J Exp Med. 1979 May 1;149(5):1084–1098. doi: 10.1084/jem.149.5.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gall W. E., Cunningham B. A., Waxdal M. J., Konigsberg W. H., Edelman G. M. The covalent structure of a human gamma G-immunoglobulin. IV. The interchain disulfide bonds. Biochemistry. 1968 May;7(5):1973–1982. doi: 10.1021/bi00845a048. [DOI] [PubMed] [Google Scholar]
  5. Germain R. N., Ju S. T., Kipps T. J., Benacerraf B., Dorf M. E. Shared idiotypic determinants on antibodies and T-cell-derived suppressor factor specific for the random terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10. J Exp Med. 1979 Mar 1;149(3):613–622. doi: 10.1084/jem.149.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kontiainen S., Simpson E., Bohrer E., Beverley P. C., Herzenberg L. A., Fitzpatrick W. C., Vogt P., Torano A., McKenzie I. F., Feldmann M. T-cell lines producing antigen-specific suppressor factor. Nature. 1978 Aug 3;274(5670):477–480. doi: 10.1038/274477a0. [DOI] [PubMed] [Google Scholar]
  7. Sy M. S., Dietz M. H., Germain R. N., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. IV. Idiotype-bearing I-J+ suppressor T cell factors induce second-order suppressor T cells which express anti-idiotypic receptors. J Exp Med. 1980 May 1;151(5):1183–1195. doi: 10.1084/jem.151.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tada T., Hayakawa K., Okumura K., Taniguchi M. Coexistence of variable region of immunoglobulin heavy chain and I region gene products on antigen-specific suppressor T cells and suppressor T cell factor. A minimal model of functional antigen receptor of T cells. Mol Immunol. 1980 Jul;17(7):867–875. doi: 10.1016/0161-5890(80)90035-8. [DOI] [PubMed] [Google Scholar]
  9. Tada T., Taniguchi M., David C. S. Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. J Exp Med. 1976 Sep 1;144(3):713–725. doi: 10.1084/jem.144.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Taniguchi M., Miller J. F. Enrichment of specific suppressor T cells and characterization of their surface markers. J Exp Med. 1977 Nov 1;146(5):1450–1454. doi: 10.1084/jem.146.5.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Taniguchi M., Saito T., Tada T. Antigen-specific suppressive factor produced by a transplantable I-J bearing T-cell hybridoma. Nature. 1979 Apr 5;278(5704):555–558. doi: 10.1038/278555a0. [DOI] [PubMed] [Google Scholar]
  12. Taniguchi M., Takei I., Tada T. Functional and molecular organisation of an antigen-specific suppressor factor from a T-cell hybridoma. Nature. 1980 Jan 10;283(5743):227–228. doi: 10.1038/283227a0. [DOI] [PubMed] [Google Scholar]
  13. Taussig M. J., Munro A. J., Campbell R., David C. S., Staines N. A. Antigen-specific T-cell factor in cell cooperation. Mapping within the I region of the H-2 complex and ability to cooperate across allogeneic barriers. J Exp Med. 1975 Sep 1;142(3):694–700. doi: 10.1084/jem.142.3.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thèze J., Waltenbaugh C., Germain R. N., Benacerraf B. Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyosine50. IV. In vitro activity and immunochemical properties. Eur J Immunol. 1977 Oct;7(10):705–710. doi: 10.1002/eji.1830071011. [DOI] [PubMed] [Google Scholar]
  15. Tokuhisa T., Taniguchi M., Okumura K., Tada T. An antigen-specific I region gene product that augments the antibody response. J Immunol. 1978 Feb;120(2):414–421. [PubMed] [Google Scholar]

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