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. 1981 Jun 1;153(6):1592–1603. doi: 10.1084/jem.153.6.1592

Interaction of target cell-bound C3bi and C3d with human lymphocyte receptors. Enhancement of antibody-mediated cellular cytotoxicity

PMCID: PMC2186201  PMID: 7252421

Abstract

The occurrence and distribution of distinct receptors for three C3 fragments on purified human blood lymphocytes were studied by rosette formation. Indicator cells were bovine, chicken, or sheep erythrocytes (E) bearing up to 100,000 molecules of human C3b (EC3b) without antibody. EC3b was converted to C3bi-bearing-E (EC3bi) with purified C3b inactivator (factor I) and beta1H (factor H), and to C3d-bearing E (EC3d) by treatment of EC3bi with trypsin. Using bovine E (Eb) as indicators, approximately 11% of the lymphocytes bound EbC3b, 6% bound EbC3bi and 2% bound EbC3d. Fractionation of the lymphocytes by adsorption to monolayers of C3-fragment-bearing Eb or by rosetting indicated that most of the cells with receptors for C3b were distinct from those having receptors for C3bi and/or C3d. Cells from two lymphoblastoid cell lines (Raji and Daudi) formed strong rosettes with EC3b, which were weak. 51Cr-labeled E was used as a target in antibody, C3-fragment-bearing E was not lysed by the lymphocytes. However, at suboptimal concentrations of IgG enhancing capacity of the fragments occurred in the order of C3bi greater than C3d greater than C3b. In addition, C3-fragment-bearing cells inhibited the lysis of antibody- coated cells not concluded that target cell bound C3 fragments enhance ADCC by improving contact between target cells and those effector cells which have C3 receptors. Cell-bound C3 effector cells. It is proposed that certain lymphocytes are capable of interacting with C3bi in addition to C3b and C3d and that C3bi and C3d have a greater regulatory effect on their cytolytic function than C3b.

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Selected References

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  1. Carlo J. R., Ruddy S., Studer E. J., Conrad D. H. Complement receptor binding of C3b-coated cells treated with C3b inactivator, beta 1H globulin and trypsin. J Immunol. 1979 Aug;123(2):523–528. [PubMed] [Google Scholar]
  2. Cooper N. R. Immune adherence by the fourth component of complement. Science. 1969 Jul 25;165(3891):396–398. doi: 10.1126/science.165.3891.396. [DOI] [PubMed] [Google Scholar]
  3. Cordier G., Samarut C., Revillard J. P. Changes of Fcgamma receptor-related properties induced by interaction of human lymphocytes with insoluble immune complexes. J Immunol. 1977 Dec;119(6):1943–1948. [PubMed] [Google Scholar]
  4. Dierich M. P., Bokisch V. A. Receptor-binding sites on C3 and C3b. J Immunol. 1977 Jun;118(6):2145–2150. [PubMed] [Google Scholar]
  5. Dierich M. P., Pellegrino M. A., Ferrone S., Reisfeld R. A. Evaluation of C3 receptors on lymphoid cells with different complement sources. J Immunol. 1974 May;112(5):1766–1773. [PubMed] [Google Scholar]
  6. Ghebrehiwet B., Medicus R. G., Müller-Eberhard H. J. Potentiation of antiboty-dependent cell-mediated cytotoxicity by target cell-bound C3b. J Immunol. 1979 Sep;123(3):1285–1288. [PubMed] [Google Scholar]
  7. Härfast B., Andersson T., Alsheikhly A., Perlmann P. Effect of Fc-receptor modulation on mumps-virus-dependent lymphocyte-mediated cytotoxicity in vitro. Scand J Immunol. 1980;11(3):357–362. doi: 10.1111/j.1365-3083.1980.tb00246.x. [DOI] [PubMed] [Google Scholar]
  8. Lustig H. J., Bianco C. Antibody-mediated cell cytotoxicity in a defined system: regulation by antigen, antibody, and complement. J Immunol. 1976 Jan;116(1):253–260. [PubMed] [Google Scholar]
  9. Moretta L., Mingari M. C., Romanzi C. A. Loss of Fc receptors for IgG from human T lymphocytes exposed to IgG immune complexes. Nature. 1978 Apr 13;272(5654):618–620. doi: 10.1038/272618a0. [DOI] [PubMed] [Google Scholar]
  10. Müller-Eberhard H. J., Schreiber R. D. Molecular biology and chemistry of the alternative pathway of complement. Adv Immunol. 1980;29:1–53. doi: 10.1016/s0065-2776(08)60042-5. [DOI] [PubMed] [Google Scholar]
  11. O'Neill P., Mackler B. F., Wyde P. Complement (C3) receptor-bearing lymphocyte-mediated cytotoxicity and lymphotoxin responses. Cell Immunol. 1975 Nov;20(1):33–41. doi: 10.1016/0008-8749(75)90081-7. [DOI] [PubMed] [Google Scholar]
  12. Pangburn M. K., Müller-Eberhard H. J. Complement C3 convertase: cell surface restriction of beta1H control and generation of restriction on neuraminidase-treated cells. Proc Natl Acad Sci U S A. 1978 May;75(5):2416–2420. doi: 10.1073/pnas.75.5.2416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pangburn M. K., Schreiber R. D., Müller-Eberhard H. J. Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution. J Exp Med. 1977 Jul 1;146(1):257–270. doi: 10.1084/jem.146.1.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pape G. R., Moretta L., Troye M., Perlmann P. Natural cytotoxicity of human Fc gamma-receptor-positive T lymphocytes after surface modulation with immune complexes. Scand J Immunol. 1979 Mar;9(3):291–296. doi: 10.1111/j.1365-3083.1979.tb02734.x. [DOI] [PubMed] [Google Scholar]
  15. Perlmann H., Perlmann P., Pape G. R., Halldén G. Purification, fractionation and assay of antibody-dependent lymphocytic effector cells (K cells) in human blood. Scand J Immunol. 1976 Jun;Suppl 5:57–68. doi: 10.1111/j.1365-3083.1976.tb03856.x. [DOI] [PubMed] [Google Scholar]
  16. Perlmann P., Perlmann H., Müller-Eberhard H. J. Cytolytic lymphocytic cells with complement receptor in human blood. Induction of cytolysis by IgG antibody but not by target cell-bound C3. J Exp Med. 1975 Feb 1;141(2):287–296. doi: 10.1084/jem.141.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perlmann P., Perlmann H., Wigzell H. Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells. Transplant Rev. 1972;13:91–114. doi: 10.1111/j.1600-065x.1972.tb00061.x. [DOI] [PubMed] [Google Scholar]
  18. Ross G. D., Polley M. J., Rabellino E. M., Grey H. M. Two different complement receptors on human lymphocytes. One specific for C3b and one specific for C3b inactivator-cleaved C3b. J Exp Med. 1973 Oct 1;138(4):798–811. doi: 10.1084/jem.138.4.798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schreiber R. D., Medicus R. G., Gïtze O., Müller-Eberhard H. J. Properdin- and nephritic factor-dependent C3 convertases: requirement of native C3 for enzyme formation and the function of bound C3b as properdin receptor. J Exp Med. 1975 Sep 1;142(3):760–772. doi: 10.1084/jem.142.3.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schreiber R. D., Pangburn M. K., Lesavre P. H., Müller-Eberhard H. J. Initiation of the alternative pathway of complement: recognition of activators by bound C3b and assembly of the entire pathway from six isolated proteins. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3948–3952. doi: 10.1073/pnas.75.8.3948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Soulillou J. P., Carpenter C. B., d'Apice A. J., Strom T. B. The role of nonclassical Fc receptor-associated, Ag-B antigens (Ia) in rat allograft enhancement. J Exp Med. 1976 Feb 1;143(2):405–421. doi: 10.1084/jem.143.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tack B. D., Prahl J. W. Third component of human complement: purification from plasma and physicochemical characterization. Biochemistry. 1976 Oct 5;15(20):4513–4521. doi: 10.1021/bi00665a028. [DOI] [PubMed] [Google Scholar]
  23. Theofilopoulos A. N., Dixon F. J., Bokisch V. A. Binding of soluble immune complexes to human lymphoblastoid cells. I. Characterization of receptors for IgG Fc and complement and description of the binding mechanism. J Exp Med. 1974 Oct 1;140(4):877–894. doi: 10.1084/jem.140.4.877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Van Boxel J. A., Paul W. E., Green I., Frank M. M. Antibody-dependent lymphoid cell-mediated cytotoxicity: role of complement. J Immunol. 1974 Jan;112(1):398–403. [PubMed] [Google Scholar]
  25. Wåhlin B., Perlmann H., Perlmann P. Analysis by a plaque assay of IgG- or IgM- dependent cytolytic lymphocytes in human blood. J Exp Med. 1976 Nov 2;144(5):1375–1380. doi: 10.1084/jem.144.5.1375. [DOI] [PMC free article] [PubMed] [Google Scholar]

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