Abstract
The mannan of bakers' yeast (Saccharomyces cerevisiae) was fractionated on a column of diethylaminoethyl-Sephadex into five subfractions. Phosphate content of these mannan subfractions was proportional to the concentration of NaCl solutions used in the chromatographic separation. Quantitative precipitin reactions showed that the serological reactivities of the subfractions were proportional to the content of phosphate. The result of acetolysis study showed that the amounts of mannotetraose and phosphate-containing oligosaccharide fractions increased proportionally to the acidity, whereas the amount of mannose decreased inversely. The results from quantitative precipitin reaction tests and acetolysis study demonstrated that both phosphate contents and multiplicity of branching moieties of mannan subfractions increased proportionally, i.e., micro-heterogeneity concerning the acidity comprised in the parent bulk mannan is not attributable merely to the coexistence of molecular species containing different amounts of phosphate but also to the presence of more of the branching moieties.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
- Colonna W. J., Lampen J. O. Structure of the mannan form Saccharomyces strain FH4C, a mutant constitutive for invertase biosynthesis. I. Significance of phosphate to the structure and refractoriness of the molecule. Biochemistry. 1974 Jun 18;13(13):2741–2748. doi: 10.1021/bi00710a013. [DOI] [PubMed] [Google Scholar]
- Kocourek J., Ballou C. E. Method for fingerprinting yeast cell wall mannans. J Bacteriol. 1969 Dec;100(3):1175–1181. doi: 10.1128/jb.100.3.1175-1181.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kozak L. P., Bretthauer R. K. Studies on the biosynthesis of Hansenula holstii mannans from guanosine diphosphate mannose. Biochemistry. 1970 Mar 3;9(5):1115–1122. doi: 10.1021/bi00807a010. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lloyd K. O. Isolation, characterization, and partial structure of peptido galactomannans from the yeast form of Cladosporium werneckii. Biochemistry. 1970 Aug 18;9(17):3446–3453. doi: 10.1021/bi00819a025. [DOI] [PubMed] [Google Scholar]
- Nakajima T., Ballou C. E. Characterization of the carbohydrate fragments obtained from Saccharomyces cerevisiae mannan by alkaline degradation. J Biol Chem. 1974 Dec 10;249(23):7679–7684. [PubMed] [Google Scholar]
- Raschke W. C., Ballou C. E. Immunochemistry of the phosphomannan of the yeast Kloeckera brevis. Biochemistry. 1971 Oct 26;10(22):4130–4135. doi: 10.1021/bi00798a018. [DOI] [PubMed] [Google Scholar]
- Stewart T. S., Ballou C. E. A comparison of yeast mannans and phosphomannans by acetolysis. Biochemistry. 1968 May;7(5):1855–1863. doi: 10.1021/bi00845a033. [DOI] [PubMed] [Google Scholar]
- Sunayama H. Studies on the antigenic activities of yeasts. IV. Analysis of the antigenic determinant groups of the mannan of Candida albicans serotype A. Jpn J Microbiol. 1970 Jan;14(1):27–39. doi: 10.1111/j.1348-0421.1970.tb00488.x. [DOI] [PubMed] [Google Scholar]
- Suzuki S., Sunayama H., Saito T. Studies on the antigenic activity of yeasts. I. Analysis of the determinant groups of the mannan of Saccharomyces cerevisiae. Jpn J Microbiol. 1968 Mar;12(1):19–24. doi: 10.1111/j.1348-0421.1968.tb00365.x. [DOI] [PubMed] [Google Scholar]
- Thieme T. R., Ballou C. E. Nature of the phosphodiester linkage of the phosphomannan from the yeast Kloeckera brevis. Biochemistry. 1971 Oct 26;10(22):4121–4129. doi: 10.1021/bi00798a017. [DOI] [PubMed] [Google Scholar]
- Vaskovsky V. E., Svetashev V. I. Phospholipid spray reagents. J Chromatogr. 1972 Feb 23;65(2):451–453. doi: 10.1016/s0021-9673(00)92571-8. [DOI] [PubMed] [Google Scholar]