Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Oct;136(1):104–110. doi: 10.1128/jb.136.1.104-110.1978

Role of small molecules in regulation of D-serine deaminase synthesis.

M C Heincz, E McFall
PMCID: PMC218637  PMID: 213417

Abstract

Cyclic AMP is required for optimal synthesis of D-serine deaminase synthesis from dsdO+ templates and for optimal hyperinducible synthesis from low constitutive dsdO templates both in vitro and in vivo. Neither D-serine, cyclic AMP, nor dsdC activator has an effect on expression of a high constitutive dsdO template. The synthesis of the dsdC activator itself in vitro is independent of cyclic AMP. Guanosine tetraphosphate does not have a significant effect on in vitro D-serine deaminase synthesis from dsdO+ or dsdO templates. A previously described class of dsdO mutants showing partial catabolite sensitivity of constitutive D-serine deaminase synthesis proved to be low dsdO types. They all contain a low constitutive dsdC mutation; the two effects are additive with regard to level of constitutivity, but only that portion of synthesis attributable to the dsdC mutation is cyclic AMP dependent.

Full text

PDF
104

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloom F. R. Isolation and characterization of catabolite-resistant mutants in the D-serine deaminase system of Escherichia coli K-12. J Bacteriol. 1975 Mar;121(3):1085–1091. doi: 10.1128/jb.121.3.1085-1091.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bloom F. R., McFall E. Isolation and characterization of D-serine deaminase constitutive mutants by utilization of D-serine as sole carbon or nitrogen source. J Bacteriol. 1975 Mar;121(3):1078–1084. doi: 10.1128/jb.121.3.1078-1084.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bloom F. R., McFall E., Young M. C., Carothers A. M. Positive control in the D-serine deaminase system of Escherichia coli K-12. J Bacteriol. 1975 Mar;121(3):1092–1101. doi: 10.1128/jb.121.3.1092-1101.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chamberlin M. J. The selectivity of transcription. Annu Rev Biochem. 1974;43(0):721–775. doi: 10.1146/annurev.bi.43.070174.003445. [DOI] [PubMed] [Google Scholar]
  5. Colomé J., Wilcox G., Englesberg E. Constitutive mutations in the controlling site region of the araBAD operon of Escherichia coli B/r that decrease sensitivity to catabolite repression. J Bacteriol. 1977 Feb;129(2):948–958. doi: 10.1128/jb.129.2.948-958.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heincz M. C., Kelker N. E., McFall E. Positive control of D-serine deaminase synthesis in vitro. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1695–1699. doi: 10.1073/pnas.75.4.1695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heincz M. C., McFall E. Role of the dsdC activator in regulation of D-serine deaminase synthesis. J Bacteriol. 1978 Oct;136(1):96–103. doi: 10.1128/jb.136.1.96-103.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Maizels N. M. The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585–3589. doi: 10.1073/pnas.70.12.3585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McFall E. Mapping of the d-serine deaminase region in Escherichia coli K-12. Genetics. 1967 Jan;55(1):91–99. doi: 10.1093/genetics/55.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McFall E. Role of adenosine 3',5'-cyclic monophosphate and its specific binding protein in the regulation of D-serine deaminase synthesis. J Bacteriol. 1973 Feb;113(2):781–785. doi: 10.1128/jb.113.2.781-785.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Palchaudhuri S., McFall E., Carothers A. M. Specialized transduction of D-serine deaminase genes: formation of lysogens that yield high lambda-d dsd/lambda ratios and formation of a dimeric lambda-d dsd. J Bacteriol. 1976 Aug;127(2):998–1014. doi: 10.1128/jb.127.2.998-1014.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tyler B., Deleo A. B., Magasanik B. Activation of transcription of hut DNA by glutamine synthetase. Proc Natl Acad Sci U S A. 1974 Jan;71(1):225–229. doi: 10.1073/pnas.71.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yang H. L., Zubay G., Urm E., Heiness G., Cashel M. Effects of guanosine tetraphosphate, guanosine pentaphosphate, and beta-gamma methylenyl-guanosine pentaphosphate on gene expression of Escherichia coli in vitro. Proc Natl Acad Sci U S A. 1974 Jan;71(1):63–67. doi: 10.1073/pnas.71.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yang H., Zubay G. A possible termination factor for transcription in Escherichia coli. Biochem Biophys Res Commun. 1974 Feb 4;56(3):725–731. doi: 10.1016/0006-291x(74)90665-2. [DOI] [PubMed] [Google Scholar]
  16. Zubay G. In vitro synthesis of protein in microbial systems. Annu Rev Genet. 1973;7:267–287. doi: 10.1146/annurev.ge.07.120173.001411. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES