Abstract
Human cell lines that resemble precursors in the B cell lineage have been found to synthesize J chain. In vivo pulse labeling, together with in vitro translation of total cellular RNA in a wheat germ cell-free system, detected the synthesis of J chain in immunoglobulin-secreting cell lines, in a cell line with only surface IgM, as well as in the pre- B-like cell line Josh 4 and the round cell lines Josh 7 and KLM 2. The primary translation products of J chain from all of these cell lines were found to be indistinguishable from one another by serologic criteria, by relative mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by charge as judged by alkaline-urea gel electrophoresis, and by peptide mapping. These findings suggest that the onset of J chain biosynthesis represents a relatively early event in B cell ontogeny, occurring before the development of immunoglobulin polymer-secreting cells. Its role may, consequently, be fundamental to the biosynthesis of all immunoglobulins, at different stages of B cell differentiation.
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- Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
- Brandtzaeg P. Presence of J chain in human immunocytes containing various immunoglobulin classes. Nature. 1974 Nov 29;252(5482):418–420. doi: 10.1038/252418a0. [DOI] [PubMed] [Google Scholar]
- Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
- Dobberstein B., Blobel G. Functional interaction of plant ribosomes with animal microsomal membranes. Biochem Biophys Res Commun. 1977 Feb 21;74(4):1675–1682. doi: 10.1016/0006-291x(77)90637-4. [DOI] [PubMed] [Google Scholar]
- Erickson A. H., Blobel G. Early events in the biosynthesis of the lysosomal enzyme cathepsin D. J Biol Chem. 1979 Dec 10;254(23):11771–11774. [PubMed] [Google Scholar]
- Eskeland T. IgM reassociation in Vitro: no influence of J chain on the amount of polymers. Scand J Immunol. 1974;3(6):757–768. doi: 10.1111/j.1365-3083.1974.tb01311.x. [DOI] [PubMed] [Google Scholar]
- Fu S. M., Hurley J. N. Human cell lines containing Epstein-Barr virus but distinct from the common B cell lymphoblastoid lines. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6637–6640. doi: 10.1073/pnas.76.12.6637. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fu S. M., Hurley J. N., McCune J. M., Kunkel H. G., Good R. A. Pre-B cells and other possible precursor lymphoid cell lines derived from patients with X-linked agammaglobulinemia. J Exp Med. 1980 Dec 1;152(6):1519–1526. doi: 10.1084/jem.152.6.1519. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Inman F. P., Mestecky J. The J chain of polymeric immunoglobulins. Contemp Top Mol Immunol. 1974;3:111–141. doi: 10.1007/978-1-4684-2838-4_5. [DOI] [PubMed] [Google Scholar]
- Kaji H., Parkhouse M. E. Control of J chain biosynthesis in relation to heavy and light chain synthesis, polymerization and secretion. J Immunol. 1975 Apr;114(4):1218–1220. [PubMed] [Google Scholar]
- Koshland M. E. Structure and function of the J chain. Adv Immunol. 1975;20:41–69. doi: 10.1016/s0065-2776(08)60206-0. [DOI] [PubMed] [Google Scholar]
- Kownatzki E. Reassociation of IgM subunits in the presence and absence of J chain. Immunol Commun. 1973;2(1):105–113. doi: 10.3109/08820137309022886. [DOI] [PubMed] [Google Scholar]
- Mather E. L., Alt F. W., Bothwell A. L., Baltimore D., Koshland M. E. Expression of J chain RNA in cell lines representing different stages of B lymphocyte differentiation. Cell. 1981 Feb;23(2):369–378. doi: 10.1016/0092-8674(81)90132-x. [DOI] [PubMed] [Google Scholar]
- Mestecky J., Preud'homme J. L., Crago S. S., Mihaesco E., Prchal J. T., Okos A. J. Presence of J chain in human lymphoid cells. Clin Exp Immunol. 1980 Feb;39(2):371–385. [PMC free article] [PubMed] [Google Scholar]
- Mestecky J., Winchester R. J., Hoffman T., Kunkel H. G. Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen-stimulated normal cells and in lymphoblastoid cell lines. J Exp Med. 1977 Mar 1;145(3):760–765. doi: 10.1084/jem.145.3.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mihaesco C., Mihaesco E., Metzger H. Variable J-chain content in human IgM. FEBS Lett. 1973 Dec 1;37(2):303–306. doi: 10.1016/0014-5793(73)80483-1. [DOI] [PubMed] [Google Scholar]
- Mosmann T. R., Gravel Y., Williamson A. R., Baumal R. Modification and fate of J chain in myeloma cells in the presence and absence of polymeric immunoglobulin secretion. Eur J Immunol. 1978 Feb;8(2):94–101. doi: 10.1002/eji.1830080205. [DOI] [PubMed] [Google Scholar]
- Mosmann T., Baumal R. Synthesis but not secretion of J chain by variant mouse myeloma cells which lose alpha-chain-synthesizing ability. J Immunol. 1975 Oct;115(4):955–962. [PubMed] [Google Scholar]
- Nagura H., Brandtzaeg P., Nakane P. K., Brown W. R. Ultrastructural localization of J chain in human intestinal mucosa. J Immunol. 1979 Sep;123(3):1044–1050. [PubMed] [Google Scholar]
- Stott D. I. Biosynthesis and assembly of IgM. Addition of J chain to intracellular pools of 8S and 19S IgM. Immunochemistry. 1976 Feb;13(2):157–163. doi: 10.1016/0019-2791(76)90284-6. [DOI] [PubMed] [Google Scholar]
- Tomasi T. B., Czerwinski D. S. Naturally occurring polymers of IgA lacking J chain. Scand J Immunol. 1976;5(6-7):647–653. doi: 10.1111/j.1365-3083.1976.tb03014.x. [DOI] [PubMed] [Google Scholar]
- Wilde C. E., 3rd, Koshland M. E. Molecular size and shape of the J chain from polymeric immunoglobulins. Biochemistry. 1973 Aug 14;12(17):3218–3224. doi: 10.1021/bi00741a012. [DOI] [PubMed] [Google Scholar]