Abstract
T cells from strains responder to the antigen poly(Glu40 Ala60) (GA) were depleted of alloreactive cells by bromo-deoxyuridine and light treatment, and were subsequently primed in vitro in GA presented by allogeneic macrophages from nonresponder strains. Antigen-specific secondary proliferative responses restricted by allogeneic Ia molecules of the macrophages were obtained in all strain combinations tested. These data indicate that Ir gene-controlled nonresponsiveness cannot be the result of a failure of antigen presentation.
Full Text
The Full Text of this article is available as a PDF (285.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baxevanis C. N., Wernet D., Nagy Z. A., Maurer P. H., Klein J. Genetic control of T-cell proliferative responses to poly(glu40ala60) and poly(glu51lys34tyr15): subregion-specific inhibition of the responses with monoclonal Ia antibodies. Immunogenetics. 1980;11(6):617–628. doi: 10.1007/BF01567830. [DOI] [PubMed] [Google Scholar]
- Benacerraf B. A hypothesis to relate the specificity of T lymphocytes and the activity of I region-specific Ir genes in macrophages and B lymphocytes. J Immunol. 1978 Jun;120(6):1809–1812. [PubMed] [Google Scholar]
- Erb P., Vogt P., Matsunaga T., Rosenthal A., Feldmann M. Nature of T cell-macrophage interaction in helper cell induction in vitro. III. Responsiveness of T cells differentiating in irradiation or allophenic chimeras depends on the genotype of the host. J Immunol. 1980 Jun;124(6):2656–2664. [PubMed] [Google Scholar]
- Hedrick S. M., Watson J. Genetic control of the immune response to collagen. II. Antibody responses produced in fetal liver restored radiation chimeras and thymus reconstituted F1 hybrid nude mice. J Exp Med. 1979 Sep 19;150(3):646–652. doi: 10.1084/jem.150.3.646. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hodes R. J., Hathcock K. S., Singer A. Cellular and genetic control of antibody responses. VI. Expression of Ir gene function by H-2a accessory cells, but not H-2a T or B cells in responses to TNP-(T,G)-A--L. J Immunol. 1979 Dec;123(6):2823–2829. [PubMed] [Google Scholar]
- Janeway C. A., Jr, Murphy P. D., Kemp J., Wigzell H. T cells specific for hapten-modified self are precommitted for self major histocompatibility complex antigens before encounter with the hapten. J Exp Med. 1978 Apr 1;147(4):1065–1077. doi: 10.1084/jem.147.4.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kappler J. W., Marrack P. The role of H-2 linked genes in helper T-cell function. IV. Importance of T-cell genotype and host environment in I-region and Ir gene expression. J Exp Med. 1978 Dec 1;148(6):1510–1522. doi: 10.1084/jem.148.6.1510. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lemke H., Hämmerling G. J., Hämmerling U. Fine specificity analysis with monoclonal antibodies of antigens controlled by the major histocompatibility complex and by the Qa/TL region in mice. Immunol Rev. 1979;47:175–206. doi: 10.1111/j.1600-065x.1979.tb00293.x. [DOI] [PubMed] [Google Scholar]
- Longo D. L., Schwartz R. H. Gene complementation. Neither Ir-GLphi gene need be present in the proliferative T cell to generate an immune response to Poly(Glu55Lys36Phe9)n. J Exp Med. 1980 Jun 1;151(6):1452–1467. doi: 10.1084/jem.151.6.1452. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsunaga T., Simpson E. H-2 complementation in anti-H-Y cytotoxic T-cell responses can occur in chimeric mice. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6207–6210. doi: 10.1073/pnas.75.12.6207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller J. F., Gamble J., Mottram P., Smith F. I. Influence of thymus genotype on acquisition of responsiveness in delayed-type hypersensitivity. Scand J Immunol. 1979;9(1):29–38. doi: 10.1111/j.1365-3083.1979.tb02703.x. [DOI] [PubMed] [Google Scholar]
- Thomas D. W., Shevach E. M. Nature of the antigenic complex recognized by T lymphocytes: specific sensitization by antigens associated with allogeneic macrophages. Proc Natl Acad Sci U S A. 1977 May;74(5):2104–2108. doi: 10.1073/pnas.74.5.2104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson D. B., Lindahl K. F., Wilson D. H., Sprent J. The generation of killer cells to trinitrophenyl-modified allogeneic targets by lymphocyte populations negatively selected to strong alloantigens. J Exp Med. 1977 Aug 1;146(2):361–367. doi: 10.1084/jem.146.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- von Boehmer H., Haas W., Jerne N. K. Major histocompatibility complex-linked immune-responsiveness is acquired by lymphocytes of low-responder mice differentiating in thymus of high-responder mice. Proc Natl Acad Sci U S A. 1978 May;75(5):2439–2442. doi: 10.1073/pnas.75.5.2439. [DOI] [PMC free article] [PubMed] [Google Scholar]