Abstract
The common acute lymphoblastic leukemia antigen (CALLA), as defined by J-5 murine monoclonal antibodies, was detected on renal tubular and glomerular cells from fetal and adult donors by an indirect immunoperoxidase technique. CALLA could also be detected on epithelial cells of the fetal small intestine and on myoepithelial cells of adult breast but not on myoepithelial cells of the salivary gland. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of immunoprecipitated 125I-labeled membrane antigens from dissociated renal cells demonstrated that the antigen migrated as a 90,000 mol wt antigen rather than the 98,000-100,000 mol wt antigen noted on CALLA- positive tissue culture cell lines. The data suggest that the determinant defined by the J-5 monoclonal antibody is neither a lymphoid cell-specific differentiation antigen nor a leukemia-specific antigen.
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- Anderson J. K., Metzgar R. S. Detection and partial characterization of human T and B lymphocyte membrane antigens with antisera to HSB and SB cell lines. J Immunol. 1978 Jan;120(1):262–271. [PubMed] [Google Scholar]
- Billing R., Minowada J., Cline M., Clark B., Lee K. Acute lymphocytic leukemia-associated cell membrane antigen. J Natl Cancer Inst. 1978 Aug;61(2):423–429. [PubMed] [Google Scholar]
- Borella L., Sen L., Casper J. T. Acute lymphoblastic leukemia (ALL) antigens detected with antisera to E rosette-froming and non-E rosette-forming ALL blasts. J Immunol. 1977 Jan;118(1):309–315. [PubMed] [Google Scholar]
- Collins J. J., Montelaro R. C., Denny T. P., Ishizaki R., Langlois A. J., Bolognest D. P. Normal chicken cells (chf-) express a surface antigen which cross-reacts with determinants of the major envelope glycoprotein (gp85) of avian myeloblastosis virus. Virology. 1978 May 1;86(1):205–216. doi: 10.1016/0042-6822(78)90021-1. [DOI] [PubMed] [Google Scholar]
- Dowell B. L., Falletta J. M., Moore J. O., Metzgar R. S. Detection and partial characterization of human thymus-leukemia antigens. J Natl Cancer Inst. 1980 Oct;65(4):691–701. doi: 10.1093/jnci/65.4.691. [DOI] [PubMed] [Google Scholar]
- Greaves M. F., Brown G., Rapson N. T., Lister T. A. Antisera to acute lymphoblastic leukemia cells. Clin Immunol Immunopathol. 1975 May;4(1):67–84. doi: 10.1016/0090-1229(75)90041-0. [DOI] [PubMed] [Google Scholar]
- Greaves M., Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim Biophys Acta. 1978 Oct 27;516(2):193–230. doi: 10.1016/0304-419x(78)90008-2. [DOI] [PubMed] [Google Scholar]
- Haynes B. F., Metzgar R. S., Minna J. D., Bunn P. A. Phenotypic characterization of cutaneous T-cell lymphoma. Use of monoclonal antibodies to compare with other malignant T cells. N Engl J Med. 1981 May 28;304(22):1319–1323. doi: 10.1056/NEJM198105283042202. [DOI] [PubMed] [Google Scholar]
- Janossy G., Francis G. E., Capellaro D., Goldstone A. H., Greaves M. F. Cell sorter analysis of leukaemia-associated antigens on human myeloid precursors. Nature. 1978 Nov 9;276(5684):176–178. doi: 10.1038/276176a0. [DOI] [PubMed] [Google Scholar]
- Kersey J. H., LeBien T. W., Abramson C. S., Newman R., Sutherland R., Greaves M. P-24: a human leukemia-associated and lymphohemopoietic progenitor cell surface structure identified with monoclonal antibody. J Exp Med. 1981 Mar 1;153(3):726–731. doi: 10.1084/jem.153.3.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koshiba H., Minowada J., Pressman D. Rabbit antiserum against a non-T, non-B leukemia cell line that carries the Ph1 chromosome (NALM-1): antibody specific to a non-T, non-B acute lymphoblastic leukemia antigen. J Natl Cancer Inst. 1978 Oct;61(4):987–991. [PubMed] [Google Scholar]
- Nakane P K, Pierce G B., Jr Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem. 1966 Dec;14(12):929–931. doi: 10.1177/14.12.929. [DOI] [PubMed] [Google Scholar]
- Newman R. A., Sutherland R., Greaves M. F. The biochemical characterization of a cell surface antigen associated with acute lymphoblastic leukemia and lymphocyte precursors. J Immunol. 1981 May;126(5):2024–2030. [PubMed] [Google Scholar]
- Ritz J., Pesando J. M., Notis-McConarty J., Lazarus H., Schlossman S. F. A monoclonal antibody to human acute lymphoblastic leukaemia antigen. Nature. 1980 Feb 7;283(5747):583–585. doi: 10.1038/283583a0. [DOI] [PubMed] [Google Scholar]
- Ritz J., Pesando J. M., Sallan S. E., Clavell L. A., Notis-McConarty J., Rosenthal P., Schlossman S. F. Serotherapy of acute lymphoblastic leukemia with monoclonal antibody. Blood. 1981 Jul;58(1):141–152. [PubMed] [Google Scholar]