Abstract
The metabolic versatility of the purple nonsulfur photosynethetic bacterial permits the expression of either a phototrophic or a dark aerobic mode of growth. These organism also possess nitrogenase activity which may function under semiaerboic conditions. On the basis of these important properties, the light dependence of nitrogenase function and synthesis in Rhodopseudomonas capsulata was investigated. Nitrogenase activity was strictly dependent on light; no activity was observed in the dark, even when energy (ATP) was supplied by oxidative phosphorylation. It was concluded that the low-potential reducing agent required by the nitrogenase-catalyzed reaction could only be generated by a photochemical reaction. Nitrogenase biosynthesis was also largely dependent on light; however, a small amount of synthesis was observed in resting cells incubated in the dark. Resting cells prepared from dark-grown cultures synthesized nitrogenase at high rates upon illumination. The highest stability of nitrogenase in these resting cells was observed when suspensions were exposed to a diurnal pattern of illumination rather than continuous light. Although nitrogenase function and synthesis are closely coupled to photosynthetic activity, the biosyntheses of bacteriochorophyll and nitrogenase are independent of each other and are most probably subject to different regulatory mechanisms by light.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Atkinson D. E. The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry. 1968 Nov;7(11):4030–4034. doi: 10.1021/bi00851a033. [DOI] [PubMed] [Google Scholar]
- CLAYTON R. K. TOWARD THE ISOLATION OF A PHOTOCHEMICAL REACTION CENTER IN RHODOPSEUDOMONAS SPHEROIDES. Biochim Biophys Acta. 1963 Nov 29;75:312–323. doi: 10.1016/0006-3002(63)90618-8. [DOI] [PubMed] [Google Scholar]
- COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
- Cohen-Bazire G., Kunisawa R. SOME OBSERVATIONS ON THE SYNTHESIS AND FUNCTION OF THE PHOTOSYNTHETIC APPARATUS IN RHODOSPIRILLUM RUBRUM. Proc Natl Acad Sci U S A. 1960 Dec;46(12):1543–1553. doi: 10.1073/pnas.46.12.1543. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daesch G., Mortenson L. E. Effect of ammonia on the synthesis and function of the N 2 -fixing enzyme system in Clostridium pasteurianum. J Bacteriol. 1972 Apr;110(1):103–109. doi: 10.1128/jb.110.1.103-109.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drews G., Lampe H. H., Ladwig R. Die Entwicklung des Photosyntheseapparates in Dunkelkulturen von Rhodopseudomonas capsulata. Arch Mikrobiol. 1969;65(1):12–28. [PubMed] [Google Scholar]
- Fay P. Factors influencing dark nitrogen fixation in a blue-green alga. Appl Environ Microbiol. 1976 Mar;31(3):376–379. doi: 10.1128/aem.31.3.376-379.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hillmer P., Gest H. H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: H2 production by growing cultures. J Bacteriol. 1977 Feb;129(2):724–731. doi: 10.1128/jb.129.2.724-731.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hillmer P., Gest H. H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: production and utilization of H2 by resting cells. J Bacteriol. 1977 Feb;129(2):732–739. doi: 10.1128/jb.129.2.732-739.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kamen M. D., Gest H. Evidence for a Nitrogenase System in the Photosynthetic Bacterium Rhodospirillum rubrum. Science. 1949 Jun 3;109(2840):560–560. doi: 10.1126/science.109.2840.560. [DOI] [PubMed] [Google Scholar]
- Kelley B. C., Meyer C. M., Gandy C., Vignais P. M. Hydrogen recycling by Rhodopseudomonas capsulata. FEBS Lett. 1977 Sep 15;81(2):281–285. doi: 10.1016/0014-5793(77)80535-8. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Meyer J., Kelley B. C., Vignais P. M. Aerobic nitrogen fixation by Rhodopseudomonas capsulata. FEBS Lett. 1978 Jan 15;85(2):224–228. doi: 10.1016/0014-5793(78)80460-8. [DOI] [PubMed] [Google Scholar]
- Meyer J., Kelley B. C., Vignais P. M. Nitrogen fixation and hydrogen metabolism in photosynthetic bacteria. Biochimie. 1978;60(3):245–260. doi: 10.1016/s0300-9084(78)80821-9. [DOI] [PubMed] [Google Scholar]
- Parson W. W., Cogdell R. J. The primary photochemical reaction to bacterial photosynthesis. Biochim Biophys Acta. 1975 Mar 31;416(1):105–149. doi: 10.1016/0304-4173(75)90014-2. [DOI] [PubMed] [Google Scholar]
- Pratt D. C., Frenkel A. W. Studies on Nitrogen Fixation and Photosynthesis of Rhodospirillum Rubrum. Plant Physiol. 1959 May;34(3):333–337. doi: 10.1104/pp.34.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schick H. J. Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum. Arch Mikrobiol. 1971;75(2):89–101. doi: 10.1007/BF00407997. [DOI] [PubMed] [Google Scholar]
- Schön G. Der Einfluss der Kulturbedingungen auf den ATP-, ADP- und AMP-spiegel bei Rhodospirillum rubrum. Arch Mikrobiol. 1969;66(4):348–364. [PubMed] [Google Scholar]
- Weare N. M., Shanmugam K. T. Photoproduction of ammonium ion from N2 in Rhodospirillum rubrum. Arch Microbiol. 1976 Nov 2;110(23):207–213. doi: 10.1007/BF00690229. [DOI] [PubMed] [Google Scholar]
- Weaver P. F., Wall J. D., Gest H. Characterization of Rhodopseudomonas capsulata. Arch Microbiol. 1975 Nov 7;105(3):207–216. doi: 10.1007/BF00447139. [DOI] [PubMed] [Google Scholar]
- Zumft W. G., Castillo F. Regulatory properties of the nitrogenase from Rhodopseudomonas palustris. Arch Microbiol. 1978 Apr 27;117(1):53–60. doi: 10.1007/BF00689351. [DOI] [PubMed] [Google Scholar]