Abstract
Rat skin that survives for long periods of time on immunosuppressed mice becomes resistant to anti-graft serum and remains so for as long as it survives. When long-standing grafts are removed and placed on new immunosuppressed mice, they remain resistant to antiserum for as long as they survive. The acquired resistance to antiserum seems, therefore, to be due to changes in the grafts rather than to changes in their hosts. Furthermore, it was found that the acquisition of resistance is correlated with replacement of graft endothelium by host cells, as demonstrated by the use of immunofluorescent techniques in conjunction with mouse anti-rat serum and rat anti-mouse serum. Evidently, humoral antibodies are able to cause acute damage to skin grafts, and presumably to grafts to other organized tissues, only if they react with antigens of graft endothelium. Long-term grafts that are retransplanted to their original donors or to rats syngeneic with those donors are in most cases rejected, whereas 14-d-old grafts similarly regrafted are in no case rejected. Apparently, the responses of the secondary recipients to the mouse endothelial antigens in long-term grafts lead to destruction of the entire grafts. When long-standing rat skin xenografts are removed and placed on untreated mice syngeneic with the primary hosts, they are in every case rejected, although they survive slightly longer than skin taken directly from rat donors. Rejection is accompanied by a mononuclear infiltrate and is qualitatively indistinguishable from the rejection of freshly prepared rat skin. Clearly, sensitized cells are more efficient than humoral antibody in destroying grafted tissues.
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Selected References
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- Baldamus C. A., McKenzie I. F., Winn H. J., Russell P. S. Acute destruction by humoral antibody of rat skin grafted to mice. J Immunol. 1973 Jun;110(6):1532–1541. [PubMed] [Google Scholar]
- Burdick J. F., Russell P. S., Winn H. J. Sensitivity of long-standing xenografts of rat hearts to humoral antibodies. J Immunol. 1979 Oct;123(4):1732–1735. [PubMed] [Google Scholar]
- Dvorak H. F., Dvorak A. M., Manseau E. J., Wiberg L., Churchill W. H. Fibrin gel investment associated with line 1 and line 10 solid tumor growth, angiogenesis, and fibroplasia in guinea pigs. Role of cellular immunity, myofibroblasts, microvascular damage, and infarction in line 1 tumor regression. J Natl Cancer Inst. 1979 Jun;62(6):1459–1472. [PubMed] [Google Scholar]
- Jooste S. V., Colvin R. B., Soper W. D., Winn H. J. The vascular bed as the primary target in the destruction of skin grafts by antiserum. I. Resistance of freshly placed xenografts of skin to antiserum. J Exp Med. 1981 Nov 1;154(5):1319–1331. doi: 10.1084/jem.154.5.1319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jooste S. V., Winn H. J. Acute destruction of rat skin grafts by alloantisera. J Immunol. 1975 Mar;114(3):933–938. [PubMed] [Google Scholar]
- Jooste S. V., Winn H. J., Russell P. S. Destruction of rat skin grafts by humoral antibody. Transplant Proc. 1973 Mar;5(1):713–716. [PubMed] [Google Scholar]
- Winn H. J. In vivo methods for the assessment of antibody-mediated tumor immunity. Natl Cancer Inst Monogr. 1972 Dec;35:13–18. [PubMed] [Google Scholar]