Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1981 Nov 1;154(5):1475–1488. doi: 10.1084/jem.154.5.1475

Alteration of clonal profile. III. T15 ontogenetic advantages are not sufficient for establishing idiotypic dominance in adoptive transfer

PMCID: PMC2186510  PMID: 6975348

Abstract

We have examined the ontogeny of BALB/c plaque-forming cell (PFC) responses to phosphorylcholine (PC) from fetal and neonatal liver by using the (CBA/N x BALB/c)F1 transplantation model. In this system, thymus-dependent (PC-keyhole limpet hemocyanin) and thymus-independent class 1 (PC-Brucella abortus, PC-lipopolysaccharide) PC antigens stimulate B cell subpopulations, which functionally emerge early after transfer. Responsiveness to a thymus-independent class 2 antigen, C- polysaccharide extract of a Streptococcus pneumoniae mutant, is acquired later. The response to PC antigens tested initially exhibited T15 dominance. Non-T15 clones, which are not expressed to a great degree in normal BALB/c mice, are inherently slow in their rate of maturation; in adoptive transfer, however, they eventually comprise much of the transplanted anti-PC PFC response. Obviously, the advantages the T15 subset has in ontogeny do not result in idiotypic dominance once the immature cells are removed from the intact BALB/c environment. We discuss possible regulatory mechanisms involved in the alteration of the T15+:T15- ratio.

Full Text

The Full Text of this article is available as a PDF (919.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON H. C., McCARTY M. The occurrence in the rabbit of an acute phase protein analogous to human C reactive protein. J Exp Med. 1951 Jan;93(1):25–36. doi: 10.1084/jem.93.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andersson B., Blomgren H. T-cell response to polyvinyl pyrrolidone is linked to maturity of B cells. Nature. 1975 Feb 6;253(5491):476–477. doi: 10.1038/253476a0. [DOI] [PubMed] [Google Scholar]
  3. Bona C., Stein K. E., Lieberman R., Paul W. E. Direct and indirect suppression induced by anti-idiotype antibody in the inulin-bacterial levan antigenic system. Mol Immunol. 1979 Dec;16(12):1093–1101. doi: 10.1016/0161-5890(79)90043-9. [DOI] [PubMed] [Google Scholar]
  4. Bottomly K., Mosier D. E. Mice whose B cells cannot produce the T15 idiotype also lack an antigen-specific helper T cell required for T15 expression. J Exp Med. 1979 Dec 1;150(6):1399–1409. doi: 10.1084/jem.150.6.1399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Briles D. E., Nahm M., Schroer K., Davie J., Baker P., Kearney J., Barletta R. Antiphosphocholine antibodies found in normal mouse serum are protective against intravenous infection with type 3 streptococcus pneumoniae. J Exp Med. 1981 Mar 1;153(3):694–705. doi: 10.1084/jem.153.3.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chesebro B., Metzger H. Affinity labeling of a phosphorylcholine binding mouse myeloma protein. Biochemistry. 1972 Feb 29;11(5):766–771. doi: 10.1021/bi00755a014. [DOI] [PubMed] [Google Scholar]
  7. Claflin J. L. Uniformity in the clonal repertoire for the immune response to phosphorylcholine in mice. Eur J Immunol. 1976 Oct;6(10):669–674. doi: 10.1002/eji.1830061002. [DOI] [PubMed] [Google Scholar]
  8. Clough E. R., Levy D. A., Cebra J. J. CBA/N X BALB/cJ F1 male and female mice can be primed to express quantitatively equivalent secondary anti-phosphocholine responses. J Immunol. 1981 Jan;126(1):387–389. [PubMed] [Google Scholar]
  9. Cosenza H., Köhler H. Specific inhibition of plaque formation to phosphorylcholine by antibody against antibody. Science. 1972 Jun 2;176(4038):1027–1029. doi: 10.1126/science.176.4038.1027. [DOI] [PubMed] [Google Scholar]
  10. Cosenza H., Köhler H. Specific suppression of the antibody response by antibodies to receptors. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2701–2705. doi: 10.1073/pnas.69.9.2701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  12. Forni L., Cazenave P. A., Cosenza H., Forsbeck K., Coutinho A. Expression of V-region-like determinants on Ig-negative precursors in murine fetal liver and bone marrow. Nature. 1979 Jul 19;280(5719):241–243. doi: 10.1038/280241a0. [DOI] [PubMed] [Google Scholar]
  13. Fung J., Köhler H. Late clonal selection and expansion of the TEPC-15 germ-line specificity. J Exp Med. 1980 Nov 1;152(5):1262–1273. doi: 10.1084/jem.152.5.1262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gold E. R., Fudenberg H. H. Chromic chloride: a coupling reagent for passive hemagglutination reactions. J Immunol. 1967 Nov;99(5):859–866. [PubMed] [Google Scholar]
  15. Inman J. K. Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates. J Immunol. 1975 Feb;114(2 Pt 1):704–709. [PubMed] [Google Scholar]
  16. Ivanyi J., Makings C. Allotype analysis of histocompatible and semiallogeneic B cell chimeric chickens. Transplantation. 1980;29(1):25–29. doi: 10.1097/00007890-198001000-00006. [DOI] [PubMed] [Google Scholar]
  17. Kaplan D. R., Quintans J., Köhler H. Clonal dominance: loss and restoration in adoptive transfer. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1967–1970. doi: 10.1073/pnas.75.4.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kaplan D. R., Quintáns J. Alteration of clonal profile. I. Effect of sublethal irradiation on the responses to phosphorylcholine in BALB/c mice. J Exp Med. 1978 Oct 1;148(4):987–995. doi: 10.1084/jem.148.4.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kim B. S., Benewicz B. J. The lack of compensatory increases of cells producing anti-phosphorylcholine antibodies bearing other idiotypes in TEPC-15 idiotype-suppressed inbred and outbred mice. Eur J Immunol. 1980 Mar;10(3):171–175. doi: 10.1002/eji.1830100303. [DOI] [PubMed] [Google Scholar]
  20. Lewis G. K., Goodman J. W. Carrier-directed anti-hapten responses by B-cell subsets. J Exp Med. 1977 Jul 1;146(1):1–10. doi: 10.1084/jem.146.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McKearn J. P., Quintáns J. Idiotype-specific tolerance: preferential alteration of idiotype expression after neonatal exposure to tolerogen. J Immunol. 1980 Apr;124(4):1983–1989. [PubMed] [Google Scholar]
  22. Mond J. J., Lieberman R., Inman J. K., Mosier D. E., Paul W. E. Inability of mice with a defect in B-lymphocyte maturation to respond to phosphorycholine on immunogenic carriers. J Exp Med. 1977 Oct 1;146(4):1138–1142. doi: 10.1084/jem.146.4.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pilarski L. M., Cunningham A. J. Host-derived antibody-forming cells in lethally irradiated mice. J Immunol. 1975 Jan;114(1 Pt 1):138–140. [PubMed] [Google Scholar]
  24. Quintáns J., Cosenza H. Antibody response to phosphorylcholine in vitro. II. Analysis of T-dependent and T-independent responses. Eur J Immunol. 1976 Jun;6(6):399–405. doi: 10.1002/eji.1830060605. [DOI] [PubMed] [Google Scholar]
  25. Quintáns J., Kaplan R. B. Failure of CBA/N mice to respond to thymus-dependent and thymus-independent phosphorylcholine antigens. Cell Immunol. 1978 Jul;38(2):294–301. doi: 10.1016/0008-8749(78)90060-6. [DOI] [PubMed] [Google Scholar]
  26. Quintáns J., Loken M. R., Quan Z. S., Dick R. F., Regueiro B. Alteration of clonal profile. II. Studies on the capacity of BALB/c splenic B cells to perpetuate responsiveness to phosphorylcholine and T 15 idiotypic dominance. Eur J Immunol. 1981 Mar;11(3):236–241. doi: 10.1002/eji.1830110313. [DOI] [PubMed] [Google Scholar]
  27. Quintáns J., McKearn J. P., Kaplan D. B cell heterogeneity. I. A study of B cell subpopulations involved in the reconstitution of an X-linked immune defect of B cell differentiation. J Immunol. 1979 May;122(5):1750–1756. [PubMed] [Google Scholar]
  28. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  29. Sarmiento M., Glasebrook A. L., Fitch F. W. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement. J Immunol. 1980 Dec;125(6):2665–2672. [PubMed] [Google Scholar]
  30. Sher A., Cohn M. Inheritance of an idiotype associated with the immune response of inbred mice to phosphorylcholine. Eur J Immunol. 1972 Aug;2(4):319–326. doi: 10.1002/eji.1830020405. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES