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. 1981 Nov 1;154(5):1290–1304. doi: 10.1084/jem.154.5.1290

Monoclonal antibodies that recognize the product controlled by a gene in the I-J subregion of the mouse H-2 complex

PMCID: PMC2186516  PMID: 6170715

Abstract

The B cell hybridomas producing monoclonal antibodies (E10, D7, F4, H6, and D4) were established by the fusion of P3U1 or NS-1 murine myeloma cell lines and spleen cells of B10.A(5R) mice hyperimmunized with mitomycin C-treated B10.A(3R) spleen and thymus cells. Two types of monoclonal antibodies specific for the products controlled by a gene in the I-Jb subregion of the H-2 complex were characterized: one specific for the private type of I-Jb determinant, the other recognizing the cross-reactive determinant between the I-Jb and I-Jd products. By using these monoclonal reagents, the I-J-encoded product on the antigen- specific suppressor T cells was found to be expressed on their soluble suppressor factors. Furthermore, the I-Jb products were successfully detected not only on the T cell hybridoma with suppressor activity specific for keyhole limpet hemocyanin (KLH), but also on KLH-primed suppressor T cells enriched by antigen-coated petri dishes and concanavalin A-induced thymocyte blasts of C57BL/6 mice by complement- dependent cytotoxic assays and membrane fluorescence techniques.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
  2. Frelinger J. A., Niederhuber J. E., Shreffler D. C. Effects of anti-Ia sera on mitogenic responses. III. Mapping the genes controlling the expression of Ia determinants on concanavalin A-reactive cells to the I-J subregion of the H-2 gene complex. J Exp Med. 1976 Oct 1;144(4):1141–1146. doi: 10.1084/jem.144.4.1141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Germain R. N., Ju S. T., Kipps T. J., Benacerraf B., Dorf M. E. Shared idiotypic determinants on antibodies and T-cell-derived suppressor factor specific for the random terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10. J Exp Med. 1979 Mar 1;149(3):613–622. doi: 10.1084/jem.149.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Greene M. I., Pierres A., Dorf M. E., Benacerraf B. The I-J subregion codes for determinats on suppressor factor(s) which limit the contact sensitivity response to picryl chloride. J Exp Med. 1977 Jul 1;146(1):293–296. doi: 10.1084/jem.146.1.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hayes C. E., Bach F. H. T-cell-specific murine Ia antigens: serology of I-J and I-E subregion specificities. J Exp Med. 1978 Sep 1;148(3):692–703. doi: 10.1084/jem.148.3.692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Klein J. List of congenic lines of mice. I. Lines with differences at alloantigen loci. Transplantation. 1973 Jan;15(1):137–153. doi: 10.1097/00007890-197301000-00021. [DOI] [PubMed] [Google Scholar]
  7. Murphy D. B., Herzenberg L. A., Okumura K., Herzenberg L. A., McDevitt H. O. A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):699–712. doi: 10.1084/jem.144.3.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Niederhuber J. E., Allen P., Mayo L. The expression of Ia antigenic determinants on macrophages required for the in vitro antibody response. J Immunol. 1979 Apr;122(4):1342–1349. [PubMed] [Google Scholar]
  9. Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
  10. Okumura K., Takemori T., Tokuhisa T., Tada T. Specific enrichment of the suppressor T cell bearing I-J determinants: parallel functional and serological characterizations. J Exp Med. 1977 Nov 1;146(5):1234–1245. doi: 10.1084/jem.146.5.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  12. Tada T., Okumura K. The role of antigen-specific T cell factors in the immune response. Adv Immunol. 1979;28:1–87. doi: 10.1016/s0065-2776(08)60799-3. [DOI] [PubMed] [Google Scholar]
  13. Tada T., Takemori T., Okumura K., Nonaka M., Tokuhisa T. Two distinct types of helper T cells involved in the secondary antibody response: independent and synergistic effects of Ia- and Ia+ helper T cells. J Exp Med. 1978 Feb 1;147(2):446–458. doi: 10.1084/jem.147.2.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tada T., Taniguchi M., David C. S. Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. J Exp Med. 1976 Sep 1;144(3):713–725. doi: 10.1084/jem.144.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taniguchi M., Miller J. F. Enrichment of specific suppressor T cells and characterization of their surface markers. J Exp Med. 1977 Nov 1;146(5):1450–1454. doi: 10.1084/jem.146.5.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Taniguchi M., Saito T., Tada T. Antigen-specific suppressive factor produced by a transplantable I-J bearing T-cell hybridoma. Nature. 1979 Apr 5;278(5704):555–558. doi: 10.1038/278555a0. [DOI] [PubMed] [Google Scholar]
  17. Taniguchi M., Saito T., Takei I., Tokuhisa T. Presence of interchain disulfide bonds between two gene products that compose the secreted form of an antigen-specific suppressor factor. J Exp Med. 1981 Jun 1;153(6):1672–1677. doi: 10.1084/jem.153.6.1672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taniguchi M., Takei I., Tada T. Functional and molecular organisation of an antigen-specific suppressor factor from a T-cell hybridoma. Nature. 1980 Jan 10;283(5743):227–228. doi: 10.1038/283227a0. [DOI] [PubMed] [Google Scholar]
  19. Theze J., Kapp J. A., Benacerraf B. Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) III. Immunochemical properties of the GAT-specific suppressive factor. J Exp Med. 1977 Apr 1;145(4):839–856. doi: 10.1084/jem.145.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thèze J., Waltenbaugh C., Dorf M. E., Benacerraf B. Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyrosine50 (GT) II. Presence of I-J determinants on the GT-suppressive factor. J Exp Med. 1977 Jul 1;146(1):287–292. doi: 10.1084/jem.146.1.287. [DOI] [PMC free article] [PubMed] [Google Scholar]

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