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. 1981 Dec 1;154(6):1910–1921. doi: 10.1084/jem.154.6.1910

Two separate genes regulate self-Ia and carrier recognition in H-2- restricted helper factors secreted by hybridoma cells

PMCID: PMC2186535  PMID: 6172536

Abstract

H-2 heterologous T cell hybridomas were used to study the genetic control of dual, anti-nominal antigen and anti-self H-2 specificity of H-2 restricted T cell factors. Each of four hybridoma clones produced two helper factors. One was restricted for the Ia type of the normal T cell partner (H-2b), whereas the other was restricted for the ia type of the lymphoma partner (H-2k) of the somatic hybrid. This was shown by affinity separation on parental type spleen cells and on monoclonal anti-I-A-Sepharose. Both factors had carrier (chicken gamma globulin; CGG)-specific helper effect, and both bound to anti-VH-315-Sepharose. Because the lymphoma (BW-5147) partner could not contribute a CGG- specific locus, the H-2k-restricted, CGG-specific factor had to be the product of segregating anti-nominal and anti-self loci. This suggests that dual specificity is due to two independent loci and support the validity of dual recognition concepts. Anti-self specificity was associated with homologous Ia alloantigens in the individual factors. Therefore, Ia and anti-self might be linked. Implications of the major histocompatibility complex or VH nature of anti-self receptors and the relationship of T cell factors and receptors was discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach B. A., Greene M. I., Benacerraf B., Nisonoff A. Mechanisms of regulation of cell-mediated immunity. IV. Azobenzenearsonate-specific suppressor factor(s) bear cross-reactive idiotypic determinants the expression of which is linked to the heavy-chain allotype linkage group of genes. J Exp Med. 1979 May 1;149(5):1084–1098. doi: 10.1084/jem.149.5.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ben-Neriah Y., Lonai P., Gavish M., Givol D. Preparation and characterization of antibodies to the lambda chain variable region (Vlambda) of mouse immunoglobulins. Eur J Immunol. 1978 Nov;8(11):792–796. doi: 10.1002/eji.1830081108. [DOI] [PubMed] [Google Scholar]
  3. Ben-Neriah Y., Lonai P., Givol D. Preparation and characterization of anti-framework antibodies to the kappa-chain variable region (V kappa) of mouse immunoglobulins. J Immunol. 1980 Feb;124(2):691–695. [PubMed] [Google Scholar]
  4. Ben-Neriah Y., Wuilmart C., Lonai P., Givol D. Preparation and characterization of anti-framework antibodies to the heavy chain variable region (VH) of mouse immunoglobulins. Eur J Immunol. 1978 Nov;8(11):797–801. doi: 10.1002/eji.1830081109. [DOI] [PubMed] [Google Scholar]
  5. Binz H., Frischknecht H., Mercolli C., Dunst S., Wigzell H. Binding of purified, soluble major histocompatibility complex polypeptide chains onto isolated T-cell receptors. I. Reactivity against allo- and self-determinants. J Exp Med. 1979 Nov 1;150(5):1084–1095. doi: 10.1084/jem.150.5.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eichmann K., Ben-Neriah Y., Hetzelberger D., Polke C., Givol D., Lonai P. Correlated expression of VH framework and VH idiotypic determinants on T helper cells and on functionally undefined T cells binding group A streptococcal carbohydrate. Eur J Immunol. 1980 Feb;10(2):105–112. doi: 10.1002/eji.1830100207. [DOI] [PubMed] [Google Scholar]
  7. Eichmann K. Expression and function of idiotypes of lymphocytes. Adv Immunol. 1978;26:195–254. doi: 10.1016/s0065-2776(08)60231-x. [DOI] [PubMed] [Google Scholar]
  8. Germain R. N., Ju S. T., Kipps T. J., Benacerraf B., Dorf M. E. Shared idiotypic determinants on antibodies and T-cell-derived suppressor factor specific for the random terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10. J Exp Med. 1979 Mar 1;149(3):613–622. doi: 10.1084/jem.149.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Janeway C. A., Wigzell H., Binz H. Two different VH gene products make up the T-cell receptors. Scand J Immunol. 1976;5(9):993–1001. doi: 10.1111/j.1365-3083.1976.tb03051.x. [DOI] [PubMed] [Google Scholar]
  10. Kappler J. W., Skidmore B., White J., Marrack P. Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J Exp Med. 1981 May 1;153(5):1198–1214. doi: 10.1084/jem.153.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lonai P., Puri J., Bitton S., Ben-Neriah Y., Givol D., Hämmerling G. J. H-2-restricted helper factor secreted by clone hybridoma cells. J Exp Med. 1981 Sep 1;154(3):942–951. doi: 10.1084/jem.154.3.942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lonai P., Puri J., Hämmerling G. H-2-restricted antigen binding by a hybridoma clone that produces antigen-specific helper factor. Proc Natl Acad Sci U S A. 1981 Jan;78(1):549–553. doi: 10.1073/pnas.78.1.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Neriah Y. B., Givol D., Lonai P., Simon M. M., Eichmann K. Allotype-linked genetic control of a polymorphic VH framework framework determinant on mouse T-helper cell receptors. Nature. 1980 May 22;285(5762):257–259. doi: 10.1038/285257a0. [DOI] [PubMed] [Google Scholar]
  14. Puri J., Ben-Neriah Y., Givol D., Lonai P. Antibodies to immunoglobulin heavy chain variable regions protect helper cells from specific suicide by radiolabeled antigen. Eur J Immunol. 1980 Apr;10(4):281–284. doi: 10.1002/eji.1830100411. [DOI] [PubMed] [Google Scholar]
  15. Puri J., Lonai P. Mechanism of antigen binding by T cells. H-2(I-A)-restricted binding of antigen plus Ia by helper cells. Eur J Immunol. 1980 Apr;10(4):273–281. doi: 10.1002/eji.1830100410. [DOI] [PubMed] [Google Scholar]
  16. Shearer G. M. Cell-mediated cytotoxicity to trinitrophenyl-modified syngeneic lymphocytes. Eur J Immunol. 1974 Aug;4(8):527–533. doi: 10.1002/eji.1830040802. [DOI] [PubMed] [Google Scholar]
  17. Tada T., Okumura K. The role of antigen-specific T cell factors in the immune response. Adv Immunol. 1979;28:1–87. doi: 10.1016/s0065-2776(08)60799-3. [DOI] [PubMed] [Google Scholar]
  18. Zinkernagel R. M., Callahan G. N., Althage A., Cooper S., Klein P. A., Klein J. On the thymus in the differentiation of "H-2 self-recognition" by T cells: evidence for dual recognition? J Exp Med. 1978 Mar 1;147(3):882–896. doi: 10.1084/jem.147.3.882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zinkernagel R. M., Doherty P. C. Restriction of in vitro T cell-mediated cytotoxicity in lymphocytic choriomeningitis within a syngeneic or semiallogeneic system. Nature. 1974 Apr 19;248(5450):701–702. doi: 10.1038/248701a0. [DOI] [PubMed] [Google Scholar]

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