Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1981 Dec 1;154(6):1881–1898. doi: 10.1084/jem.154.6.1881

Adherent Ia+ murine tumor lines with characteristics of dendritic cells. I. Morphology, surface phenotype, and induction of syngeneic mixed lymphocyte reactions

PMCID: PMC2186540  PMID: 6459398

Abstract

Several adherent cell lines that express surface Ia antigens have been cloned from the P388 leukemia. These cell lines express many morphological and functional similarities to dendritic cells of the spleen. Adherent clones express H-2 gene products from the I and D regions, but do not possess Fc or C3b receptors or surface Ig. The cytoplasm of the adherent P388 clones has an abundance of mitochondria but few phagolysosomes. The adherent clones all strongly stimulate the proliferation of syngeneic or allogeneic T lymphocytes in the absence of antigen; however, cytotoxic T cells directed against the stimulators are induced only during allogeneic stimulation. Nonadherent clones of P388, which also express Ia antigens, do not stimulate either allogeneic or syngeneic T cell proliferation. The isolation of Ia- bearing adherent cell lines makes it possible to study Ia-mediated events in both antigen-dependent and independent immune systems using homogeneous accessory cell populations.

Full Text

The Full Text of this article is available as a PDF (1.7 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burger R., Shevach E. M. Monoclonal antibodies to guinea pig Ia antigens. II. Effect on alloantigen-, antigen-, and mitogen-induced T lymphocyte proliferation in vitro. J Exp Med. 1980 Oct 1;152(4):1011–1023. doi: 10.1084/jem.152.4.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hausman P. B., Raff H. V., Gilbert R. C., Picker L. J., Stobo J. D. T cells and macrophages involved in the autologous mixed lymphocyte reaction are required for the response to conventional antigen. J Immunol. 1980 Sep;125(3):1374–1379. [PubMed] [Google Scholar]
  3. Hausman P. B., Stites D. P., Stobo J. D. Antigen-reactive T cells can be activated buy autologous macrophages in the absence of added antigen. J Exp Med. 1981 Feb 1;153(2):476–481. doi: 10.1084/jem.153.2.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  5. KAPLOW L. S. SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. Blood. 1965 Aug;26:215–219. [PubMed] [Google Scholar]
  6. Koren H. S., Handwerger B. S., Wunderlich J. R. Identification of macrophage-like characteristics in a cultured murine tumor line. J Immunol. 1975 Feb;114(2 Pt 2):894–897. [PubMed] [Google Scholar]
  7. Kuntz M. M., Innes J. B., Weksler M. E. Lymphocyte transformation induced by autologous cells. IV. Human T-lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes. J Exp Med. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Li C. Y., Lam K. W., Yam L. T. Esterases in human leukocytes. J Histochem Cytochem. 1973 Jan;21(1):1–12. doi: 10.1177/21.1.1. [DOI] [PubMed] [Google Scholar]
  9. Longo D. L., Schwartz R. H. Inhibition of antigen-induced proliferation of T cells from radiation-induced bone marrow chimeras by a monoclonal antibody directed against an Ia determinant on the antigen-presenting cell. Proc Natl Acad Sci U S A. 1981 Jan;78(1):514–518. doi: 10.1073/pnas.78.1.514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Meltzer M. S., Oppenheim J. J. Bidirectional amplification of macrophage-lymphocyte interactions: enhanced lymphocyte activation factor production by activated adherent mouse peritoneal cells. J Immunol. 1977 Jan;118(1):77–82. [PubMed] [Google Scholar]
  11. Niederhuber J. E., Allen P. Role of I-region gene products in macrophage induction of an antibody response. II. Restriction at the level of T cell in recognition of I-J-subregion macrophage determinants. J Exp Med. 1980 May 1;151(5):1103–1113. doi: 10.1084/jem.151.5.1103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Niederhuber J. E., Allen P. The role of I region gene products in macrophage induction of an antibody response. I. Ability of anti-I-J region sera to block macrophage function. J Immunol. 1980 Aug;125(2):799–806. [PubMed] [Google Scholar]
  13. Nussenzweig M. C., Steinman R. M., Gutchinov B., Cohn Z. A. Dendritic cells are accessory cells for the development of anti-trinitrophenyl cytotoxic T lymphocytes. J Exp Med. 1980 Oct 1;152(4):1070–1084. doi: 10.1084/jem.152.4.1070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Opelz G., Kiuchi M., Takasugi M., Terasaki P. I. Autologous stimulation of human lymphocyte subpopulation. J Exp Med. 1975 Nov 1;142(5):1327–1333. doi: 10.1084/jem.142.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Phillips M. L., Parker J. W., Frelinger J. A., O'Brien R. L. Characterization of responding cells in oxidative mitogen stimulation. II. Identification of an Ia-bearing adherent accessory cell. J Immunol. 1980 Jun;124(6):2700–2707. [PubMed] [Google Scholar]
  16. Raff H. V., Picker L. J., Stobo J. D. Macrophage heterogeneity in man. A subpopulation of HLA-DR-bearing macrophages required for antigen-induced T cell activation also contains stimulators for autologous-reactive T cells. J Exp Med. 1980 Sep 1;152(3):581–593. doi: 10.1084/jem.152.3.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schwartz R. H., David C. S., Sachs D. H., Paul W. E. T lymphocyte-enriched murine peritoneal exudate cells. III. Inhibition of antigen-induced T lymphocyte Proliferation with anti-Ia antisera. J Immunol. 1976 Aug;117(2):531–540. [PubMed] [Google Scholar]
  18. Shevach E. M., Paul W. E., Green I. Histocompatibility-linked immune response gene function in guinea pigs. Specific inhibition of antigen-induced lymphocyte proliferation by alloantisera. J Exp Med. 1972 Nov 1;136(5):1207–1221. doi: 10.1084/jem.136.5.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]
  20. Steinman R. M., Cohn Z. A. Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution. J Exp Med. 1973 May 1;137(5):1142–1162. doi: 10.1084/jem.137.5.1142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Steinman R. M., Nussenzweig M. C. Dendritic cells: features and functions. Immunol Rev. 1980;53:127–147. doi: 10.1111/j.1600-065x.1980.tb01042.x. [DOI] [PubMed] [Google Scholar]
  22. Stingl G., Katz S. I., Clement L., Green I., Shevach E. M. Immunologic functions of Ia-bearing epidermal Langerhans cells. J Immunol. 1978 Nov;121(5):2005–2013. [PubMed] [Google Scholar]
  23. Sunshine G. H., Katz D. R., Feldmann M. Dendritic cells induce T cell proliferation to synthetic antigens under Ir gene control. J Exp Med. 1980 Dec 1;152(6):1817–1822. doi: 10.1084/jem.152.6.1817. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES