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. 1982 Jan 1;155(1):126–139. doi: 10.1084/jem.155.1.126

Two distinct allotypic determinants on the antigen-specific suppressor and enhancing T cell factors that are encoded by genes linked to the immunoglobulin heavy chain locus

PMCID: PMC2186577  PMID: 6172538

Abstract

The alloantiserum was raised in BALB/c (H-2d, Igh-1a) mice hyperimmunized with spleen cells of Igh allotype congenic mice, CB-20 (H-2d, Igh-1b). It was found to define the new allotypic determinants (distinct from B cell Igh constant region determinants: Igh allotype) expressed only on a small population of T cells belonging to the Thy-1 dull-stained Lyt-2- or Lyt-2+ population of Igh-1b mice. Genes coding for the determinants were shown to be accommodated somewhere in the right side of the Igh variable region gene (Igh-V) cluster, as the antibody activity was completely absorbed with BAB-14 thymocytes. It was also demonstrated that the products detected by the antiserum represent the allotypic determinants (probably constant region determinants) on the antigen-binding moiety of the antigen-specific augmenting (TaF) and suppressor (TsF) T cell factors. Moreover, determinants on TsF were found to be distinct from those on TaF. Therefore, it can be suggested that the two genes coding for the T cell allodeterminants (distinct from those of the B cell Igh) are located in the right side of the B cell Igh-V on the 12th chromosome, and that both encode the antigen-recognition units of the functionally distinct T cell factors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
  2. Bach B. A., Greene M. I., Benacerraf B., Nisonoff A. Mechanisms of regulation of cell-mediated immunity. IV. Azobenzenearsonate-specific suppressor factor(s) bear cross-reactive idiotypic determinants the expression of which is linked to the heavy-chain allotype linkage group of genes. J Exp Med. 1979 May 1;149(5):1084–1098. doi: 10.1084/jem.149.5.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Binz H., Wigzell H. Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens. J Exp Med. 1975 Jul 1;142(1):197–211. doi: 10.1084/jem.142.1.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eshhar Z., Apte R. N., Löwy I., Ben-Neriah Y., Givol D., Mozes E. T-cell hybridoma bearing heavy chain variable region determinants producing (T,G)-A--L-specific helper factor. Nature. 1980 Jul 17;286(5770):270–272. doi: 10.1038/286270a0. [DOI] [PubMed] [Google Scholar]
  5. Germain R. N., Ju S. T., Kipps T. J., Benacerraf B., Dorf M. E. Shared idiotypic determinants on antibodies and T-cell-derived suppressor factor specific for the random terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10. J Exp Med. 1979 Mar 1;149(3):613–622. doi: 10.1084/jem.149.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  7. Kontiainen S., Feldmann M. Suppressor-cell induction in vitro. IV. Target of antigen-specific suppressor factor and its genetic relationships. J Exp Med. 1978 Jan 1;147(1):110–122. doi: 10.1084/jem.147.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Krawinkel U., Cramer M., Melchers I., Imanishi-Kari T., Rajewsky K. Isolated hapten-binding receptors of sensitized lymphocytes. III. Evidence for idiotypic restriction of T-cell receptors. J Exp Med. 1978 May 1;147(5):1341–1347. doi: 10.1084/jem.147.5.1341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ledbetter J. A., Rouse R. V., Micklem H. S., Herzenberg L. A. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280–295. doi: 10.1084/jem.152.2.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Owen F. L. A mature T lymphocyte marker closely linked to Igh-1 that is expressed on the precursor for the suppressor T cell regulating a primary response to SRBC. J Immunol. 1980 Mar;124(3):1411–1417. [PubMed] [Google Scholar]
  11. Owen F. L., Finnegan A., Gates E. R., Gottlieb P. D. A mature T lymphocyte subpopulation marker closely linked to the Ig-1 allotype Ch locus. Eur J Immunol. 1979 Dec;9(12):948–955. doi: 10.1002/eji.1830091208. [DOI] [PubMed] [Google Scholar]
  12. Owen F. L., Riblet R., Taylor B. A. The T suppressor cell alloantigen Tsud maps near immunoglobulin allotype genes and may be an heavy chain constant-region marker on a T cell receptor. J Exp Med. 1981 Apr 1;153(4):801–810. doi: 10.1084/jem.153.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tada T., Hayakawa K., Okumura K., Taniguchi M. Coexistence of variable region of immunoglobulin heavy chain and I region gene products on antigen-specific suppressor T cells and suppressor T cell factor. A minimal model of functional antigen receptor of T cells. Mol Immunol. 1980 Jul;17(7):867–875. doi: 10.1016/0161-5890(80)90035-8. [DOI] [PubMed] [Google Scholar]
  14. Tada T., Taniguchi M., David C. S. Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. J Exp Med. 1976 Sep 1;144(3):713–725. doi: 10.1084/jem.144.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taniguchi M., Hayakawa K., Tada T. Properties of antigen-specific suppressive T cell factor in the regulation of antibody response of the mouse. II. In vitro activity and evidence for the I region gene product. J Immunol. 1976 Feb;116(2):542–548. [PubMed] [Google Scholar]
  16. Taniguchi M., Saito T., Tada T. Antigen-specific suppressive factor produced by a transplantable I-J bearing T-cell hybridoma. Nature. 1979 Apr 5;278(5704):555–558. doi: 10.1038/278555a0. [DOI] [PubMed] [Google Scholar]
  17. Taniguchi M., Saito T., Takei I., Tokuhisa T. Presence of interchain disulfide bonds between two gene products that compose the secreted form of an antigen-specific suppressor factor. J Exp Med. 1981 Jun 1;153(6):1672–1677. doi: 10.1084/jem.153.6.1672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taniguchi M., Takei I., Tada T. Functional and molecular organisation of an antigen-specific suppressor factor from a T-cell hybridoma. Nature. 1980 Jan 10;283(5743):227–228. doi: 10.1038/283227a0. [DOI] [PubMed] [Google Scholar]
  19. Theze J., Kapp J. A., Benacerraf B. Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) III. Immunochemical properties of the GAT-specific suppressive factor. J Exp Med. 1977 Apr 1;145(4):839–856. doi: 10.1084/jem.145.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tokuhisa T., Taniguchi M., Okumura K., Tada T. An antigen-specific I region gene product that augments the antibody response. J Immunol. 1978 Feb;120(2):414–421. [PubMed] [Google Scholar]

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