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. 1982 Feb 1;155(2):524–534. doi: 10.1084/jem.155.2.524

Genetic mapping of ecotropic murine leukemia virus-inducing loci in six inbred strains

PMCID: PMC2186586  PMID: 6276492

Abstract

Mendelian segregation analysis was used to map chromosomal genes for the induction of endogenous N- and B-tropic ecotropic retroviruses (V loci) in high and low leukemic mouse strains. Patterns of virus expression were determined for mice of various inbred strains, congenic lines carrying single V loci, and the linkage testing stocks used in mapping studies. Segregation analysis resulted in the genetic mapping of V loci from six inbred strains to five mouse chromosomes. The V locus of A/J was mapped to chromosome 5 and shown to be allelic with that of BALB/cJ and C3H/HeJ (Cv); this suggests that Cv-represents a stable ancestral V locus present in Bagg albino stocks before the separation of inbred lines. The single, poorly inducible V locus of C57BL/10J and one of the four high virus loci of C58/Lw were mapped to the same region of chromosome 8 and may represent an allelic pair with different patterns of expression. An N-tropic V locus of the SEA/GnJ mouse was mapped to chromosome 9, and one of the three V loci of C3H/FgLw was mapped to chromosome 7. The endogenous B-tropic virus of B10.BR/SgLi was mapped to chromosome 11. These studies provide further evidence that endogenous ecotropic V loci are present at different chromosomal sites in unrelated mouse strains and emphasize the role of germ line reinfections in the generation of this diversity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chattopadhyay S. K., Lander M. R., Rowe W. P. Close similarity between endogenous ecotropic virus of Mus musculus molossinus and AKR virus. J Virol. 1980 Nov;36(2):499–505. doi: 10.1128/jvi.36.2.499-505.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chattopadhyay S. K., Rowe W. P., Teich N. M., Lowy D. R. Definitive evidence that the murine C-type virus inducing locus Akv-1 is viral genetic material. Proc Natl Acad Sci U S A. 1975 Mar;72(3):906–910. doi: 10.1073/pnas.72.3.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gazdar A. F., Oie H., Lalley P., Moss W. W., Minna J. D. Identification of mouse chromosomes required for murine leukemia virus replication. Cell. 1977 Aug;11(4):949–956. doi: 10.1016/0092-8674(77)90306-3. [DOI] [PubMed] [Google Scholar]
  4. Hartley J. W., Rowe W. P. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975 May;65(1):128–134. doi: 10.1016/0042-6822(75)90013-6. [DOI] [PubMed] [Google Scholar]
  5. Hartley J. W., Rowe W. P. Naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class. J Virol. 1976 Jul;19(1):19–25. doi: 10.1128/jvi.19.1.19-25.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ihle J. N., Joseph D. R., Domotor J. J., Jr Genetic linkage of C3H/HeJ and BALB/c endogenous ecotropic C-type viruses to phosphoglucomutase-1 on chromosome 5. Science. 1979 Apr 6;204(4388):71–73. doi: 10.1126/science.219476. [DOI] [PubMed] [Google Scholar]
  7. Ihle J. N., Joseph D. R. Genetic analysis of the endogenous C3H murine leukemia virus genome: evidence for one locus unlinked to the endogenous murine leukemia virus genome of C57BL/6 mice. Virology. 1978 Jun 15;87(2):298–306. doi: 10.1016/0042-6822(78)90135-6. [DOI] [PubMed] [Google Scholar]
  8. Ihle J. N., Joseph D. R. Serological and virological analysis of NIH (NIH X AKR) mice: evidence for three AKR murine leukemia virus loci. Virology. 1978 Jun 15;87(2):287–297. doi: 10.1016/0042-6822(78)90134-4. [DOI] [PubMed] [Google Scholar]
  9. Kozak C. A., Rowe W. P. Genetic mapping of the ecotropic virus-inducing locus Akv-2 of the AKR mouse. J Exp Med. 1980 Nov 1;152(5):1419–1423. doi: 10.1084/jem.152.5.1419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kozak C. A., Rowe W. P. Genetic mapping of xenotropic murine leukemia virus-inducing loci in five mouse strains. J Exp Med. 1980 Jul 1;152(1):219–228. doi: 10.1084/jem.152.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LAW L. W. Present status of nonviral factors in the etiology of reticular neoplasms of the mouse. Ann N Y Acad Sci. 1957 Oct 21;68(2):616–635. doi: 10.1111/j.1749-6632.1957.tb56109.x. [DOI] [PubMed] [Google Scholar]
  12. Lander M. R., Moll B., Rowe W. P. A procedure for culture of cells from mouse tail biopsies: brief communication. J Natl Cancer Inst. 1978 Feb;60(2):477–478. [PubMed] [Google Scholar]
  13. Moll B., Hartley J. W., Rowe W. P. Induction of B-tropic and N-tropic murine leukemia virus from B10.BR/SgLi mouse embryo cell lines by 5-iodo-2'-deoxyuridine. J Natl Cancer Inst. 1979 Jul;63(1):213–217. [PubMed] [Google Scholar]
  14. Rowe W. P., Hartley J. W., Bremner T. Genetic mapping of a murine leukemia virus-inducing locus of AKR mice. Science. 1972 Nov 24;178(4063):860–862. doi: 10.1126/science.178.4063.860. [DOI] [PubMed] [Google Scholar]
  15. Rowe W. P., Kozak C. A. Germ-line reinsertions of AKR murine leukemia virus genomes in Akv-1 congenic mice. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4871–4874. doi: 10.1073/pnas.77.8.4871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  17. Rowe W. P. Studies of genetic transmission of murine leukemia virus by AKR mice. I. Crosses with Fv-1 n strains of mice. J Exp Med. 1972 Nov 1;136(5):1272–1285. doi: 10.1084/jem.136.5.1272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Staats J. Standardized nomenclature for inbred strains of mice: seventh listing for the International Committee on Standardized Genetic Nomenclature for Mice. Cancer Res. 1980 Jul;40(7):2083–2128. [PubMed] [Google Scholar]
  19. Steffen D. L., Bird S., Weinberg R. A. Evidence for the Asiatic origin of endogenous AKR-type murine leukemia proviruses. J Virol. 1980 Sep;35(3):824–835. doi: 10.1128/jvi.35.3.824-835.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stephenson J. R., Aaronson S. A. A genetic locus for inducibility of C-type in BALB-c cells: the effect of a nonlinked regulatory gene on detection of virus after chemical activation. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2798–2801. doi: 10.1073/pnas.69.10.2798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stephenson J. R., Aaronson S. A. Segregation of loci for C-type virus induction in strains of mice with high and low incidence of leukemia. Science. 1973 May 25;180(4088):865–866. doi: 10.1126/science.180.4088.865. [DOI] [PubMed] [Google Scholar]

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