Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1982 Feb 1;155(2):445–459. doi: 10.1084/jem.155.2.445

Ia antigen-bearing B cell tumor lines can present protein antigen and alloantigen in a major histocompatibility complex-restricted fashion to antigen-reactive T cells

PMCID: PMC2186598  PMID: 6460073

Abstract

Several Ia-positive BALB/c B cell tumor lines were screened for their ability to present alloantigen and protein antigens to alloreactive and antigen-reactive T cells. Of six Ia-positive tumor lines studied, three were found to be effective as antigen presenting cells (APC). Indeed, on a per cell basis, one of the stimulatory lines, A20.3, was substantially more effective than whole spleen cells. The other three lines, although Ia-positive, were nonstimulatory. A20.3 was chosen for further study. This tumor appeared to behave like the conventional APC because (a) the tumor cells presented alloantigen, (b) they presented protein antigen in an MHC-restricted fashion to both primed donor T cells and to long-term continuous T cell lines, (c) alloantigen presentation was blocked by the inclusion of an anti-Ia antibody in the culture system, and (d) A20.3 cells could be effectively pulsed with antigen, although the continuous presence of antigen in the culture system resulted in a superior response. The addition of an exogenous source of interleukin 1 proved necessary to obtain an alloreactive but not an antigen-specific T cell response, although its inclusion did enhance the magnitude of antigen-stimulated proliferation. These tumor cells should prove useful in studying the biochemical events that occur during antigen processing and the requirements for T cell triggering by processed antigen in association with Ia molecules.

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmann G. B., Nadler P. I., Birnkrant A., Hodes R. J. T cell recognition in the mixed lymphocyte response. I. Non-T, radiation-resistant splenic adherent cells are the predominant stimulators in the murine mixed lymphocyte reaction. J Immunol. 1979 Aug;123(2):903–909. [PubMed] [Google Scholar]
  2. Andersson J., Schreier M. H., Melchers F. T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1612–1616. doi: 10.1073/pnas.77.3.1612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chesnut R. W., Grey H. M. Studies on the capacity of B cells to serve as antigen-presenting cells. J Immunol. 1981 Mar;126(3):1075–1079. [PubMed] [Google Scholar]
  4. Hahn E., Nowack H., GOTZE D., Timpl R. H-2-linked genetic control of antibody response to soluble calf skin collagen in mice. Eur J Immunol. 1975 Apr;5(4):288–291. doi: 10.1002/eji.1830050415. [DOI] [PubMed] [Google Scholar]
  5. Hedrick S. M., Watson J. Genetic control of the immune response to collagen. III. Coordinate restriction of cellular cooperation and antigen responsiveness by thymus-directed maturation. J Immunol. 1980 Oct;125(4):1782–1788. [PubMed] [Google Scholar]
  6. Kim K. J., Kanellopoulos-Langevin C., Merwin R. M., Sachs D. H., Asofsky R. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J Immunol. 1979 Feb;122(2):549–554. [PubMed] [Google Scholar]
  7. Kim K. J., Weinbaum F. I., Mathieson B. J., McKeever P. E., Asofsky R. Characteristics of BALB/C T cell lymphomas grown as continuous in vitro lines. J Immunol. 1978 Jul;121(1):339–344. [PubMed] [Google Scholar]
  8. Kimoto M., Fathman C. G. Antigen-reactive T cell clones. I. Transcomplementing hybrid I-A-region gene products function effectively in antigen presentation. J Exp Med. 1980 Oct 1;152(4):759–770. doi: 10.1084/jem.152.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller J. F., Vadas M. A., Whitelaw A., Gamble J. H-2 gene complex restricts transfer of delayed-type hypersensitivity in mice. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5095–5098. doi: 10.1073/pnas.72.12.5095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mizel S. B., Mizel D. Purification to apparent homogeneity of murine interleukin 1. J Immunol. 1981 Mar;126(3):834–837. [PubMed] [Google Scholar]
  11. Mizel S. B., Rosenstreich D. L., Oppenheim J. J. Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D. Cell Immunol. 1978 Sep 15;40(1):230–235. doi: 10.1016/0008-8749(78)90330-1. [DOI] [PubMed] [Google Scholar]
  12. Rogoff T. M., Lipsky P. E. Characterization of isolated guinea pig Kupffer cells: accessory cell function in mitogen-induced T lymphocyte activation. J Immunol. 1979 Nov;123(5):1920–1927. [PubMed] [Google Scholar]
  13. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schwartz R. H., Dorf M. E., Benacerraf B., Paul W. E. The requirement for two complementing Ir-GLphi immune response genes in the T-lymphocyte proliferative response to poly-(Glu53Lys36Phe11). J Exp Med. 1976 Apr 1;143(4):897–905. doi: 10.1084/jem.143.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sprent J. Role of the H-2 complex in induction of T helper cells in vivo. I. Antigen-specific selection of donor T cells to sheep erythrocytes in irradiated mice dependent upon sharing of H-2 determinants between donor and host. J Exp Med. 1978 Aug 1;148(2):478–489. doi: 10.1084/jem.148.2.478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sredni B., Tse H. Y., Schwartz R. H. Direct cloning and extended culture of antigen-specific MHC-restricted, proliferating T lymphocytes. Nature. 1980 Feb 7;283(5747):581–583. doi: 10.1038/283581a0. [DOI] [PubMed] [Google Scholar]
  17. Steinman R. M., Nussenzweig M. C. Dendritic cells: features and functions. Immunol Rev. 1980;53:127–147. doi: 10.1111/j.1600-065x.1980.tb01042.x. [DOI] [PubMed] [Google Scholar]
  18. Stingl G., Katz S. I., Green I., Shevach E. M. The functional role of Langerhans cells. J Invest Dermatol. 1980 May;74(5):315–318. doi: 10.1111/1523-1747.ep12543548. [DOI] [PubMed] [Google Scholar]
  19. Sunshine G. H., Katz D. R., Feldmann M. Dendritic cells induce T cell proliferation to synthetic antigens under Ir gene control. J Exp Med. 1980 Dec 1;152(6):1817–1822. doi: 10.1084/jem.152.6.1817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Swierkosz J. E., Rock K., Marrack P., Kappler J. W. The role of H-2 linked genes in helper T-cell function. II. Isolation on antigen-pulsed macrophages of two separate populations of F1 helper T cells each specific for antigen and one set of parental H-2 products. J Exp Med. 1978 Feb 1;147(2):554–570. doi: 10.1084/jem.147.2.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Waldmann H. The influence of the major histocompatibility complex on the function of T-helper cells in antibody formation. Immunol Rev. 1978;42:202–223. doi: 10.1111/j.1600-065x.1978.tb00263.x. [DOI] [PubMed] [Google Scholar]
  22. Waldron J. A., Jr, Horn R. G., Rosenthal A. S. Antigen-induced proliferation of guinea pig lymphocytes in vitro: obligatory role of macrophages in the recognition of antigen by immune T-lymphocytes. J Immunol. 1973 Jul;111(1):58–64. [PubMed] [Google Scholar]
  23. Weinberger O., Herrmann S. H., Mescher M. F., Benacerraf B., Burakoff S. J. Cellular interactions in the generation of cytolytic T lymphocyte responses: role of Ia-positive splenic adherent cells in presentation in H-2 antigen. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6091–6095. doi: 10.1073/pnas.77.10.6091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weinberger O., Herrmann S., Mescher M. F., Benacerraf B., Burakoff S. J. Antigen-presenting cell function in induction of helper T cells for cytotoxic T-lymphocyte responses: evidence for antigen processing. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1796–1799. doi: 10.1073/pnas.78.3.1796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yamashita U., Shevach E. M. The expression of Ia antigens on immunocompetent cells in the guinea pig. II. Ia antigens on macrophages. J Immunol. 1977 Nov;119(5):1584–1588. [PubMed] [Google Scholar]
  26. Yano A., Schwartz R. H., Paul W. E. Antigen presentation in the murine T-lymphocyte proliferative response. I. Requirement for genetic identity at the major histocompatibility complex. J Exp Med. 1977 Sep 1;146(3):828–843. doi: 10.1084/jem.146.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES