Abstract
In an effort to evaluate the role of the thymus in influencing the development of Lyb-5- B lymphocytes, mice expressing both the xid and nu gene defects were studied. Mice expressing either of these defects respond to both trinitrophenylated Brucellus abortus and lipopolysaccharide; whereas mice with the combined defect show markedly suppressed responses. The other abnormalities included: (a) greater than 80 percent diminution of serum Ig levels; (b) significant increase in the number of sIgM+ sIgD- B lymphocytes; (c) reduced expression of IgD on sIgD+ cells; and (d) a strikingly abnormal histology of their lymphoid tissue. Because nu/nu mice that do not express the xid defect appear relatively normal, it would suggest that the development of Lyb- 5- B lymphocytes require a thymic influence for normal maturation, whereas, Lyb-5+ B lymphocytes are relatively independent of such influences.
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- Ahmed A., Scher I., Sharrow S. O., Smith A. H., Paul W. E., Sachs D. H., Sell K. W. B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens. J Exp Med. 1977 Jan 1;145(1):101–110. doi: 10.1084/jem.145.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Amsbaugh D. F., Hansen C. T., Prescott B., Stashak P. W., Asofsky R., Baker P. J. Genetic control of the antibody response to type 3 pneumococcal polysaccharide in mice. II. Relationship between IgM immunoglobulin levels and the ability to give an IgM antibody response. J Exp Med. 1974 Jun 1;139(6):1499–1512. doi: 10.1084/jem.139.6.1499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Amsbaugh D. F., Hansen C. T., Prescott B., Stashak P. W., Barthold D. R., Baker P. J. Genetic control of the antibody response to type 3 pneumococcal polysaccharide in mice. I. Evidence that an X-linked gene plays a decisive role in determining responsiveness. J Exp Med. 1972 Oct 1;136(4):931–949. doi: 10.1084/jem.136.4.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Azar H. A., Hansen C. T., Costa J. N:NIH(S)-nu/nu mice with combined immunodeficiency: a new model for human tumor heterotransplantation. J Natl Cancer Inst. 1980 Aug;65(2):421–430. [PubMed] [Google Scholar]
- Boswell H. S., Ahmed A., Scher I., Singer A. Role of accessory cells in B cell activation. II. The interaction of B cells with accessory cells results in the exclusive activation of an Lyb5+ B cell subpopulation. J Immunol. 1980 Sep;125(3):1340–1348. [PubMed] [Google Scholar]
- Finkelman F. D., Smith A. H., Scher I., Paul W. E. Abnormal ratio of membrane immunoglobulin classes in mice with an X-linked B-lymphocyte defect. J Exp Med. 1975 Nov 1;142(5):1316–1321. doi: 10.1084/jem.142.5.1316. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gillis S., Union N. A., Baker P. E., Smith K. A. The in vitro generation and sustained culture of nude mouse cytolytic T-lymphocytes. J Exp Med. 1979 Jun 1;149(6):1460–1476. doi: 10.1084/jem.149.6.1460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glimcher L., Shen F. W., Cantor H. Identification of a cell-surface antigen selectively expressed on the natural killer cell. J Exp Med. 1977 Jan 1;145(1):1–9. doi: 10.1084/jem.145.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ihle J. N., Pepersack L., Rebar L. Regulation of T cell differentiation: in vitro induction of 20 alpha-hydroxysteroid dehydrogenase in splenic lymphocytes from athymic mice by a unique lymphokine. J Immunol. 1981 Jun;126(6):2184–2189. [PubMed] [Google Scholar]
- Kessler S. W. Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent. J Immunol. 1976 Nov;117(5 Pt 1):1482–1490. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
- Loken M. R., Herzenber L. A. Analysis of cell populations with a fluorescence-activated cell sorter. Ann N Y Acad Sci. 1975 Jun 30;254:163–171. doi: 10.1111/j.1749-6632.1975.tb29166.x. [DOI] [PubMed] [Google Scholar]
- MacDonald H. R., Lees R. K., Sordat B., Zaech P., Maryanski J. L., Bron C. Age-associated increase in expression of the T cell surface markers Thy-1, Lyt-1, and Lyt-2 in congenitally athymic (nu/nu) mice: analysis by flow microfluorometry. J Immunol. 1981 Mar;126(3):865–870. [PubMed] [Google Scholar]
- Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mond J. J., Kessler S., Finkelman F. D., Paul W. E., Scher I. Heterogeneity of Ia expression on normal B cells, neonatal B cells, and on cells from B cell-defective CBA/N mice. J Immunol. 1980 Apr;124(4):1675–1682. [PubMed] [Google Scholar]
- Mond J. J., Mongini P. K., Sieckmann D., Paul W. E. Role of T lymphocytes in the response to TNP-AECM-Ficoll. J Immunol. 1980 Sep;125(3):1066–1070. [PubMed] [Google Scholar]
- Mond J. J., Scher I., Mosier D. E., Baese M., Paul W. E. T-independent responses in B cell-defective CBA/N mice to Brucella abortus and to trinitrophenyl (TNP) conjugates of Brucella abortus. Eur J Immunol. 1978 Jul;8(7):459–463. doi: 10.1002/eji.1830080703. [DOI] [PubMed] [Google Scholar]
- Mond J. J., Stein K. E., Subbarao B., Paul W. E. Analysis of B cell activation requirements with TNP-conjugated polyacrylamide beads. J Immunol. 1979 Jul;123(1):239–245. [PubMed] [Google Scholar]
- Mosier D. E., Scher I., Paul W. E. In vitro responses of CBA/N mice: spleen cells of mice with an X-linked defect that precludes immune responses to several thymus-independent antigens can respond to TNP-lipopolysaccharide. J Immunol. 1976 Oct;117(4):1363–1369. [PubMed] [Google Scholar]
- Mosier D. E., Zitron I. M., Mond J. J., Ahmed A., Scher I., Paul W. E. Surface immunoglobulin D as a functional receptor for a subclass of B lymphocytes. Immunol Rev. 1977;37:89–104. doi: 10.1111/j.1600-065x.1977.tb00246.x. [DOI] [PubMed] [Google Scholar]
- Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
- Perlmutter R. M., Nahm M., Stein K. E., Slack J., Zitron I., Paul W. E., Davie J. M. Immunoglobulin subclass-specific immunodeficiency in mice with an X-linked B-lymphocyte defect. J Exp Med. 1979 Apr 1;149(4):993–998. doi: 10.1084/jem.149.4.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
- Scher I., Berning A. K., Kessler S., Finkelman F. D. Development of B lymphocytes in the mouse; studies of the frequency and distribution of surface IgM and IgD in normal and immune-defective CBA/N F1 mice. J Immunol. 1980 Oct;125(4):1686–1693. [PubMed] [Google Scholar]
- Scher I., Steinberg A. D., Berning A. K., Paul W. E. X-linked B-lymphocyte immune defect in CBA/N mice. II. Studies of the mechanisms underlying the immune defect. J Exp Med. 1975 Sep 1;142(3):637–650. doi: 10.1084/jem.142.3.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharon R., McMaster P. R., Kask A. M., Owens J. D., Paul W. E. DNP-Lys-ficoll: a T-independent antigen which elicits both IgM and IgG anti-DNP antibody-secreting cells. J Immunol. 1975 May;114(5):1585–1589. [PubMed] [Google Scholar]
- Subbarao B., Ahmed A., Paul W. E., Scher I., Lieberman R., Mosier D. E. Lyb-7, a new B cell alloantigen controlled by genes linked to the IgCH locus. J Immunol. 1979 Jun;122(6):2279–2285. [PubMed] [Google Scholar]
- Subbarao B., Mosier D. E., Ahmed A., Mond J. J., Scher I., Paul W. E. Role of a nonimmunoglobulin cell surface determinant in the activation of B lymphocytes by thymus-independent antigens. J Exp Med. 1979 Feb 1;149(2):495–506. doi: 10.1084/jem.149.2.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vitetta E. S., Melcher U., McWilliams M., Lamm M. E., Phillips-Quagliata J. M., Uhr J. W. Cell surface immunoglobulin. XI. The appearance of an IgD-like molecule on murine lymphoid cells during ontogeny. J Exp Med. 1975 Jan 1;141(1):206–215. doi: 10.1084/jem.141.1.206. [DOI] [PMC free article] [PubMed] [Google Scholar]