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. 1982 Apr 1;155(4):1133–1147. doi: 10.1084/jem.155.4.1133

GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody

PMCID: PMC2186649  PMID: 7061953

Abstract

Mouse monoclonal antibody AbR24 has a high degree of specificity for human melanoma cells when tested on viable cultured cells using the protein A mixed hemagglutinin serological assay. The antigen detected by this antibody has been isolated from melanoma cells and shown to be GD3 ganglioside by compositional and partial structural analysis and by comparison with authentic GD3 in thin layer chromatography (TLC). AbR24 reacts with authentic GD3, but not with any other ganglioside tested. Using TLC and reactivity with AbR24, a wide range of cells and tissues was examined for the presence of GD3. A new serological assay, termed glycolipid-mediated immune adherence, was devised for assaying the reactivity of AbR24 with gangliosides. Melanomas (cultured cells or tumor tissue) were shown to have GD3 and GM3 as major gangliosides. Other cells and tissues examined also contained GD3, but usually only in low amounts. Melanomas (and MOLT-4, a T cell line) were characterized by a simplified ganglioside profile with GD3 and GM3 as major components. The apparent discrepancy between the ubiquitous presence of GD3 and the serological specificity of AbR24 for melanoma cells can be explained in terms of localization and concentration of GD3 in different cells.

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Selected References

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  1. Carey T. E., Takahashi T., Resnick L. A., Oettgen H. F., Old L. J. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3278–3282. doi: 10.1073/pnas.73.9.3278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dippold W. G., Lloyd K. O., Li L. T., Ikeda H., Oettgen H. F., Old L. J. Cell surface antigens of human malignant melanoma: definition of six antigenic systems with mouse monoclonal antibodies. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6114–6118. doi: 10.1073/pnas.77.10.6114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eisenbarth G. S., Walsh F. S., Nirenberg M. Monoclonal antibody to a plasma membrane antigen of neurons. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4913–4917. doi: 10.1073/pnas.76.10.4913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  5. Hakomori S. Glycolipids of tumor cell membrane. Adv Cancer Res. 1973;18:265–315. doi: 10.1016/s0065-230x(08)60755-1. [DOI] [PubMed] [Google Scholar]
  6. Irwin L. N., Michael D. B., Irwin C. C. Ganglioside patterns of fetal rat and mouse brain. J Neurochem. 1980 Jun;34(6):1527–1530. doi: 10.1111/j.1471-4159.1980.tb11235.x. [DOI] [PubMed] [Google Scholar]
  7. Keenan T. W., Morré D. J., Basu S. Ganglioside biosynthesis. Concentration of glycosphingolipid glycosyltransferases in Golgi apparatus from rat liver. J Biol Chem. 1974 Jan 10;249(1):310–315. [PubMed] [Google Scholar]
  8. Kundu S. K., Marcus D. M., Veh R. W. Preparation and properties of antibodies to GD3 and GM1 gangliosides. J Neurochem. 1980 Jan;34(1):184–188. doi: 10.1111/j.1471-4159.1980.tb04638.x. [DOI] [PubMed] [Google Scholar]
  9. Lloyd K. O., Travassos L. R., Takahashi T., Old L. J. Cell surface glycoproteins of human tumor cell lines: unusual characteristics of malignant melanoma. J Natl Cancer Inst. 1979 Sep;63(3):623–634. doi: 10.1093/jnci/63.3.623. [DOI] [PubMed] [Google Scholar]
  10. Magnani J. L., Smith D. F., Ginsburg V. Detection of gangliosides that bind cholera toxin: direct binding of 125I-labeled toxin to thin-layer chromatograms. Anal Biochem. 1980 Dec;109(2):399–402. doi: 10.1016/0003-2697(80)90667-3. [DOI] [PubMed] [Google Scholar]
  11. Pacuszka T., Duffard R. O., Nishimura R. N., Brady R. O., Fishman P. H. Biosynthesis of bovine thyroid gangliosides. J Biol Chem. 1978 Aug 25;253(16):5839–5846. [PubMed] [Google Scholar]
  12. Pfreundschuh M., Shiku H., Takahashi T., Ueda R., Ransohoff J., Oettgen H. F., Old L. J. Serological analysis of cell surface antigens of malignant human brain tumors. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5122–5126. doi: 10.1073/pnas.75.10.5122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Portoukalian J., Zwingelstein G., Doré J. F. Lipid composition of human malignant melanoma tumors at various levels of malignant growth. Eur J Biochem. 1979 Feb 15;94(1):19–23. doi: 10.1111/j.1432-1033.1979.tb12866.x. [DOI] [PubMed] [Google Scholar]
  14. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rapport M. M., Graf L. Immunochemical reactions of lipids. Prog Allergy. 1969;13:273–331. [PubMed] [Google Scholar]
  16. SVENNERHOLM L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957 Jun;24(3):604–611. doi: 10.1016/0006-3002(57)90254-8. [DOI] [PubMed] [Google Scholar]
  17. Saito T., Hakomori S. I. Quantitative isolation of total glycosphingolipids from animal cells. J Lipid Res. 1971 Mar;12(2):257–259. [PubMed] [Google Scholar]
  18. Silver H. K., Karim K. A., Archibald E. L., Salinas F. A. Serum sialic acid and sialyltransferase as monitors of tumor burden in malignant melanoma patients. Cancer Res. 1979 Dec;39(12):5036–5042. [PubMed] [Google Scholar]
  19. Tamir H., Brunner W., Casper D., Rapport M. M. Enhancement by gangliosides of the binding of serotonin to serotonin binding protein. J Neurochem. 1980 Jun;34(6):1719–1724. doi: 10.1111/j.1471-4159.1980.tb11266.x. [DOI] [PubMed] [Google Scholar]
  20. Ueda R., Shiku H., Pfreundschuh M., Takahashi T., Li L. T., Whitmore W. F., Oettgen H. F., Old L. J. Cell surface antigens of human renal cancer defined by autologous typing. J Exp Med. 1979 Sep 19;150(3):564–579. doi: 10.1084/jem.150.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Urban P. F., Harth S., Freysz L., Dreyfus H. Brain and retinal ganglioside composition from different species determined by TLC and HPTLC. Adv Exp Med Biol. 1980;125:149–157. doi: 10.1007/978-1-4684-7844-0_14. [DOI] [PubMed] [Google Scholar]
  22. Watanabe K., Powell M. E., Hakomori S. I. Isolation and characterization of gangliosides with a new sialosyl linkage and core structures. II. Gangliosides of human erythrocyte membranes. J Biol Chem. 1979 Sep 10;254(17):8223–8229. [PubMed] [Google Scholar]
  23. Woolley D. W., Gommi B. W. Serotonin receptors, VII. Activities of various pure gangliosides as the receptors. Proc Natl Acad Sci U S A. 1965 May;53(5):959–963. doi: 10.1073/pnas.53.5.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yu R. K., Ando S. Structures of some new complex gangliosides of fish brain. Adv Exp Med Biol. 1980;125:33–45. doi: 10.1007/978-1-4684-7844-0_5. [DOI] [PubMed] [Google Scholar]
  25. Yu R. K., Ledeen R. W. Gangliosides of human, bovine, and rabbit plasma. J Lipid Res. 1972 Sep;13(5):680–686. [PubMed] [Google Scholar]
  26. Zanetta J. P., Breckenridge W. C., Vincendon G. Analysis of monosaccharides by gas-liquid chromatography of the O-methyl glycosides as trifluoroacetate derivatives. Application to glycoproteins and glycolipids. J Chromatogr. 1972 Jul 5;69(2):291–304. doi: 10.1016/s0021-9673(00)92897-8. [DOI] [PubMed] [Google Scholar]

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