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. 1982 May 1;155(5):1439–1451. doi: 10.1084/jem.155.5.1439

HLA-Dw2: a genetic marker for human immune response to short ragweed pollen allergen Ra5. I. Response resulting primarily from natural antigenic exposure

PMCID: PMC2186667  PMID: 6951003

Abstract

Ultra-pure short ragweed pollen allergen Ra5 (5,000 mol wt) was used to investigate the relationship between human leukocyte antigen (HLA) type and IgE and IgG antibody (Ab) responses to Ra5 in two groups of Caucasian subjects, totaling 447 people. Using highly sensitive radioimmunoassay procedures to measure serum IgE and IgG Ab, qualitative responses to Ra5 in both groups were found to be strongly associated with HLA-Dw2 (P less than 0.0001). For example, 95% of 38 people with IgE Ab vs. 22% of 139 ragweed-allergic persons having no detectable IgE Ab to Ra5 were Dw2+. Quantitative log [IgE Ab] and log[IgG Ab] responses to Ra5 were highly correlated with Dw2 (P = 10(- 5) to 10(-14)) in four separate multiple regression analyses, examining the relationship between HLA type (and other variables) and Ab levels in the two study groups. Further studies showed that the primary association of Ra5 response was with Dw2 rather than DR2 and that various combinations of A3, B7, and Dw2 were less strongly associated than Dw2 alone.

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Selected References

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  1. Bias W. B., Hsu S. H., Pollard M. K., Harvey J., Lotze M. T., Arnett F. C., Stevens M. B. HLA-DR characterization of a Chippewa Indian subpopulation with high prevalence of rheumatoid arthritis. Hum Immunol. 1981 Mar;2(2):155–163. doi: 10.1016/0198-8859(81)90062-8. [DOI] [PubMed] [Google Scholar]
  2. Chapman M. D., Platts-Mills T. A., Gabriel M., Ng H. K., Allan W. G., Hill L. E., Nunn A. J. Antibody response following prolonged hyposensitization with Dermatophagoides pteronyssinus extract. Int Arch Allergy Appl Immunol. 1980;61(4):431–440. doi: 10.1159/000232471. [DOI] [PubMed] [Google Scholar]
  3. Freidhoff L. R., Meyers D. A., Bias W. B., Chase G. A., Hussain R., Marsh D. G. A genetic-epidemiologic study of human immune responsiveness to allergens in an industrial population: I. Epidemiology of reported allergy and skin-test positivity. Am J Med Genet. 1981;9(4):323–340. doi: 10.1002/ajmg.1320090409. [DOI] [PubMed] [Google Scholar]
  4. Goodfriend L., Santilli J., Jr, Schacter B., Bias W. B., Marsh D. G. HLA-B7 cross-reacting group and human IgE-mediated sensitivity to ragweed allergen Ra5. Monogr Allergy. 1977;11:80–82. [PubMed] [Google Scholar]
  5. HALDANE J. B. The estimation and significance of the logarithm of a ratio of frequencies. Ann Hum Genet. 1956 May;20(4):309–311. doi: 10.1111/j.1469-1809.1955.tb01285.x. [DOI] [PubMed] [Google Scholar]
  6. KING T. P., NORMAN P. S. Isolation studies of allergens from regweed pollen. Biochemistry. 1962 Jul;1:709–720. doi: 10.1021/bi00910a027. [DOI] [PubMed] [Google Scholar]
  7. Lapkoff C. B., Goodfriend L. Isolation of a low molecular weight ragweed pollen allergen: Ra5. Int Arch Allergy Appl Immunol. 1974;46(2):215–229. doi: 10.1159/000231125. [DOI] [PubMed] [Google Scholar]
  8. Løwenstein H., King T. P., Goodfriend L., Hussain R., Roebber M., Marsh D. G. Antigens of Ambrosia elatior (short ragweed) pollen. II. Immunochemical identification of known antigens by quantitative immunoelectrophoresis. J Immunol. 1981 Aug;127(2):637–642. [PubMed] [Google Scholar]
  9. Marsh D. G., Bias W. B., Hsu S. H., Goodfriend L. Association of the HL-A7 cross-reacting group with a specific reaginic antibody response in allergic man. Science. 1973 Feb 16;179(4074):691–693. doi: 10.1126/science.179.4074.691. [DOI] [PubMed] [Google Scholar]
  10. Marsh D. G., Bias W. B., Santilli J., Jr, Schacter B., Goodfriend L. Ragweed allergen Ra5: a new tool in understanding the genetics and immunochemistry of immune response in man. Immunochemistry. 1975 Jul;12(6-7):539–543. doi: 10.1016/0019-2791(75)90081-6. [DOI] [PubMed] [Google Scholar]
  11. Marsh D. G., Hsu S. H., Hussain R., Meyers D. A., Freidhoff L. R., Bias W. B. Genetics of human immune response to allergens. J Allergy Clin Immunol. 1980 May;65(5):322–332. doi: 10.1016/0091-6749(80)90208-0. [DOI] [PubMed] [Google Scholar]
  12. Marsh D. G., Meyers D. A., Bias W. B. The epidemiology and genetics of atopic allergy. N Engl J Med. 1981 Dec 24;305(26):1551–1559. doi: 10.1056/NEJM198112243052603. [DOI] [PubMed] [Google Scholar]
  13. Marsh D. G., Meyers D. A., Freidhoff L. R., Ehrlich-Kautzky E., Roebber M., Norman P. S., Hsu S. H., Bias W. B. HLA-Dw2: a genetic marker for human immune response to short ragweed pollen allergen Ra5. II. Response after ragweed immunotherapy. J Exp Med. 1982 May 1;155(5):1452–1463. doi: 10.1084/jem.155.5.1452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marsh D. G., Meyers D. A., Freidhoff L. R., Hussain R., Hsu S. H., Bias W. B. Association of HLA phenotypes A1, B8, DW3 and A3, B7, DW2 with allergy. Int Arch Allergy Appl Immunol. 1981;66 (Suppl 1):48–50. doi: 10.1159/000232866. [DOI] [PubMed] [Google Scholar]
  15. Mole L. E., Goodfriend L., Lapkoff C. B., Kehoe J. M., Capra J. D. The amino acid sequence of ragweed pollen allergen Ra5. Biochemistry. 1975 Mar 25;14(6):1216–1220. doi: 10.1021/bi00677a019. [DOI] [PubMed] [Google Scholar]
  16. Norman P. S., Lichtenstein L. M. Capacity of purified antigens and whole pollen extracts to release histamine from leukocytes of hay fever patients. J Allergy Clin Immunol. 1973 Aug;52(2):94–104. doi: 10.1016/0091-6749(73)90081-x. [DOI] [PubMed] [Google Scholar]
  17. Norman P. S., Lichtenstein L. M., Ishizaka K. Diagnostic tests in ragweed hay fever. A comparison of direct skin tests, IgE antibody measurements, and basophil histamine release. J Allergy Clin Immunol. 1973 Oct;52(4):210–224. doi: 10.1016/0091-6749(73)90059-6. [DOI] [PubMed] [Google Scholar]
  18. Norman P. S., Lichtenstein L. M., Marsh D. G. Studies on allergoids from naturally occurring allergens. IV. Efficacy and safety of long-term allergoid treatment of ragweed hay fever. J Allergy Clin Immunol. 1981 Dec;68(6):460–470. doi: 10.1016/0091-6749(81)90200-1. [DOI] [PubMed] [Google Scholar]
  19. Norman P. S., Lichtenstein L. M. The clinical and immunologic specificity of immunotherapy. J Allergy Clin Immunol. 1978 Jun;61(6):370–377. doi: 10.1016/0091-6749(78)90116-1. [DOI] [PubMed] [Google Scholar]
  20. Platts-Mills T. A., Chapman M. D., Marsh D. G. Human immunoglobulin E and immunoglobulin G antibody responses to the "minor" ragweed allergen Ra3: correlation with skin tests and comparison with other allergens. J Allergy Clin Immunol. 1981 Feb;67(2):129–134. doi: 10.1016/0091-6749(81)90008-7. [DOI] [PubMed] [Google Scholar]
  21. Platts-Mills T. A., Snajdr M. J., Ishizaka K., Frankland A. W. Measurement of IgE antibody by an antigen-binding assay: correlation with PK activity and IgG and IgA antibodies to allergens. J Immunol. 1978 Apr;120(4):1201–1210. [PubMed] [Google Scholar]
  22. Roebber M., Marsh D. G., Goodfriend L. An improved procedure for isolation of ragweed pollen allergen Ra5. J Immunol. 1975 Jul;115(1):303–304. [PubMed] [Google Scholar]
  23. Santilli J., Jr, Potsus R. L., Goodfriend L., Marsh D. G. Skin reactivity to purified pollen allergens in highly ragweed-sensitive individuals. J Allergy Clin Immunol. 1980 Jun;65(6):406–412. doi: 10.1016/0091-6749(80)90232-8. [DOI] [PubMed] [Google Scholar]
  24. Schellenberg R. R., Adkinson N. F., Jr Measurement of absolute amounts of antigen-specific human IgE by a radioallergosorbent test (RAST) elution technique. J Immunol. 1975 Dec;115(6):1577–1583. [PubMed] [Google Scholar]
  25. Suciu-Foca N., Godfrey M., Khan G., Woodward K., Rohowsky C., Reed E., Hardy M., Reemtsma K. New HLA-D alleles associated with DR1 and DR2. Tissue Antigens. 1981 Mar;17(3):294–302. doi: 10.1111/j.1399-0039.1981.tb00704.x. [DOI] [PubMed] [Google Scholar]
  26. van Rood J. J., van Leeuwen A., Ploem J. S. Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants. Nature. 1976 Aug 26;262(5571):795–797. doi: 10.1038/262795a0. [DOI] [PubMed] [Google Scholar]

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