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. 1982 Jun 1;155(6):1702–1718. doi: 10.1084/jem.155.6.1702

Streptococcal cell walls and synovial cell activation. Stimulation of synovial fibroblast plasminogen activator activity by monocytes treated with group A streptococcal cell wall sonicates and muramyl dipeptide

PMCID: PMC2186715  PMID: 6804590

Abstract

Group A streptococcal peptidoglycan has previously been shown to be arthritogenic in rats and has been implicated as a structure present in a class of possible etiologic agents for rheumatoid arthritis. The present study reports that conditioned medium from human monocytes, after interaction with cell wall sonicates of four group A streptococcal strains, stimulates the plasminogen activator (PA) activity of nonrheumatoid synovial fibroblasts. Low concentrations of N- acetylmuramyl-L-alanyl-D isoglutamine (muramyl dipeptide) can also generate this synovial activator (SA) activity from human monocytes. Preliminary biochemical data suggest that the SA activity is distinct from interferon-gamma, interleukin 1, and interleukin 2. These results indicate that agents that are arthritogenic in rats can modulate human synovial fibroblast functions via monocytes. The findings are proposed to have possible significance for an understanding of the cellular interactions involved in the formation and function of the rheumatoid pannus, because PA has been invoked as possibly being generally important for the processes of cell migration, tissue remodeling, and inflammation.

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Selected References

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  1. Beers W. H. Follicular plasminogen and plasminogen activator and the effect of plasmin on ovarian follicle wall. Cell. 1975 Nov;6(3):379–386. doi: 10.1016/0092-8674(75)90187-7. [DOI] [PubMed] [Google Scholar]
  2. Bennett J. C. The infectious etiology of rheumatoid arthritis. New considerations. Arthritis Rheum. 1978 Jun;21(5):531–538. doi: 10.1002/art.1780210507. [DOI] [PubMed] [Google Scholar]
  3. Braun D. G., Holm S. E. Streptococcal anti-group A precipitins in sera from patients with rheumatic arthritis and acute glomerulonephritis. Int Arch Allergy Appl Immunol. 1970;37(2):216–224. doi: 10.1159/000230234. [DOI] [PubMed] [Google Scholar]
  4. Castor C. W., Dorstewitz E. L., Rowe K., Ritchie J. C. Abnormalities of connective tissue cells cultured from patients with rheumatoid arthritis. II. Defective regulation of hyaluronate and collagen formation. J Lab Clin Med. 1971 Jan;77(1):65–75. [PubMed] [Google Scholar]
  5. Castor C. W., Lewis R. B. Connective tissue activation. X. Current studies of the process and its mediators. Scand J Rheumatol Suppl. 1975;12:41–54. [PubMed] [Google Scholar]
  6. Cochrane C. G., Revak S. D., Wuepper K. D. Activation of Hageman factor in solid and fluid phases. A critical role of kallikrein. J Exp Med. 1973 Dec 1;138(6):1564–1583. doi: 10.1084/jem.138.6.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cromartie W. J., Craddock J. G., Schwab J. H., Anderle S. K., Yang C. H. Arthritis in rats after systemic injection of streptococcal cells or cell walls. J Exp Med. 1977 Dec 1;146(6):1585–1602. doi: 10.1084/jem.146.6.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dalldorf F. G., Cromartie W. J., Anderle S. K., Clark R. L., Schwab J. H. The relation of experimental arthritis to the distribution of streptococcal cell wall fragments. Am J Pathol. 1980 Aug;100(2):383–402. [PMC free article] [PubMed] [Google Scholar]
  9. Dayer J. M., Bréard J., Chess L., Krane S. M. Participation of monocyte-macrophages and lymphocytes in the production of a factor that stimulates collagenase and prostaglandin release by rheumatoid synovial cells. J Clin Invest. 1979 Nov;64(5):1386–1392. doi: 10.1172/JCI109596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dayer J. M., Stephenson M. L., Schmidt E., Karge W., Krane S. M. Purification of a factor from human blood monocyte-macrophages which stimulates the production of collagenase and prostaglandin E2 by cells cultured from rheumatoid synovial tissues. FEBS Lett. 1981 Feb 23;124(2):253–253. doi: 10.1016/0014-5793(81)80149-4. [DOI] [PubMed] [Google Scholar]
  11. Ellouz F., Adam A., Ciorbaru R., Lederer E. Minimal structural requirements for adjuvant activity of bacterial peptidoglycan derivatives. Biochem Biophys Res Commun. 1974 Aug 19;59(4):1317–1325. doi: 10.1016/0006-291x(74)90458-6. [DOI] [PubMed] [Google Scholar]
  12. Emori K., Tanaka A. Granuloma formation by synthetic bacterial cell wall fragment: muramyl dipeptide. Infect Immun. 1978 Feb;19(2):613–620. doi: 10.1128/iai.19.2.613-620.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fassbender H. G., Simmling-Annefeld M., Stofft E. Transformation der Synovialzellen bei rheumatoider Arthritis. Verh Dtsch Ges Pathol. 1980;64:193–212. [PubMed] [Google Scholar]
  14. Gordon S., Todd J., Cohn Z. A. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974 May 1;139(5):1228–1248. doi: 10.1084/jem.139.5.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gottlieb A. B., Fu S. M., Yu D. T., Wang C. Y., Halper J. P., Kunkel H. G. The nature of the stimulatory cell in human allogeneic and autologous MLC reactions; role of isolated IgM-bearing B cells. J Immunol. 1979 Oct;123(4):1497–1503. [PubMed] [Google Scholar]
  16. Green J. A., Cooperband S. R., Kibrick S. Immune specific induction of interferon production in cultures of human blood lymphocytes. Science. 1969 Jun 20;164(3886):1415–1417. doi: 10.1126/science.164.3886.1415. [DOI] [PubMed] [Google Scholar]
  17. Hadler N. M. A pathogenetic model for erosive synovitis: lessons from animal arthritides. Arthritis Rheum. 1976 Mar-Apr;19(2):256–266. doi: 10.1002/art.1780190222. [DOI] [PubMed] [Google Scholar]
  18. Hamilton J. A., Slywka J. Stimulation of human synovial fibroblast plasminogen activator production by mononuclear cell supernatants. J Immunol. 1981 Mar;126(3):851–855. [PubMed] [Google Scholar]
  19. Harris E. D., Jr Recent insights into the pathogenesis of the proliferative lesion in rheumatoid arthritis. Arthritis Rheum. 1976 Jan-Feb;19(1):68–72. doi: 10.1002/art.1780190111. [DOI] [PubMed] [Google Scholar]
  20. Heymer B., Schleifer K. H., Read S., Zabriskie J. B., Krause R. M. Detection of antibodies to bacterial cell wall peptidoglycan in human sera. J Immunol. 1976 Jul;117(1):23–26. [PubMed] [Google Scholar]
  21. Kaplan A. P., Austen K. F. A prealbumin activator of prekallikrein. II. Derivation of activators of prekallikrein from active Hageman factor by digestion with plasmin. J Exp Med. 1971 Apr 1;133(4):696–712. doi: 10.1084/jem.133.4.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. LACK C. H., ROGERS H. J. Action of plasmin on cartilage. Nature. 1958 Oct 4;182(4640):948–949. doi: 10.1038/182948a0. [DOI] [PubMed] [Google Scholar]
  23. Lachman L. B., Moore J. O., Metzgar R. S. Preparation and characterization of lymphocyte-activating factor (LAF) from acute monocytic and myelomonocytic leukemia cells. Cell Immunol. 1978 Nov;41(1):199–206. doi: 10.1016/s0008-8749(78)80040-9. [DOI] [PubMed] [Google Scholar]
  24. Mier J. W., Gallo R. C. Purification and some characteristics of human T-cell growth factor from phytohemagglutinin-stimulated lymphocyte-conditioned media. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6134–6138. doi: 10.1073/pnas.77.10.6134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Mizel S. B., Dayer J. M., Krane S. M., Mergenhagen S. E. Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1). Proc Natl Acad Sci U S A. 1981 Apr;78(4):2474–2477. doi: 10.1073/pnas.78.4.2474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Oppenheim J. J., Togawa A., Chedid L., Mizel S. Components of mycobacteria and muramyl dipeptide with adjuvant activity induce lymphocyte activating factor. Cell Immunol. 1980 Mar 1;50(1):71–81. doi: 10.1016/0008-8749(80)90007-6. [DOI] [PubMed] [Google Scholar]
  27. Ossowski L., Quigley J. P., Reich E. Fibrinolysis associated with oncogenic transformation. Morphological correlates. J Biol Chem. 1974 Jul 10;249(13):4312–4320. [PubMed] [Google Scholar]
  28. PARRY R. M., Jr, CHANDAN R. C., SHAHANI K. M. A RAPID AND SENSITIVE ASSAY OF MURAMIDASE. Proc Soc Exp Biol Med. 1965 Jun;119:384–386. doi: 10.3181/00379727-119-30188. [DOI] [PubMed] [Google Scholar]
  29. Page R. C., Davies P., Allison A. C. Participation of mononuclear phagocytes in chronic inflammatory diseases. J Reticuloendothel Soc. 1974 May;15(5):413–438. [PubMed] [Google Scholar]
  30. Quigley J. P., Ossowski L., Reich E. Plasminogen, the serum proenzyme activated by factors from cells transformed by oncogenic viruses. J Biol Chem. 1974 Jul 10;249(13):4306–4311. [PubMed] [Google Scholar]
  31. Ratnoff O. D., Naff G. B. The conversion of C'IS to C'1 esterase by plasmin and trypsin. J Exp Med. 1967 Feb 1;125(2):337–358. doi: 10.1084/jem.125.2.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Read S. E., Fischetti V. A., Utermohlen V., Falk R. E., Zabriskie J. B. Cellular reactivity studies to streptococcal antigens. Migration inhibition studies in patients with streptococcal infections and rheumatic fever. J Clin Invest. 1974 Aug;54(2):439–450. doi: 10.1172/JCI107780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Schwab J. H., Cromartie W. J., Ohanian S. H., Craddock J. G. Association of experimental chronic arthritis with the persistence of group A streptococcal cell walls in the articular tissue. J Bacteriol. 1967 Nov;94(5):1728–1735. doi: 10.1128/jb.94.5.1728-1735.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Shah R. G., Caporale L. H., Moore M. A. Characterization of colony-stimulating activity produced by human monocytes and phytohemagglutinin-stimulated lymphocytes. Blood. 1977 Nov;50(5):811–821. [PubMed] [Google Scholar]
  35. Smith K. A. T-cell growth factor. Immunol Rev. 1980;51:337–357. doi: 10.1111/j.1600-065x.1980.tb00327.x. [DOI] [PubMed] [Google Scholar]
  36. Vassalli J. D., Hamilton J., Reich E. Macrophage plasminogen activator: modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotides. Cell. 1976 Jun;8(2):271–281. doi: 10.1016/0092-8674(76)90011-8. [DOI] [PubMed] [Google Scholar]
  37. Wahl S. M., Wahl L. M., McCarthy J. B., Chedid L., Mergenhagen S. E. Macrophage activation by mycobacterial water soluble compounds and synthetic muramyl dipeptide. J Immunol. 1979 Jun;122(6):2226–2231. [PubMed] [Google Scholar]
  38. Werb Z., Mainardi C. L., Vater C. A., Harris E. D., Jr Endogenous activiation of latent collagenase by rheumatoid synovial cells. Evidence for a role of plasminogen activator. N Engl J Med. 1977 May 5;296(18):1017–1023. doi: 10.1056/NEJM197705052961801. [DOI] [PubMed] [Google Scholar]
  39. Wheelock E. F. Interferon-like virus-inhibitor induced in human leukocytes by phytohemagglutinin. Science. 1965 Jul 16;149(3681):310–311. [PubMed] [Google Scholar]
  40. Wilson E. L., Dowdle E. Secretion of plasminogen activator by normal, reactive and neoplastic human tissues cultured in vitro. Int J Cancer. 1978 Oct 15;22(4):390–399. doi: 10.1002/ijc.2910220405. [DOI] [PubMed] [Google Scholar]
  41. Wiranowska-Stewart M., Lin L. S., Braude I. A., Stewart W. E., 2nd Production, partial purification and characterization of human and murine interferons--type II. Mol Immunol. 1980 May;17(5):625–633. doi: 10.1016/0161-5890(80)90160-1. [DOI] [PubMed] [Google Scholar]
  42. Zabriskie J. B., Freimer E. H. An immunological relationship between the group. A streptococcus and mammalian muscle. J Exp Med. 1966 Oct 1;124(4):661–678. doi: 10.1084/jem.124.4.661. [DOI] [PMC free article] [PubMed] [Google Scholar]

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